1. Cell Cycle/DNA Damage
    Cytoskeleton
    Antibody-drug Conjugate/ADC Related
  2. Microtubule/Tubulin
    ADC Cytotoxin
  3. MMAF Hydrochloride

MMAF Hydrochloride (Synonyms: Monomethylauristatin F Hydrochloride)

Cat. No.: HY-15579A Purity: 99.89%
Handling Instructions

MMAF Hydrochloride (Monomethylauristatin F Hydrochloride) is a potent tubulin polymerization inhibitor and is used as a antitumor agent. MMAF Hydrochloride (Monomethylauristatin F Hydrochloride) is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) such as Vorsetuzumab mafodotin and SGN-CD19A.

For research use only. We do not sell to patients.

MMAF Hydrochloride Chemical Structure

MMAF Hydrochloride Chemical Structure

CAS No. : 1415246-68-2

Size Price Stock Quantity
2 mg USD 180 In-stock
Estimated Time of Arrival: December 31
5 mg USD 336 In-stock
Estimated Time of Arrival: December 31
10 mg USD 528 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of MMAF Hydrochloride:

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

MMAF Hydrochloride (Monomethylauristatin F Hydrochloride) is a potent tubulin polymerization inhibitor and is used as a antitumor agent. MMAF Hydrochloride (Monomethylauristatin F Hydrochloride) is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) such as Vorsetuzumab mafodotin and SGN-CD19A[1][2][3].

IC50 & Target

Auristatin

 

In Vitro

MMAF inhibits anaplastic large cell lymphoma Karpas 299, breast carcinoma H3396, renal cell carcinoma 786-O and Caki-1 cells with IC50s of 119, 105, 257 and 200 nM in vitro cytotoxicity assay[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

768.42

Formula

C₃₉H₆₆ClN₅O₈

CAS No.

1415246-68-2

SMILES

CC(C)[[email protected]](NC)C(N[[email protected]](C(N([[email protected]@H]([[email protected]@H](C)CC)[[email protected]](OC)CC(N1CCC[[email protected]@]1([H])[[email protected]](OC)[[email protected]@H](C)C(N[[email protected]](C(O)=O)CC2=CC=CC=C2)=O)=O)C)=O)C(C)C)=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 27 mg/mL (35.14 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3014 mL 6.5069 mL 13.0137 mL
5 mM 0.2603 mL 1.3014 mL 2.6027 mL
10 mM 0.1301 mL 0.6507 mL 1.3014 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice: When subcutaneous Karpas 299 tumor size reaches 300 mm3, three animals per group receives one injection of 10 mg antibody component/kg body weight of either cAC10-L1-MMAF4 or cBR96-L1-MMAF4 intravenously. Tumors are then removed and placed in optimal cutting temperature compound, and 5 μm-thin frozen tissue sections are stained using immunohistochemistry evaluation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.89%

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Keywords:

MMAFMonomethylauristatin FMicrotubule/TubulinADC CytotoxinInhibitorinhibitorinhibit

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MMAF Hydrochloride
Cat. No.:
HY-15579A
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