MMAF-d8 hydrochloride
Based on 1 Customer Validation
MMAF-d8 (hydrochloride)e is a deuterated form of MMAF hydrochloride, which is a microtubule disrupting agent.
For research use only. We do not sell to patients.
- Purity: 99.52%
- Formula: C39H58D8ClN5O8
- Molecular Weight:776.47
-
Storage:
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocol
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
Auristatin |
MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
-
Unlabeled Cas 1415246-68-2
-
Appearance Solid
-
Molecular Weight 776.47
-
Formula C39H58D8ClN5O8
-
Color White to off-white
-
SMILES
O=C(O)[C@H](CC1=CC=CC=C1)NC([C@H](C)[C@@H](OC)[C@H]2N(C(C[C@@H](OC)[C@@H](N(C)C([C@@]([2H])(NC([C@@H](NC)C(C)C)=O)C([2H])(C([2H])([2H])[2H])C([2H])([2H])[2H])=O)[C@@H](C)CC)=O)CCC2)=O.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
DMSO : 100 mg/mL (128.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice: When subcutaneous Karpas 299 tumor size reaches 300 mm3, three animals per group receives one injection of 10 mg antibody component/kg body weight of either cAC10-L1-MMAF4 or cBR96-L1-MMAF4 intravenously. Tumors are then removed and placed in optimal cutting temperature compound, and 5 μm-thin frozen tissue sections are stained using immunohistochemistry evaluation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
-
Data Sheet (280 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2879 mL | 6.4394 mL | 12.8788 mL | 32.1970 mL |
| 5 mM | 0.2576 mL | 1.2879 mL | 2.5758 mL | 6.4394 mL | |
| 10 mM | 0.1288 mL | 0.6439 mL | 1.2879 mL | 3.2197 mL | |
| 15 mM | 0.0859 mL | 0.4293 mL | 0.8586 mL | 2.1465 mL | |
| 20 mM | 0.0644 mL | 0.3220 mL | 0.6439 mL | 1.6098 mL | |
| 25 mM | 0.0515 mL | 0.2576 mL | 0.5152 mL | 1.2879 mL | |
| 30 mM | 0.0429 mL | 0.2146 mL | 0.4293 mL | 1.0732 mL | |
| 40 mM | 0.0322 mL | 0.1610 mL | 0.3220 mL | 0.8049 mL | |
| 50 mM | 0.0258 mL | 0.1288 mL | 0.2576 mL | 0.6439 mL | |
| 60 mM | 0.0215 mL | 0.1073 mL | 0.2146 mL | 0.5366 mL | |
| 80 mM | 0.0161 mL | 0.0805 mL | 0.1610 mL | 0.4025 mL | |
| 100 mM | 0.0129 mL | 0.0644 mL | 0.1288 mL | 0.3220 mL |
MMAF-d8 hydrochloride Related Classifications
HY-15579AS Related Classifications
Powered by Bioz