1. Antibody-drug Conjugate/ADC Related
  2. ADC Cytotoxin

MMAF-OMe (Synonyms: Monomethyl auristatin F methyl ester)

Cat. No.: HY-79256
Handling Instructions

MMAF-Ome belongs to ADC, and inhibits several tumor cell lines with IC50s of 0.056 nM, 0.166 nM, 0.183 nM, and 0.449 nM for MDAMB435/5T4, MDAMB361DYT2, MDAMB468, and Raji (5T4-) cell lines, respectively.

For research use only. We do not sell to patients.
MMAF-OMe Chemical Structure

MMAF-OMe Chemical Structure

CAS No. : 863971-12-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 689 In-stock
2 mg USD 264 In-stock
5 mg USD 420 In-stock
10 mg USD 660 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Other Forms of MMAF-OMe:

  • Biological Activity

  • Protocol

  • Technical Information

  • References

Description

MMAF-Ome belongs to ADC, and inhibits several tumor cell lines with IC50s of 0.056 nM, 0.166 nM, 0.183 nM, and 0.449 nM for MDAMB435/5T4, MDAMB361DYT2, MDAMB468, and Raji (5T4-) cell lines, respectively.

In Vitro

2.5F-Fc and 2.5F-Fc-MMAF have similar IC50 values (6.9±1.1 vs. 8.3±1.3 nM, respectively), indicating that MMAF conjugation has negligible impact on integrin-binding affinity[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.3405 mL 6.7025 mL 13.4050 mL
5 mM 0.2681 mL 1.3405 mL 2.6810 mL
10 mM 0.1341 mL 0.6703 mL 1.3405 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Cells are seeded in a 96-well plate at a density of 2,000 cells per well and grown overnight at 37°C, 5% CO2 in the media described for each cell line above. Cells are subsequently treated with 100 μL of fresh media, containing varying concentrations of knottin-Fc fusion proteins or linker-modified MMAF, and incubated for 5 days at 37°C, 5% CO2. Cell proliferation is measured using the Cell Counting Kit-8 (CCK-8), by adding the water-soluble tetrazolium salt, WST-8, to each well in an amount equal to 10% of the culture volume. After incubation for 1 hour at 37°C, absorbance at 450 nm is measured with a Synergy H4 microtiter plate reader. Cell proliferation is expressed as a percentage of absorbance relative to the control of untreated cells. Percent maximum proliferation is then reported as (sample − background)/(control − background) × 100. Error bars represent the SD of experiments performed in triplicate. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

745.99

Formula

C₄₀H₆₇N₅O₈

CAS No.

863971-12-4

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
MMAF-OMe
Cat. No.:
HY-79256
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