Cat. No.: HY-N0290

Mangiferin

16 Cited Publications

Customer Review

Based on 16 publication(s) in Google Scholar

Mangiferin is a Nrf2 activator. Mangiferin suppresses nuclear translocation of the NF-κB subunits p65 and p50. Mangiferin exhibits antioxidant, antidiabetic, antihyperuricemic, antiviral, anticancer and antiinflammatory activities.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 4773-96-0
Formula: C₁₉H₁₈O₁₁
Molecular Weight:422.34

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Mangiferin Chemical Structure

CAS No.: 4773-96-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (16)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

p65

p50

Nrf2

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	38.5 μM Compound: 14	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay	[PMID: 24042007]
Vero	CC₅₀	>500 μg/mL Compound: Mangiferin	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31956052]
Vero	IC₅₀	2.9 μg/mL Compound: Mangiferin	Antiviral activity against ACV-resistant Herpes simplex virus 1 AR-29 infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay Antiviral activity against ACV-resistant Herpes simplex virus 1 AR-29 infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay	[PMID: 31956052]
Vero	IC₅₀	3.5 μg/mL Compound: Mangiferin	Antiviral activity against ACV-sensitive Herpes simplex virus 1 KOS infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay Antiviral activity against ACV-sensitive Herpes simplex virus 1 KOS infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay	[PMID: 31956052]

In Vitro

Mangiferin is glucosylxanthone extracted from plants of the Anacardiaceae and Gentianaceae families. Mangiferin (50 μM) significantly increases Nrf2 protein accumulation in HL-60 cells, particularly in the nucleus. Mangiferin also enhances the binding of Nrf2 to an antioxidant response element (ARE), significantly up-regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) expression and reduces intracellular ROS in HL60 cells. Mangiferin alone dose-dependently inhibits the proliferation of HL-60 cells. In mononuclear cells (MNCs), Mangiferin significantly relieves oxidative stress, but attenuates etoposide-induced cytotoxicity^[1]. Mangiferin is a natural phytopolyphenol that exhibits various pharmacological properties. Mangiferin is present in several plant species such as Mangifera indica, Iris unguicularis, and Anemarrhena asphodeloides. Mangiferin downregulates TNF-α-induced MMP-9 mRNA and protein expression by suppressing NF-κB activity, consequently suppressing the invasion of LNCaP cells.To assess whether Mangiferin influences the viability of LNCaP cells, MTT assay is performed 24 h after treatment with different concentrations of Mangiferin in the presence or absence of TNF-α in serum and serum-free conditions. There are no cytotoxic evident in LNCaP cells treated with up to 400 μM of Mangiferin alone under both serum and serum-free conditions. Additionally, in the presence of TNF-α (20 ng/mL), Mangiferin does not affect cell viability. Mangiferin (400 μM) treatment significantly decreases the NF-κB luciferase activity in TNF-α-stimulated LNCaP cells. Pretreatment with Mangiferin (400 μM) for 1 h significantly decreases TNF-α-induced NF-κB activity. The RT-PCR shows that Mangiferin (400 μM) significantly suppresses the TNF-α-induced MMP-9 expression in LNCaP cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 4773-96-0
Appearance Solid
Molecular Weight 422.34
Formula C₁₉H₁₈O₁₁
Color Off-white to yellow
SMILES

O[C@@H]([C@H](O)[C@@H](CO)O1)[C@@H](O)[C@@H]1C2=C(O)C3=C(C=C2O)OC4=CC(O)=C(O)C=C4C3=O
Structure Classification
- Phenols
- Polyphenols
- Phenols
- Xanthones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (16)

All Histological Imaging/StainingWBIFCell Migration/Invasion Assay

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Journal Impact Factor

ACS Nano

Starfish-Inspired Synergistic Reinforced Hydrogel Wound Dressing: Dual Responsiveness and Enhanced Bioactive Compound Delivery for Advanced Skin Regeneration and Management. [Abstract]

2025 Mar 18;19(10):10180-10198. PMID: 40048360
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Acta Pharmacol Sin

Mangiferin alleviates diabetic pulmonary fibrosis in mice via inhibiting endothelial-mesenchymal transition through AMPK/FoxO3/SIRT3 axis. [Abstract]

2024 May;45(5):1002-1018. PMID: 38225395

Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018. [Abstract]

Mangiferin (20/60 mg/kg). Representative images of H&E staining and Masson staining of mouse lung tissue.

Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018. [Abstract]

Mangiferin (20/60 mg/kg). Representative Western blot images of collagen 3, CD31, vimentin, and α-SMA proteins in mouse lung tissue.

Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018. [Abstract]

Mangiferin (20/60 mg/kg). Representative immunofluorescence staining image (200×) shows endothelial-mesenchymal transition (EndMT) around the blood vessels in mouse lungs.

Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018. [Abstract]

Representative images of scratch assay and cell migration rate of high glucose-induced HUVECs cells after treatment with different concentrations of Mangiferin (10/50 μM) for 0, 12 and 24 hours.

Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018. [Abstract]

Representative Western blot images of CD31, collagen 3, vimentin, phosphorylated AMPK, total AMPK, phosphorylated FoxO3, total FoxO3, SIRT3, and panacetyllysine proteins in high glucose-induced HUVECs after treatment with high doses of Mangiferin and compound C.
Phytother Res

Mangiferin prevents hepatocyte epithelial-mesenchymal transition in liver fibrosis via targeting HSP27-mediated JAK2/STAT3 and TGF-β1/Smad pathway. [Abstract]

2022 Nov;36(11):4167-4182. PMID: 35778992
Ind Crops Prod

2025 Dec 11;239:122458.
Chin Med

Combination of mangiferin and T0901317 targeting autophagy promotes cholesterol efflux from macrophage foam cell in atherosclerosis. [Abstract]

2024 Jan 5;19(1):5. PMID: 38183139
J Ethnopharmacol

Exploring the pathogenesis of type 2 diabetes mellitus intestinal damp-heat syndrome and the therapeutic effect of Gegen Qinlian Decoction from the perspective of exosomal miRNA. [Abstract]

2022 Mar 1:285:114786. PMID: 34763043
Brain Res Bull

Mangiferin targets PFKFB3 to inhibit glioblastoma progression by suppressing glycolysis and PI3K/AKT/mTOR signaling. [Abstract]

2025 Aug 25:230:111520. PMID: 40865736
J Biochem Mol Toxicol

Mangiferin activates the nuclear factor erythroid 2-related factor pathway to protect SOD1-G93A induced NSC-34 motor neurons from oxidative stress and apoptosis. [Abstract]

2024 Oct;38(10):e23849. PMID: 39264833
Arch Physiol Biochem

Mangiferin prevents glucolipotoxicity-induced pancreatic beta-cell injury through modulation of autophagy via AMPK-mTOR signaling pathway. [Abstract]

2024 Sep 3:1-10. PMID: 39225043
PLoS One

Molecular mechanism of ectopic lipid accumulation induced by methylglyoxal via activation of the NRF2/PI3K/AKT pathway implicates renal lipotoxicity caused by diabetes mellitus. [Abstract]

2024 Oct 16;19(10):e0306575. PMID: 39413106
Biochem Biophys Res Commun

Effects of intracellular iron overload on cell death and identification of potent cell death inhibitors. [Abstract]

2018 Sep 3;503(1):297-303. PMID: 29890135
SSRN

2024 Dec 31.
University of Paris

2022 Sep 19.
Oxid Med Cell Longev

Mangiferin Alleviates Mitochondrial ROS in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration via Suppression of NF- κ B Signaling Pathway. [Abstract]

2021 Jun 11:2021:6632786. PMID: 34234886

Solvent & Solubility

In Vitro:

DMSO : 31.82 mg/mL (75.34 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3678 mL

11.8388 mL

23.6776 mL

5 mM

0.4736 mL

2.3678 mL

4.7355 mL

10 mM

0.2368 mL

1.1839 mL

2.3678 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

LNCaP cells are cultured at 37°C in 5% CO₂ in RPMI supplemented with 10% FBS and antibiotics. Cell viability is determined by an MTT assay. Briefly, LNCaP cells (1×10⁵ cells/mL) are plated onto 24 well plates and incubated overnight in serum and serum-free RPMI media. The cells are treated with the indicated concentrations of Mangiferin (100, 200, and 400 μM) for 1 h and then stimulated with TNF-α (20 ng/mL) for 24 h. Then, the cells are incubated with a solution of 0.5 mg/mL MTT and incubation for 45 min at 37°C and 5% CO₂. Supernatant is removed and the formation of formazan is observed by monitoring the signal at 540 nm using a microplate reader^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang BP, et al. Mangiferin activates Nrf2-antioxidant response element signaling without reducing the sensitivity to etoposide of human myeloid leukemia cells in vitro. Acta Pharmacol Sin. 2014 Feb;35(2):257-66. [Content Brief]

[2]. Dilshara MG, et al. Mangiferin inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 expression and cellular invasion by suppressing nuclear factor-κB activity. BMB Rep. 2015 Oct;48(10):559-64. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3678 mL	11.8388 mL	23.6776 mL	59.1940 mL
	5 mM	0.4736 mL	2.3678 mL	4.7355 mL	11.8388 mL
	10 mM	0.2368 mL	1.1839 mL	2.3678 mL	5.9194 mL
	15 mM	0.1579 mL	0.7893 mL	1.5785 mL	3.9463 mL
	20 mM	0.1184 mL	0.5919 mL	1.1839 mL	2.9597 mL
	25 mM	0.0947 mL	0.4736 mL	0.9471 mL	2.3678 mL
	30 mM	0.0789 mL	0.3946 mL	0.7893 mL	1.9731 mL
	40 mM	0.0592 mL	0.2960 mL	0.5919 mL	1.4799 mL
	50 mM	0.0474 mL	0.2368 mL	0.4736 mL	1.1839 mL
	60 mM	0.0395 mL	0.1973 mL	0.3946 mL	0.9866 mL

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Mangiferin

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Publications Citing Use of MedChemExpress (MCE) Mangiferin

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (16)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Mangiferin Related Classifications

Cat. No.: HY-N0290