2. NF-κB
    Apoptosis
  3. NF-κB
    Keap1-Nrf2
    Apoptosis
  4. Mangiferin

Mangiferin 

Cat. No.: HY-N0290 Purity: 99.98%
COA Handling Instructions

Mangiferin is a Nrf2 activator. Mangiferin suppresses nuclear translocation of the NF-κB subunits p65 and p50. Mangiferin exhibits antioxidant, antidiabetic, antihyperuricemic, antiviral, anticancer and antiinflammatory activities.

For research use only. We do not sell to patients.

Mangiferin Chemical Structure

Mangiferin Chemical Structure

CAS No. : 4773-96-0

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 83 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 83 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 75 In-stock
25 mg USD 110 In-stock
50 mg USD 140 In-stock
100 mg USD 180 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Mangiferin is a Nrf2 activator. Mangiferin suppresses nuclear translocation of the NF-κB subunits p65 and p50. Mangiferin exhibits antioxidant, antidiabetic, antihyperuricemic, antiviral, anticancer and antiinflammatory activities[1][2][3].

IC50 & Target[1][2]

p65

 

p50

 

Nrf2

 

In Vitro

Mangiferin is glucosylxanthone extracted from plants of the Anacardiaceae and Gentianaceae families. Mangiferin (50 μM) significantly increases Nrf2 protein accumulation in HL-60 cells, particularly in the nucleus. Mangiferin also enhances the binding of Nrf2 to an antioxidant response element (ARE), significantly up-regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) expression and reduces intracellular ROS in HL60 cells. Mangiferin alone dose-dependently inhibits the proliferation of HL-60 cells. In mononuclear cells (MNCs), Mangiferin significantly relieves oxidative stress, but attenuates etoposide-induced cytotoxicity[1]. Mangiferin is a natural phytopolyphenol that exhibits various pharmacological properties. Mangiferin is present in several plant species such as Mangifera indica, Iris unguicularis, and Anemarrhena asphodeloides. Mangiferin downregulates TNF-α-induced MMP-9 mRNA and protein expression by suppressing NF-κB activity, consequently suppressing the invasion of LNCaP cells.To assess whether Mangiferin influences the viability of LNCaP cells, MTT assay is performed 24 h after treatment with different concentrations of Mangiferin in the presence or absence of TNF-α in serum and serum-free conditions. There are no cytotoxic evident in LNCaP cells treated with up to 400 μM of Mangiferin alone under both serum and serum-free conditions. Additionally, in the presence of TNF-α (20 ng/mL), Mangiferin does not affect cell viability. Mangiferin (400 μM) treatment significantly decreases the NF-κB luciferase activity in TNF-α-stimulated LNCaP cells. Pretreatment with Mangiferin (400 μM) for 1 h significantly decreases TNF-α-induced NF-κB activity. The RT-PCR shows that Mangiferin (400 μM) significantly suppresses the TNF-α-induced MMP-9 expression in LNCaP cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

422.34

Appearance

Solid

Formula

C19H18O11
CAS No.
SMILES

O[[email protected]@H]([[email protected]](O)[[email protected]@H](CO)O1)[[email protected]@H](O)[[email protected]@H]1C2=C(O)C3=C(C=C2O)OC4=CC(O)=C(O)C=C4C3=O
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 31.25 mg/mL (73.99 mM; ultrasonic and warming and heat to 80°C)

H2O : < 0.1 mg/mL (ultrasonic) (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3678 mL 11.8388 mL 23.6776 mL
5 mM 0.4736 mL 2.3678 mL 4.7355 mL
10 mM 0.2368 mL 1.1839 mL 2.3678 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.98%

COA (251 KB) HNMR (311 KB) RP-HPLC (244 KB) MS (69 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[2]

LNCaP cells are cultured at 37°C in 5% CO2 in RPMI supplemented with 10% FBS and antibiotics. Cell viability is determined by an MTT assay. Briefly, LNCaP cells (1×105 cells/mL) are plated onto 24 well plates and incubated overnight in serum and serum-free RPMI media. The cells are treated with the indicated concentrations of Mangiferin (100, 200, and 400 μM) for 1 h and then stimulated with TNF-α (20 ng/mL) for 24 h. Then, the cells are incubated with a solution of 0.5 mg/mL MTT and incubation for 45 min at 37°C and 5% CO2. Supernatant is removed and the formation of formazan is observed by monitoring the signal at 540 nm using a microplate reader[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Mangiferin4773-96-0NF-κBKeap1-Nrf2ApoptosisNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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