2. NF-κB Apoptosis
  3. NF-κB Keap1-Nrf2 Apoptosis
  4. Mangiferin

マンギフェリン  (Synonyms: Mangiferin)

製品番号: HY-N0290 純度: 99.92%
COA 取扱説明書 Technical Support

Mangiferin is a Nrf2 activator. Mangiferin suppresses nuclear translocation of the NF-κB subunits p65 and p50. Mangiferin exhibits antioxidant, antidiabetic, antihyperuricemic, antiviral, anticancer and antiinflammatory activities.

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CAS 番号 : 4773-96-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
5 mg $25 在庫あり
10 mg $33 在庫あり
25 mg $48 在庫あり
50 mg $58 在庫あり
100 mg $70 在庫あり
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カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Other Forms of Mangiferin:

マンギフェリン 関連抗体

Top Publications Citing Use of Products

    Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018.  [Abstract]

    Mangiferin (20/60 mg/kg). Representative images of H&E staining and Masson staining of mouse lung tissue.

    Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018.  [Abstract]

    Mangiferin (20/60 mg/kg). Representative Western blot images of collagen 3, CD31, vimentin, and α-SMA proteins in mouse lung tissue.

    Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018.  [Abstract]

    Mangiferin (20/60 mg/kg). Representative immunofluorescence staining image (200×) shows endothelial-mesenchymal transition (EndMT) around the blood vessels in mouse lungs.

    Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018.  [Abstract]

    Representative images of scratch assay and cell migration rate of high glucose-induced HUVECs cells after treatment with different concentrations of Mangiferin (10/50 μM) for 0, 12 and 24 hours.

    Mangiferin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):1002-1018.  [Abstract]

    Representative Western blot images of CD31, collagen 3, vimentin, phosphorylated AMPK, total AMPK, phosphorylated FoxO3, total FoxO3, SIRT3, and panacetyllysine proteins in high glucose-induced HUVECs after treatment with high doses of Mangiferin and compound C.

    NF-κB アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Mangiferin is a Nrf2 activator. Mangiferin suppresses nuclear translocation of the NF-κB subunits p65 and p50. Mangiferin exhibits antioxidant, antidiabetic, antihyperuricemic, antiviral, anticancer and antiinflammatory activities[1][2][3].

    IC50 & Target[1][2]

    p65

     

    p50

     

    Nrf2

     

    Cellular Effect
    Cell Line Type Value Description References
    BV-2 IC50
    38.5 μM
    Compound: 14
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    		[PMID: 24042007]
    Vero CC50
    > 500 μg/mL
    Compound: Mangiferin
    Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 31956052]
    Vero IC50
    2.9 μg/mL
    Compound: Mangiferin
    Antiviral activity against ACV-resistant Herpes simplex virus 1 AR-29 infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay
    Antiviral activity against ACV-resistant Herpes simplex virus 1 AR-29 infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay
    		[PMID: 31956052]
    Vero IC50
    3.5 μg/mL
    Compound: Mangiferin
    Antiviral activity against ACV-sensitive Herpes simplex virus 1 KOS infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay
    Antiviral activity against ACV-sensitive Herpes simplex virus 1 KOS infected in African green monkey Vero cells assessed as inhibition of viral infection measured after 72 hrs by crystal violet staining based plaque reduction assay
    		[PMID: 31956052]
    体外実験

    Mangiferin is glucosylxanthone extracted from plants of the Anacardiaceae and Gentianaceae families. Mangiferin (50 μM) significantly increases Nrf2 protein accumulation in HL-60 cells, particularly in the nucleus. Mangiferin also enhances the binding of Nrf2 to an antioxidant response element (ARE), significantly up-regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) expression and reduces intracellular ROS in HL60 cells. Mangiferin alone dose-dependently inhibits the proliferation of HL-60 cells. In mononuclear cells (MNCs), Mangiferin significantly relieves oxidative stress, but attenuates etoposide-induced cytotoxicity[1]. Mangiferin is a natural phytopolyphenol that exhibits various pharmacological properties. Mangiferin is present in several plant species such as Mangifera indica, Iris unguicularis, and Anemarrhena asphodeloides. Mangiferin downregulates TNF-α-induced MMP-9 mRNA and protein expression by suppressing NF-κB activity, consequently suppressing the invasion of LNCaP cells.To assess whether Mangiferin influences the viability of LNCaP cells, MTT assay is performed 24 h after treatment with different concentrations of Mangiferin in the presence or absence of TNF-α in serum and serum-free conditions. There are no cytotoxic evident in LNCaP cells treated with up to 400 μM of Mangiferin alone under both serum and serum-free conditions. Additionally, in the presence of TNF-α (20 ng/mL), Mangiferin does not affect cell viability. Mangiferin (400 μM) treatment significantly decreases the NF-κB luciferase activity in TNF-α-stimulated LNCaP cells. Pretreatment with Mangiferin (400 μM) for 1 h significantly decreases TNF-α-induced NF-κB activity. The RT-PCR shows that Mangiferin (400 μM) significantly suppresses the TNF-α-induced MMP-9 expression in LNCaP cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    マンギフェリン 関連抗体
    分子量

    422.34

    分子式

    C19H18O11
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O[C@@H]([C@H](O)[C@@H](CO)O1)[C@@H](O)[C@@H]1C2=C(O)C3=C(C=C2O)OC4=CC(O)=C(O)C=C4C3=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 31.82 mg/mL (75.34 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3678 mL 11.8388 mL 23.6776 mL
    5 mM 0.4736 mL 2.3678 mL 4.7355 mL
    10 mM 0.2368 mL 1.1839 mL 2.3678 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

    V1

    =
    濃度 （終了）

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    g

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.92%

    COA (251 KB) HNMR (190 KB) CNMR (144 KB) RP-HPLC (259 KB) MS (130 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    LNCaP cells are cultured at 37°C in 5% CO2 in RPMI supplemented with 10% FBS and antibiotics. Cell viability is determined by an MTT assay. Briefly, LNCaP cells (1×105 cells/mL) are plated onto 24 well plates and incubated overnight in serum and serum-free RPMI media. The cells are treated with the indicated concentrations of Mangiferin (100, 200, and 400 μM) for 1 h and then stimulated with TNF-α (20 ng/mL) for 24 h. Then, the cells are incubated with a solution of 0.5 mg/mL MTT and incubation for 45 min at 37°C and 5% CO2. Supernatant is removed and the formation of formazan is observed by monitoring the signal at 540 nm using a microplate reader[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3678 mL 11.8388 mL 23.6776 mL 59.1940 mL
    5 mM 0.4736 mL 2.3678 mL 4.7355 mL 11.8388 mL
    10 mM 0.2368 mL 1.1839 mL 2.3678 mL 5.9194 mL
    15 mM 0.1579 mL 0.7893 mL 1.5785 mL 3.9463 mL
    20 mM 0.1184 mL 0.5919 mL 1.1839 mL 2.9597 mL
    25 mM 0.0947 mL 0.4736 mL 0.9471 mL 2.3678 mL
    30 mM 0.0789 mL 0.3946 mL 0.7893 mL 1.9731 mL
    40 mM 0.0592 mL 0.2960 mL 0.5919 mL 1.4799 mL
    50 mM 0.0474 mL 0.2368 mL 0.4736 mL 1.1839 mL
    60 mM 0.0395 mL 0.1973 mL 0.3946 mL 0.9866 mL
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    Mangiferin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mangiferin4773-96-0NF-κBKeap1-Nrf2ApoptosisNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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