2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Methylcobalamin

メチルコバラミン  (Synonyms: Methylcobalamin; Mecobalamin; CH3-B12)

製品番号: HY-B0586 純度: 99.02%
COA 取扱説明書 Technical Support

Methylcobalamin (CH3-B12), a cobalamin, is a form of vitamin B12.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 13422-55-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
100 mg $50 在庫あり
500 mg $110 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 5 publication(s) in Google Scholar

Other Forms of Methylcobalamin:

メチルコバラミン 関連抗体

Top Publications Citing Use of Products

    Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    MST measurements of the binding of 0.2 μM recombinant caspase-3, GSDME, GSDMD with Methylcobalamin (MeCbl) or positive control. Methylcobalamin (MeCbl) specifically bound to GSDME but not to caspase-3 or GSDMD.

    Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    Representative immunoblot of GSDME pretreated with inhibitors as indicated, and then incubated with recombinant active caspase-3 for GSDME activation analysis. GSDME cleavage by caspase-3 was potently disrupted by Methylcobalamin (MeCbl) (1 μM; 30 min).

    Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    Changes in HepG2 cell morphology were observed with a microscope. Methylcobalamin (MeCbl) (20 μM; 2h) obviously blocked the appearance of pyroptotic bubbles (red arrow).

    Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    SYTOX fluorescence probe was used to investigate the protective effects of Methylcobalamin (MeCbl) (20 μM) on DCA-induced systemic and liver injury. The liver region was indicated with a red or yellow circle. Methylcobalamin (MeCbl) was effective against DCA-induced liver damage of zebrafish.

    Methylcobalamin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 31;16(1):1233.  [Abstract]

    Methylcobalamin (MeCbl) (10 mg/kg; i.g.; once daily) treatment drastically reduced Serum transaminase ALT/AST activities, IL-1α and IL-1β in wild-type six-week-old C57BL/6J male mice.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Methylcobalamin (CH3-B12), a cobalamin, is a form of vitamin B12.

    IC50 & Target

    Human Endogenous Metabolite

     

    体外実験

    Methylcobalamin (CH3-B12) is a cobalamin, a form of vitamin B12. It differs from cyanocobalamin in that the cyanide is replaced by a methyl group. Methylcobalamin (CH3-B12) features an octahedral cobalt(III) centre. Methylcobalamin (CH3-B12) can be obtained as bright red crystals. From the perspective of coordination chemistry, Methylcobalamin (CH3-B12) is notable as a rare example of a compound that contains metal-alkyl bonds. Methylcobalamin (CH3-B12) is equivalent physiologically to vitamin B12, and can be used to prevent or treat pathology arising from a lack of vitamin B12 (vitamin B12 deficiency), such as pernicious anemia. Methylcobalamin (CH3-B12) is also used in the treatment of peripheral neuropathy, diabetic neuropathy, and as a preliminary treatment for amyotrophic lateral sclerosis. From Wikipedia.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    メチルコバラミン 関連抗体
    臨床実験
    分子量

    1344.38

    分子式

    C63H91CoN13O14P
    CAS 番号
    Appearance

    Solid

    Color

    Brown to red

    SMILES

    C[C@]1([C@](C)2CC(N)=O)[N]([Co+3]([N](C([C@](C)3CC(N)=O)=C4C)=C5[C@H]3CCC(N)=O)([N](C(C(C)6C)=C5)=C([C@H]6CCC(N)=O)C(C)=C7[C@@]8(CCC(NC[C@H]9C)=O)C)([N](C%10=C%11)=CN([C@@H](O[C@@H]%12CO)[C@]([C@@H]%12O[P]([O-])(O9)=O)([H])O)C%10=CC(C)=C%11C)[N-]7[C@]1([H])[C@@H]8CC(N)=O)=C4[C@H]2CCC(N)=O.[CH3-]
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (37.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (3.72 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7438 mL 3.7192 mL 7.4384 mL
    5 mM 0.1488 mL 0.7438 mL 1.4877 mL
    10 mM 0.0744 mL 0.3719 mL 0.7438 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (1.86 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 18.75 mg/mL (13.95 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 100.00%

    COA (276 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.7438 mL 3.7192 mL 7.4384 mL 18.5959 mL
    DMSO 5 mM 0.1488 mL 0.7438 mL 1.4877 mL 3.7192 mL
    10 mM 0.0744 mL 0.3719 mL 0.7438 mL 1.8596 mL
    15 mM 0.0496 mL 0.2479 mL 0.4959 mL 1.2397 mL
    20 mM 0.0372 mL 0.1860 mL 0.3719 mL 0.9298 mL
    25 mM 0.0298 mL 0.1488 mL 0.2975 mL 0.7438 mL
    30 mM 0.0248 mL 0.1240 mL 0.2479 mL 0.6199 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Methylcobalamin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methylcobalamin13422-55-4Mecobalamin CH3-B12Endogenous MetaboliteInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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