2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Pilocarpine nitrate

Pilocarpine (nitrate)  (Synonyms: ピロカルピン 硝酸塩)

製品番号: HY-B1006 純度: 99.83%
COA 取扱説明書 Technical Support

Pilocarpine nitrate is a potent M3-type muscarinic acetylcholine receptor (M3 muscarinic receptor) agonist.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 148-72-1

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
50 mg $30 在庫あり
100 mg $50 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 41 publication(s) in Google Scholar

Other Forms of Pilocarpine nitrate:

Pilocarpine (nitrate) 関連抗体

Top Publications Citing Use of Products

顧客検証

In Vivo Efficacy Study
Histological Imaging/Staining
WB
IHC
IF

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332.  [Abstract]

    Seizure scores across different groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332.  [Abstract]

    Survival rate analysis among groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332.  [Abstract]

    Immunofluorescence staining of CD86 (pro-inflammatory) and CD206 (anti-inflammatory) expression in microglia within the hippocampus across groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635.  [Abstract]

    Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635.  [Abstract]

    Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635.  [Abstract]

    Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Apr 30;42(5):112456.  [Abstract]

    Latency before the first seizure of PTZ and Pilocarpine ((300 mg/kg; i.p.)-induced seizure tests in RACK1 fl/fl or RACK1 cKO mice.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Immunity. 2022 Aug 9;55(8):1466-1482.e9.  [Abstract]

    The effects of tamoxifen treatment and microglial PDGFB ablation on pilocarpine (100 mg/kg, ip, once every 20 minutes)-induced epilepsy were investigated by implanting telemetry probes into PDGFBNull-MG mice and analyzing their heart rate (HR) and heart rate variability (HRV) before and 3 or 5 weeks after tamoxifen treatment.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2022 Oct:217:102335.  [Abstract]

    The percentages of Foxp3+ Tregs among CD4+ T cells significantly increased in the hippocampal parenchyma of Pilocarpine(280-300 mg/kg; i.p.)-induced SE mice compared with those in the control group.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2022 Oct:217:102335.  [Abstract]

    GFAP immunostaining in the CA1, CA3, and DG regions of the hippocampi of vehicle-treated control mice, PC61-treated control mice, vehicle-treated Pilocarpine (280-300 mg/kg; i.p.) mice, and PC61-treated Pilocarpine mice.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cell Mol Neurobiol. 2022 Apr;42(3):777-790.  [Abstract]

    Pilocarpine (325 mg/kg, ip, single dose)-induced status epilepticus (SE) reduced the level of ALOXE3 protein in the hippocampus of mice, but did not reduce it in the temporal lobe cortex.

    Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cell Mol Neurobiol. 2022 Apr;42(3):777-790.  [Abstract]

    Pilocarpine (325 mg/kg, ip, single dose)-induced status epilepticus (SE) increases AA levels in the hippocampus of mice.

    mAChR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Pilocarpine nitrate is a potent M3-type muscarinic acetylcholine receptor (M3 muscarinic receptor) agonist.

    IC50 & Target

    mAChR3

     

    体外実験

    To evaluate the cytotoxicity of Pilocarpine, the morphology and viability of human corneal stromal (HCS) cells are examined by light microscopy and MTT assay, respectively. Morphological observations show that HCS cells exposed to Pilocarpine at a concentration from 0.625 to 20 g/L exhibit dose- and time-dependent proliferation retardation and morphological abnormality such as cellular shrinkage, cytoplasmic vacuolation, detachment from culture matrix, and eventually death, while no obvious difference is observed between those exposed to Pilocarpine below the concentration of 0.625 g/L and controls. Results of MTT assay reveal that the cell viability of HCS cells decrease with time and concentration after exposing to Pilocarpine above the concentration of 0.625 g/L (P<0.01 or 0.05), while that of HCS cells treated with Pilocarpine below the concentration of 0.625 g/L show no significant difference to controls[2]. The partial muscarinic agonist, Pilocarpine, evokes concentration-dependent relaxation with an EC50 of 2.4 mM in isolated segments of rat tail artery that were constricted with Penylephrine (10 to 200 nM)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pilocarpine (nitrate) 関連抗体
    体内実験

    The Pilocarpine-induced saliva secretion of the control rats (CN) and exercised (EX) rats is examined. A significantly greater amount of saliva is induced by Pilocarpine in the EX rats than in the CN rats (P<0.01). Conversely, the Na+ concentration in the saliva of the EX rats is significantly lower than that of the CN rats (P<0.05)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    271.27

    分子式

    C11H17N3O5
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [O-][N+](O)=O.O=C1OC[C@H](CC2=CN=CN2C)[C@@H]1CC
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *The compound is unstable in solutions, freshly prepared is recommended.
    溶剤 & 溶解度
    体外: 

    DMSO : 250 mg/mL (921.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (368.64 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6864 mL 18.4318 mL 36.8636 mL
    5 mM 0.7373 mL 3.6864 mL 7.3727 mL
    10 mM 0.3686 mL 1.8432 mL 3.6864 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション
    参考文献
    細胞実験
    [2]

    Cell viability is determined by MTT assay. Briefly, HCS cells are inoculated into a 96-well culture plate (Nunc) at a density of 1×104 cells/100 µL/well, and are cultured and treated. At a 4h interval, the Pilocarpine (0.625 to 20 g/L)-containing medium is replaced entirely with 100 µL serum-free DMEM/F12 medium containing 1.0 g/L MTT, and the cells are incubated at 37°C in the dark for 4h. After the MTT-containing medium is discarded with caution, 150 µL DMSO is added to dissolve the produced formazan crystals at 37°C in the dark for 15 min, and the absorbance at 490 nm is measured with a Multiskan GO microplate reader[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [1]

    Rats[1]
    Male, 10-week-old Wistar rats are assigned to one of two groups, exercise (EX, n=6) and control (CN, n=6). The EX rats are kept for 40 days in cages with a running wheel (SN-451), allowing them to undertake voluntary exercise, while the CN rats are kept in cages with the running wheel locked. On the 40th day, Pilocarpine-induced saliva is measured as follows. Briefly, the rats are anesthetized, preweighed cotton was placed in their mouths sublingually, and Pilocarpine (0.5 mg/kg) is intraperitoneally injected to induce saliva secretion. Each cotton ball is then changed every 10 min for 1 h. The collected cotton balls are weighed again, and the mass of saliva secreted is calculated by subtracting the initial from the final weight.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.6864 mL 18.4318 mL 36.8636 mL 92.1591 mL
    5 mM 0.7373 mL 3.6864 mL 7.3727 mL 18.4318 mL
    10 mM 0.3686 mL 1.8432 mL 3.6864 mL 9.2159 mL
    15 mM 0.2458 mL 1.2288 mL 2.4576 mL 6.1439 mL
    20 mM 0.1843 mL 0.9216 mL 1.8432 mL 4.6080 mL
    25 mM 0.1475 mL 0.7373 mL 1.4745 mL 3.6864 mL
    30 mM 0.1229 mL 0.6144 mL 1.2288 mL 3.0720 mL
    40 mM 0.0922 mL 0.4608 mL 0.9216 mL 2.3040 mL
    50 mM 0.0737 mL 0.3686 mL 0.7373 mL 1.8432 mL
    60 mM 0.0614 mL 0.3072 mL 0.6144 mL 1.5360 mL
    80 mM 0.0461 mL 0.2304 mL 0.4608 mL 1.1520 mL
    100 mM 0.0369 mL 0.1843 mL 0.3686 mL 0.9216 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Pilocarpine nitrate Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pilocarpine148-72-1mAChRMuscarinic acetylcholine receptorInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Pilocarpine nitrate
    製品番号:
    HY-B1006
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.