2. Apoptosis
  3. Bcl-2 Family
  4. Sabutoclax

Sabutoclax is a potent and effective Bcl-2 Family (Bcl-2, Bcl-XL, Mcl-1, Bfl-1) inhibitor with IC50s of 0.32 μM, 0.31 μM, 0.20 μM, and 0.62 μM, respectively. Sabutoclax increases Bax, Bim, PUMA and survivin expression.

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Sabutoclax

Sabutoclax 화학구조

CAS No. : 1228108-65-3

사이즈 가격 재고 수량
5 mg 해외재고보유
10 mg 해외재고보유
50 mg   견적 받기  
100 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of Sabutoclax:

Sabutoclax Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Sabutoclax

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Sabutoclax is a potent and effective Bcl-2 Family (Bcl-2, Bcl-XL, Mcl-1, Bfl-1) inhibitor with IC50s of 0.32 μM, 0.31 μM, 0.20 μM, and 0.62 μM, respectively. Sabutoclax increases Bax, Bim, PUMA and survivin expression[1][2].

IC50 & Target[1]

Bcl-xL

0.31 μM (IC50)

BCL2

0.32 μM (IC50)

Mcl-1

0.2 μM (IC50)

Bfl-1

0.62 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
NCI-H1299 EC50
3.2 μM
Compound: 8r
Cytotoxicity against human H1299 cells assessed as cell viability after 72 hrs by ATP-LITE assay
Cytotoxicity against human H1299 cells assessed as cell viability after 72 hrs by ATP-LITE assay
[PMID: 19555126]
NCI-H460 EC50
0.33 μM
Compound: 8r
Cytotoxicity against human H460 cells assessed as cell viability after 72 hrs by ATP-LITE assay
Cytotoxicity against human H460 cells assessed as cell viability after 72 hrs by ATP-LITE assay
[PMID: 19555126]
NCI-H460 EC50
0.42 μM
Compound: (R,-,R)-11, atropisoer
Cytotoxicity against human H460 cells after 3 days by ATP-LITE assay
Cytotoxicity against human H460 cells after 3 days by ATP-LITE assay
[PMID: 20443627]
NCI-H460 EC50
0.47 μM
Compound: rac-4
Cytotoxicity against human H460 cells after 3 days by ATP-LITE assay
Cytotoxicity against human H460 cells after 3 days by ATP-LITE assay
[PMID: 20443627]
NCI-H460 EC50
0.78 μM
Compound: (R, +, R)-12
Cytotoxicity against human H460 cells after 3 days by ATP-LITE assay
Cytotoxicity against human H460 cells after 3 days by ATP-LITE assay
[PMID: 20443627]
PC-3 EC50
0.13 μM
Compound: (R,-,R)-11, atropisoer
Cytotoxicity against human PC3 cells after 3 days by ATP-LITE assay
Cytotoxicity against human PC3 cells after 3 days by ATP-LITE assay
[PMID: 20443627]
PC-3 EC50
2.45 μM
Compound: rac-4
Cytotoxicity against human PC3 cells after 3 days by ATP-LITE assay
Cytotoxicity against human PC3 cells after 3 days by ATP-LITE assay
[PMID: 20443627]
PC-3 EC50
4.64 μM
Compound: (R, +, R)-12
Cytotoxicity against human PC3 cells after 3 days by ATP-LITE assay
Cytotoxicity against human PC3 cells after 3 days by ATP-LITE assay
[PMID: 20443627]
In Vitro

Sabutoclax (0.001-10 μM; 72 h) potentially inhibits cell growth of human prostate cancer, lung cancer cell line[1].
Sabutoclax (0.01 μM-1 μM; 24-48 h) potentially induces cell apoptosis in human diffuse large B-cell lymphoma cell line[1].
Sabutoclax (0 μM-15 μM; 48 h) uptrgulates the level of pro-apoptotic proteins in chemoresistent cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sabutoclax Related Antibodies

Cell Viability Assay[1]

Cell Line: PC-3 and H460
Concentration: 0.001 μM, 0.01 μM, 0.1 μM, 1 μM, 10 μM (PC-3), 0.05 μM, 0.1 μM, 1 μM, 5 μM, 10 μM (H460)
Incubation Time: 72 h
Result: Showed effectively repressing cell growth in a dose-dependent manner.

Apoptosis Analysis[1]

Cell Line: BP3 cell line
Concentration: 0.01 μM, 0.03 μM, 0.1 μM, 1 μM
Incubation Time: 24-48 h
Result: Effectively induced apoptosis in a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: MCF-7/A02, and CALDOX cells
Concentration: 0 μM, 7.5 μM, 15 μM (MCF-7/A02), 0 μM, 5 μM, 10 μM (CALDOX)
Incubation Time: 48 h
Result: Showed increasing proteins level of Bax, Bim, PUMA and Survivin in a dose-dependent manner.
In Vivo

Sabutoclax (1-5 mg/kg; i.p.; every two days in 18 D) reduces tumor growth in M2182-bearing athymic nude mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: athymic nude mice with tumor xenografts from M2182 cells in s.c.
Dosage: 1 mg/kg, 3 mg/kg, 5 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Showed ihibiting tumor growth to about 60% of the tumor volume.
분자량

700.78

화학식

C42H40N2O8
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=C(C1=C(O)C(O)=CC2=C(O)[C@@]([C@@]3=C(C)C=C4C(C(NC[C@@H](C5=CC=CC=C5)C)=O)=C(O)C(O)=CC4=C3O)=C(C)C=C12)NC[C@@H](C6=CC=CC=C6)C
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

DMSO : 36.67 mg/mL (52.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4270 mL 7.1349 mL 14.2698 mL
5 mM 0.2854 mL 1.4270 mL 2.8540 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.75 mg/mL (3.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서

Purity: 99.80%

SDS (393 KB)

COA (250 KB) HNMR (269 KB) RP-HPLC (258 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4270 mL 7.1349 mL 14.2698 mL 35.6745 mL
5 mM 0.2854 mL 1.4270 mL 2.8540 mL 7.1349 mL
10 mM 0.1427 mL 0.7135 mL 1.4270 mL 3.5675 mL
15 mM 0.0951 mL 0.4757 mL 0.9513 mL 2.3783 mL
20 mM 0.0713 mL 0.3567 mL 0.7135 mL 1.7837 mL
25 mM 0.0571 mL 0.2854 mL 0.5708 mL 1.4270 mL
30 mM 0.0476 mL 0.2378 mL 0.4757 mL 1.1892 mL
40 mM 0.0357 mL 0.1784 mL 0.3567 mL 0.8919 mL
50 mM 0.0285 mL 0.1427 mL 0.2854 mL 0.7135 mL
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Sabutoclax Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sabutoclax1228108-65-3BI-97C1Bcl-2 FamilyBcl-1 Family InhibitorCancerInhibitorinhibitorinhibit

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상품명:
Sabutoclax
Cat. No.:
HY-15191
수량:
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SAFETY DATA SHEET (SDS)

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