TPPB

Name: TPPB
Brand: MedChemExpress
SKU: HY-12359
Rating: 4.5 (1 reviews)

Cat. No.: HY-12359 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

TPPB is a cell-permeable benzolactam-derived protein kinase C (PKC) activator with a K_i of 11.9 nM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 497259-23-1

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE TPPB

•Viruses. 2020 Jun 3;12(6):609. [Abstract]

ELISA

RT-PCR

Flow Cytometry

: TPPB purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 12;16(1):11092. [Abstract]; Western blotting analysis of RKIP expression in PA-stimulated HepG2 cells with MG-132 (10 μM; 12 h) treatment.

: TPPB purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 12;16(1):11092. [Abstract]; MG-132 (10 μM; 6 h) rescued the decreased expression of RKIP in HepG2 cells with ER stress inducer (tunicamycin, TM; thapsigargin, Tha).

: TPPB purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 12;16(1):11092. [Abstract]; MG-132 (10 μM; 12 h) rescued the decreased expression of RKIP^C133A protein in HEK293T cells.

: TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609. [Abstract]; Effect of TPPB (0.5 μM; 48 h) on the reactivation of latent HIV in U1 and ACH-2 cells.

: TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609. [Abstract]; TPPB (0.5 μM; 48 h) reactivated latent HIV in OM-10.1, ACH-2, and U1 cells, as measured using HIV RNA quantification.

: TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609. [Abstract]; Effect of TPPB (0.5 μM; 48 h) on the reactivation of latent HIV in OM-10.1 cells.

: TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609. [Abstract]; TPPB (0.5 μM; 24-48 h) induced gp120 expression in latently infected cells (OM-10.1 cells and ACH-2 cells).

: TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609. [Abstract]; Representative overlay and single peaks of CD4 expression of CD4+ T-cells treated with TPPB (0.5 μM; 24 h).

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

TPPB is a cell-permeable benzolactam-derived protein kinase C (PKC) activator with a K_i of 11.9 nM.

IC₅₀ & Target^[1]

PKC

11.9 nM (Ki)

In Vitro

By use of a cell line derived from an Alzheimer’s disease patient, significant enhancement of sAPPα secretion is achieved at 1 μM concentration for TPPB (Compound 5e)^[1]. TPPB has a role against Aβ_25-35-induced neurotoxicity in PC12 cells. TPPB at concentration of 1 μM could antagonize Aβ_25-35 induced cell damage. TPPB could increase the phosphorylation of Akt, PKC, MARCKS and MAPK, which are inhibited by Aβ_25-35 treatment. TPPB inhibits the activation of caspase-3 induced by Aβ_25-35^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TPPB is evaluated for induction of hyperplasia after topical application to the shaved backs of outbred Sencar mice and shows a modest response at 300 μg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

501.54

화학식

C₂₇H₃₀F₃N₃O₃

CAS No.

497259-23-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=CC=C(N(C)[C@@H](C(C)C)C(N[C@H](CO)C2)=O)C2=C1)/C=C/C=C/C3=CC=C(C(F)(F)F)C=C3

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 125 mg/mL (249.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9939 mL	9.9693 mL	19.9386 mL
5 mM	0.3988 mL	1.9939 mL	3.9877 mL
10 mM	0.1994 mL	0.9969 mL	1.9939 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.25 mg/mL (4.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.25 mg/mL (4.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.92%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (246 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Kozikowski AP, et al. New amide-bearing benzolactam-based protein kinase C modulators induce enhanced secretion of the amyloid precursor protein metabolite sAPPalpha. J Med Chem. 2003 Jan 30;46(3):364-73. [Content Brief]

[2]. Yang HQ, et al. Neuroprotective effects of new protein kinase C activator TPPB against Aβ25-35 induced neurotoxicity in PC12 cells. Neurochem Res. 2012 Oct;37(10):2213-21. [Content Brief]

Cell Assay ^[2]	24 h after plating 2×10⁴ pheochromocytoma PC12 cells in each well of a 96 well plate, cells are incubated with TPPB at a series of concentration (0.1, 0.5, 1, 5, 10, 20 μM). Twelve to 24 h later, the original media is replaced with media containing MTT at a final concentration of 0.5 g/L for 4 h. Cell viability is evaluated with MTT assays^[2]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
Animal Administration ^[1]	Mice: Mice are 7 weeks old at the beginning of the treatments and are in the resting phase of the hair cycle. TPPB is applied once or else are applied twice weekly for a total of four applications. Two animals are treated at each dose of compound, and 72 h after the last application, the animals are euthanized. Two portions of treated skin are removed from each animal, fixed in neutral buffered formalin, and stained with hematoxylin and eosin for histological analysis^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Kozikowski AP, et al. New amide-bearing benzolactam-based protein kinase C modulators induce enhanced secretion of the amyloid precursor protein metabolite sAPPalpha. J Med Chem. 2003 Jan 30;46(3):364-73. [Content Brief] [2]. Yang HQ, et al. Neuroprotective effects of new protein kinase C activator TPPB against Aβ25-35 induced neurotoxicity in PC12 cells. Neurochem Res. 2012 Oct;37(10):2213-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9939 mL	9.9693 mL	19.9386 mL	49.8465 mL
	5 mM	0.3988 mL	1.9939 mL	3.9877 mL	9.9693 mL
	10 mM	0.1994 mL	0.9969 mL	1.9939 mL	4.9846 mL
	15 mM	0.1329 mL	0.6646 mL	1.3292 mL	3.3231 mL
	20 mM	0.0997 mL	0.4985 mL	0.9969 mL	2.4923 mL
	25 mM	0.0798 mL	0.3988 mL	0.7975 mL	1.9939 mL
	30 mM	0.0665 mL	0.3323 mL	0.6646 mL	1.6615 mL
	40 mM	0.0498 mL	0.2492 mL	0.4985 mL	1.2462 mL
	50 mM	0.0399 mL	0.1994 mL	0.3988 mL	0.9969 mL
	60 mM	0.0332 mL	0.1662 mL	0.3323 mL	0.8308 mL
	80 mM	0.0249 mL	0.1246 mL	0.2492 mL	0.6231 mL
	100 mM	0.0199 mL	0.0997 mL	0.1994 mL	0.4985 mL

TPPB Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TPPB497259-23-1MARCKSPKCMyristoylated alanine-rich C kinase substrateProtein kinase CInhibitorinhibitorinhibit

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TPPB

고객리뷰

TPPB Related Antibodies

1 Publications Citing Use of MCE TPPB

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKC Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

TPPB Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

TPPB Related Classifications

Keywords:

최근 본 상품:

고객리뷰

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SAFETY DATA SHEET (SDS)

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