2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-137853
    4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside
    		99.95%
    4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside is a fluorogenic substrate for N-acetyl-β-D-glucosaminidase.
    4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside
  • HY-D1539
    Cyanine 5.5 azide
    		98.20%
    Cyanine 5.5 azide (CY 5.5 azide) is a potent fluorescent dye. Cyanine 5.5 azide can label DNA. Cyanine 5.5 azide can be used for NIR live organism imaging. (λex=684 nm, λem=710 nm). Cyanine 5.5 azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cyanine 5.5 azide
  • HY-D2173
    AF488 azide
    		98.83%
    AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 azide
  • HY-NP062
    Bovine Serum Albumin-Cy5.5
    Bovine Serum Albumin-Cy5.5 is Cy5.5-labeled Bovine Serum Albumin (BSA) (HY-D0842). Bovine Serum Albumin-Cy5.5 can be used in fields such as bioimaging, molecular tracing, drug delivery research, and in vitro and in vivo fluorescence detection (Ex/Em = 680/710 nm).
    Bovine Serum Albumin-Cy5.5
  • HY-D2452
    Cy3-Cisplatin
    Cy3-Cisplatin is a Cyanine 3 fluorescent dye CY3 (HY-D0822) labled Cisplatin (HY-17394). Cy3-Cisplatin can be used for drug delivery, fluorescent labeling, cell imaging and tracking.
    Cy3-Cisplatin
  • HY-D1341
    Coumberone
    		99.73%
    Coumberone is a metabolic fluorogenic probe, and isoform-selective substrate for all AKR1C isoforms. Coumberone can be reduced by all four members of the AKR1C family to its fluorescent alcohol coumberol. Coumberone can be used for the research of AKR1C.
    Coumberone
  • HY-W088075
    Acriflavine hydrochloride
    		99.4%
    Acriflavine (Acriflavinium chloride) hydrochloride is a fluorescent acridine dye that can be used to label nucleic acid. Acriflavine hydrochloride is an antiseptic agent. Acriflavine hydrochloride is a potent HIF-1 inhibitor that prevents the dimerization of HIF-1α and HIF-1β subunits. Acriflavine hydrochloride inhibits the interaction between monocarboxylate transporter 4 (MCT4) and Basigin. Acriflavine hydrochloride is used in cancer research, such as breast cancer, brain tumor and chronic myeloid leukemia. Acriflavine hydrochloride is a potent papain-like protease (PLpro) inhibitor, which inhibits SARS-CoV-2.
    Acriflavine hydrochloride
  • HY-P3722A
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA
    		99.54%
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA is a fluorescent substrate peptide that can be used to detect ADAM9, ADAM10, and tumor necrosis factor-α converting enzyme (TACE/ADAM17). Mca-PLAQAV-Dpa-RSSSR-NH2 TFA is a fluorescence resonance energy transfer-based substrate, and its activity can be determined by changes in fluorescence intensity upon cleavage (Ex = 320 nm ; Em = 405 nm).
    Mca-PLAQAV-Dpa-RSSSR-NH2 TFA
  • HY-D1815
    Allophycocyanin
    Allophycocyanin (APC Dye) is a light-harvesting protein, containing strongly coupled dimers of chromophores. Allophycocyanin serve as a minimal system to examine photosynthetic energy transfer. Allophycocyanin strongly absorbs far-red light (FRL), and expresses during acclimation to low light, likely associates with chlorophyll a-containing photosystem I.
    Allophycocyanin
  • HY-D1849
    Cy3B NHS ester
    Cy3B NHS ester is a fluorescent dye compound that is commonly used in biomarking and fluorescent labeling experiments, especially for labeling biomolecules containing amino functional groups (amine groups), such as proteins, antibodies or peptides.
    Cy3B NHS ester
  • HY-D1191
    SYBR green I chloride
    		98.09%
    SYBR Green I chloride is a highly sensitive fluorescent nucleic acid dye that binds specifically to the minor groove of double-stranded DNA or intercalates between base pairs. SYBR Green I chloride exhibits weak fluorescence in the unbound state but emits bright fluorescence upon binding, and it preferentially binds to large-fragment DNA and DNA with high G+C content. SYBR Green I chloride is suitable for real-time PCR technology; its fluorescence intensity correlates with the amount and size of amplification products, enabling accurate quantification of gene expression and discrimination of amplicons via melting curve analysis without additional post-processing. SYBR Green I chloride is widely used in preclinical in vitro nucleic acid detection.
    SYBR green I chloride
  • HY-131008
    Fluorescein tyramide
    		≥99.0%
    Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications.
    Fluorescein tyramide
  • HY-DY1011
    PKH 26 (solution)
    PKH 26 (solution) is a red fluorescent dye, PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm) , which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies.
    Solvent and concentration: DMSO: 5 mM
    PKH 26 (solution)
  • HY-110213
    BODIPY 630/650X
    BODIPY 630/650X is a red fluorescent dye that targets amine groups. BODIPY 630/650X displays excitation/emission maxima of 630/650 nm, respectively. BODIPY 630/650X can be used to covalently label proteins, antibodies, or other amine-containing biomolecules for detection and imaging.
    BODIPY 630/650X
  • HY-123275B
    H-D-Phe-Pip-Arg-pNA acetate
    		99.81%
    H-D-Phe-Pip-Arg-pNA (S-2238) acetate, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA acetate is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA acetate is sensitive, accurate, and easy to perform.
    H-D-Phe-Pip-Arg-pNA acetate
  • HY-129042
    Cibacron Blue 3G-A
    Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a Ki value of 1.2 uM.
    Cibacron Blue 3G-A
  • HY-D2119
    CQ-Lyso
    CQ-Lyso is a lysosome-targeting chromenoquinoline. CQ-Lyso is a fluorescent probe for measuring the pH of lysosomes in living cells using only single wavelength excitation. CQ-Lyso can visualize the dynamic pH changes in lysosomes.
    CQ-Lyso
  • HY-156404
    PM-1
    		99.04%
    PM-1, a derivative of Thioflavin-T (ThT; HY-D0218), is a small but highly specific plasma membrane (PM) fluorescent dye for specific and long-time membrane imaging of living and fixed cells. PM-1 is embedded directly into the cell membrane and exhibits a very long retention time on the plasma membrane with a half-life of approximately 15 h. PM-1 can be used in combination with protein labeling probes to study ectodomain shedding and endocytosis processes of cell surface proteins.
    PM-1
  • HY-D0916
    Thiazole orange dimer YOYO 1
    Thiazole orange dimer YOYO 1 (YOYO 1) is a cell-impermeable cyanine dimer with no inherent fluorescence, and its fluorescence intensity increases significantly upon binding to double-stranded DNA (dsDNA). Thiazole orange dimer YOYO 1 can serve as a nuclear counterstain or a dead cell indicator (Ex/Em = 505/512 nm).
    Thiazole orange dimer YOYO 1
  • HY-158082H
    TRITC-dextran, MW 2000000
    TRITC-dextran, MW 2000000 (Tetramethyl rhodamine isothiocyanate glucan, MW 2000000) is a fluorescent dye, with the molecular weight of 2000 kD. TRITC-dextran, MW 2000000 exhibits an excitation wavelength of 555 nm. TRITC-dextran, MW 2000000 is follicular and vessel penetrate, which is dependent on the molecular weight. TRITC-dextran, MW 2000000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 2000000
Cat. No. Product Name / Synonyms Application Reactivity