BODIPY 630/650X 

BODIPY 630/650X is a red fluorescent dye that targets amine groups. BODIPY 630/650X displays excitation/emission maxima of 630/650 nm, respectively. BODIPY 630/650X can be used to covalently label proteins, antibodies, or other amine-containing biomolecules for detection and imaging^[1].

Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
BODIPY 630/650X has advantages in live cell imaging, and its fluorescence signal can be clearly distinguished from cellular autofluorescence background^[1].
Live cell imaging protocol^[2][3]:
1. Preparation of stock solution
Dissolve BODIPY 630/650-X in DMSO to prepare a stock solution at a concentration of 1 mg/mL, and store at 4°C protected from light.
2. Preparation of working solution
Dilute the BODIPY 630/650-X stock solution with PBS to obtain a final working concentration of 5 μg/mL. The working solution should be freshly prepared and protected from light until use.
3. Cell staining
3.1 Wash cells twice with pre-warmed PBS to remove serum components.
3.2 Incubate cells with 5 μg/mL BODIPY 630/650-X working solution at room temperature for 25-30 min.
4. Washing
Wash cells 3-4 times with complete medium containing 5-10% serum or 1% BSA (or PBS) to quench unbound dye. Finally, wash once with live cell imaging buffer.
5. Imaging
Immediately acquire images using a confocal microscope or high-content imaging system. Excite with a HeNe laser (wavelength 633 nm) and collect emission using a ≥ 650 nm long-pass filter.
Note: The optimal dye concentration and incubation time may vary depending on cell type and application.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

660.50

C₃₃H₃₁BF₂N₄O₆S

380367-48-6

Solid

Dark purple to reddish brown

650

630

O=C(CCCCCNC(COC1=CC=C(/C=C/C2=CC=C3C=C4C=CC(C5=CC=CS5)=[N]4[B+3]([F-])([N-]23)[F-])C=C1)=O)ON6C(CCC6=O)=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Casella BM, et al. Design, Synthesis, and Application of Fluorescent Ligands Targeting the Intracellular Allosteric Binding Site of the CXC Chemokine Receptor 2. J Med Chem. 2023 Sep 28;66(18):12911-12930.  [Content Brief]

  [2]. Bedner E, et al. Multiparameter analysis of progeny of individual cells by laser scanning cytometry. Cytometry. 2000 Aug 1;40(4):271-9.  [Content Brief]

  [3]. Smolewski P, et al. Bivariate analysis of cellular DNA versus RNA content by laser scanning cytometry using the product of signal subtraction (differential fluorescence) as a separate parameter. Cytometry. 2001 Sep 1;45(1):73-8.  [Content Brief]