BODIPY 630/650X

Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
BODIPY 630/650X has advantages in live cell imaging, and its fluorescence signal can be clearly distinguished from cellular autofluorescence background^[1].
Live cell imaging protocol^[2][3]:
1. Preparation of stock solution
Dissolve BODIPY 630/650-X in DMSO to prepare a stock solution at a concentration of 1 mg/mL, and store at 4°C protected from light.
2. Preparation of working solution
Dilute the BODIPY 630/650-X stock solution with PBS to obtain a final working concentration of 5 μg/mL. The working solution should be freshly prepared and protected from light until use.
3. Cell staining
3.1 Wash cells twice with pre-warmed PBS to remove serum components.
3.2 Incubate cells with 5 μg/mL BODIPY 630/650-X working solution at room temperature for 25-30 min.
4. Washing
Wash cells 3-4 times with complete medium containing 5-10% serum or 1% BSA (or PBS) to quench unbound dye. Finally, wash once with live cell imaging buffer.
5. Imaging
Immediately acquire images using a confocal microscope or high-content imaging system. Excite with a HeNe laser (wavelength 633 nm) and collect emission using a ≥ 650 nm long-pass filter.
Note: The optimal dye concentration and incubation time may vary depending on cell type and application.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.