Cibacron Blue 3G-A

Name: Cibacron Blue 3G-A
Brand: MedChemExpress
SKU: HY-129042
Rating: 4.5 (1 reviews)

Cat. No.: HY-129042 Purity: 72%: Data Sheet
COA

SDS
Handling Instructions
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Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a K_i value of 1.2 uM.

For research use only. We do not sell to patients.

Cibacron Blue 3G-A Chemical Structure

CAS No. : 84166-13-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Cibacron Blue 3G-A

•Biochem Biophys Res Commun. 2023 Jul 12:665:64-70.

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View All Isoforms

Metallo-β-lactamase (MBL) Serine β-lactamase (SBL)

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a K_i value of 1.2 uM^[1].

IC₅₀ & Target

IC50: anthraquinone dye^[1]

In Vitro

Cibacron Blue 3G-A is a structural analogy between the dye and NADH, it interacts with (di)nucleotide-dependent enzymes and can be a standard tool for studying their active sites^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

774.16

Formula

C₂₉H₂₀ClN₇O₁₁S₃

CAS No.

84166-13-2

Appearance

Solid

Color

Brown to black

SMILES

O=S(C(C(N)=C1C2=O)=CC(NC3=CC=C(NC4=NC(Cl)=NC(NC5=CC=CC=C5S(=O)(O)=O)=N4)C(S(=O)(O)=O)=C3)=C1C(C6=C2C=CC=C6)=O)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 58.33 mg/mL (75.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M NaOH : 10 mg/mL (12.92 mM; ultrasonic and adjust pH to 12 with NaOH)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2917 mL	6.4586 mL	12.9172 mL
5 mM	0.2583 mL	1.2917 mL	2.5834 mL
10 mM	0.1292 mL	0.6459 mL	1.2917 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 72%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (260 KB)

Handling Instructions (2659 KB)

References

[1]. Monaghan C, et al. The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase.Biochem J. 1982 Aug 1;205(2):413-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

1M NaOH / DMSO	1 mM	1.2917 mL	6.4586 mL	12.9172 mL	32.2931 mL
	5 mM	0.2583 mL	1.2917 mL	2.5834 mL	6.4586 mL
	10 mM	0.1292 mL	0.6459 mL	1.2917 mL	3.2293 mL
DMSO	15 mM	0.0861 mL	0.4306 mL	0.8611 mL	2.1529 mL
	20 mM	0.0646 mL	0.3229 mL	0.6459 mL	1.6147 mL
	25 mM	0.0517 mL	0.2583 mL	0.5167 mL	1.2917 mL
	30 mM	0.0431 mL	0.2153 mL	0.4306 mL	1.0764 mL
	40 mM	0.0323 mL	0.1615 mL	0.3229 mL	0.8073 mL
	50 mM	0.0258 mL	0.1292 mL	0.2583 mL	0.6459 mL
	60 mM	0.0215 mL	0.1076 mL	0.2153 mL	0.5382 mL