Thiazole orange dimer YOYO 1

Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs.)
1. Prepare Working Solution
1.1 Dilute the stock solution with pre-warmed Hanks' solution containing 20 mM Hepes buffer (i.e., HH buffer), serum-free cell culture medium or PBS to prepare a working solution with a concentration of 1-10 μM.
Note: Adjust the concentration of the working solution according to actual conditions, and prepare it fresh before use.
2. Cell Staining
2.1 Culture the cells
2.2 Remove the cell culture medium and fix the cells.
2.3 Add 1-10 μM dye working solution, gently shake to completely cover the cells, and incubate for 15-60 minutes.
2.4 Aspirate the dye working solution, add HH buffer or other buffer to wash 2-3 times, 5 minutes each time.
2.5 Observe using a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.