2. Others
  3. Fluorescent Dye
  4. AF488 azide

AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.

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AF488 azide

AF488 azide Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg   Get quote  
50 mg   Get quote  

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AF488 azide Related Antibodies

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  • Purity & Documentation

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Description

AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively[1][2].

In Vitro

AF488 azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AF488 azide Related Antibodies
Molecular Weight

692.76

Formula

C24H18K2N6O10S2
Appearance

Solid

Color

Brown to red

SMILES

O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O[K])=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(NCCCN=[N+]=[N-])=O)=C1)O[K]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (60.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4435 mL 7.2175 mL 14.4350 mL
5 mM 0.2887 mL 1.4435 mL 2.8870 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.83%

SDS (394 KB)

COA (253 KB) HNMR (132 KB) RP-HPLC (262 KB) MS (123 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4435 mL 7.2175 mL 14.4350 mL 36.0875 mL
5 mM 0.2887 mL 1.4435 mL 2.8870 mL 7.2175 mL
10 mM 0.1444 mL 0.7218 mL 1.4435 mL 3.6088 mL
15 mM 0.0962 mL 0.4812 mL 0.9623 mL 2.4058 mL
20 mM 0.0722 mL 0.3609 mL 0.7218 mL 1.8044 mL
25 mM 0.0577 mL 0.2887 mL 0.5774 mL 1.4435 mL
30 mM 0.0481 mL 0.2406 mL 0.4812 mL 1.2029 mL
40 mM 0.0361 mL 0.1804 mL 0.3609 mL 0.9022 mL
50 mM 0.0289 mL 0.1444 mL 0.2887 mL 0.7218 mL
60 mM 0.0241 mL 0.1203 mL 0.2406 mL 0.6015 mL
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AF488 azide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AF488 azideFluorescent DyeInhibitorinhibitorinhibit

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