AF488 azide
Based on 1 Customer Validation
AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. AF488 azide exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
For research use only. We do not sell to patients.
- Purity: 98.83%
- Formula: C24H18K2N6O10S2
- Molecular Weight:692.76
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
AF488 azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 692.76
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Formula C24H18K2N6O10S2
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Color Brown to red
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SMILES
O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O[K])=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(NCCCN=[N+]=[N-])=O)=C1)O[K]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 41.67 mg/mL (60.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4435 mL | 7.2175 mL | 14.4350 mL | 36.0875 mL |
| 5 mM | 0.2887 mL | 1.4435 mL | 2.8870 mL | 7.2175 mL | |
| 10 mM | 0.1444 mL | 0.7218 mL | 1.4435 mL | 3.6088 mL | |
| 15 mM | 0.0962 mL | 0.4812 mL | 0.9623 mL | 2.4058 mL | |
| 20 mM | 0.0722 mL | 0.3609 mL | 0.7218 mL | 1.8044 mL | |
| 25 mM | 0.0577 mL | 0.2887 mL | 0.5774 mL | 1.4435 mL | |
| 30 mM | 0.0481 mL | 0.2406 mL | 0.4812 mL | 1.2029 mL | |
| 40 mM | 0.0361 mL | 0.1804 mL | 0.3609 mL | 0.9022 mL | |
| 50 mM | 0.0289 mL | 0.1444 mL | 0.2887 mL | 0.7218 mL | |
| 60 mM | 0.0241 mL | 0.1203 mL | 0.2406 mL | 0.6015 mL |
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