H-D-Phe-Pip-Arg-pNA acetate
Based on 1 publication(s) in Google Scholar
H-D-Phe-Pip-Arg-pNA acetate (S-2238 acetate) is a specific chromogenic peptide substrate for thrombin (Thrombin). The amide bond of H-D-Phe-Pip-Arg-pNA acetate is rapidly hydrolyzed by thrombin, producing a characteristic yellow absorbance at 405 nm, and the change in absorbance is proportional to thrombin activity. H-D-Phe-Pip-Arg-pNA acetate can be used in biochemical detection-related studies.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 115388-96-0
- Formula: C29H40N8O7
- Molecular Weight:612.68
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) H-D-Phe-Pip-Arg-pNA acetate
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Biological Activity
H-D-Phe-Pip-Arg-pNA (S-2238) (compound 3) acetate enables a sensitive, accurate thrombin-based chromogenic assay for heparin in human plasma, with a minimum detectable heparin concentration of 0.005 U/mL in normal plasma; the performance of this assay at low heparin concentrations is improved when purified human antithrombin III is used[1].
H-D-Phe-Pip-Arg-pNA acetate is the most reactive substrate for plasmin F-III-2 and F-III-1 purified from Lumbricus rubellus[2].
H-D-Phe-Pip-Arg-pNA acetate can be acted upon by plasmin purified from Lumbricus rubellus; these plasmins retain full amidolytic activity towards this substrate after incubation at 37°C and pH 1-11 for 30 min; the amidolytic activity of plasmin towards this substrate is strongly inhibited by DFP, SBTI and aprotinin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 115388-96-0
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Appearance Solid
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Molecular Weight 612.68
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Formula C29H40N8O7
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Color White to yellow
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Synonyms
S-2238 acetate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Small
2026 Mar;22(13):e2500193. PMID: 40388659
Solvent & Solubility
DMSO : 100 mg/mL (163.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (163.22 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (560 KB)
- English - EN (560 KB)
- Français - FR (560 KB)
- Deutsch - DE (560 KB)
- Norwegian - NO (560 KB)
- Español - ES (560 KB)
- Swedish - SV (560 KB)
- Italian - IT (560 KB)
- Korean - KR (560 KB)
- Portuguese - PT (560 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.6322 mL | 8.1609 mL | 16.3217 mL | 40.8043 mL |
| 5 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL | 8.1609 mL | |
| 10 mM | 0.1632 mL | 0.8161 mL | 1.6322 mL | 4.0804 mL | |
| 15 mM | 0.1088 mL | 0.5441 mL | 1.0881 mL | 2.7203 mL | |
| 20 mM | 0.0816 mL | 0.4080 mL | 0.8161 mL | 2.0402 mL | |
| 25 mM | 0.0653 mL | 0.3264 mL | 0.6529 mL | 1.6322 mL | |
| 30 mM | 0.0544 mL | 0.2720 mL | 0.5441 mL | 1.3601 mL | |
| 40 mM | 0.0408 mL | 0.2040 mL | 0.4080 mL | 1.0201 mL | |
| 50 mM | 0.0326 mL | 0.1632 mL | 0.3264 mL | 0.8161 mL | |
| 60 mM | 0.0272 mL | 0.1360 mL | 0.2720 mL | 0.6801 mL | |
| 80 mM | 0.0204 mL | 0.1020 mL | 0.2040 mL | 0.5101 mL | |
| 100 mM | 0.0163 mL | 0.0816 mL | 0.1632 mL | 0.4080 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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