H-D-Phe-Pip-Arg-pNA dihydrochloride
Based on 1 publication(s) in Google Scholar
H-D-Phe-Pip-Arg-pNA dihydrochloride (S-2238 dihydrochloride) is a specific chromogenic peptide substrate for thrombin (Thrombin). The amide bond of H-D-Phe-Pip-Arg-pNA dihydrochloride is rapidly hydrolyzed by thrombin, producing a characteristic yellow absorbance at 405 nm, and the change in absorbance is proportional to thrombin activity. H-D-Phe-Pip-Arg-pNA dihydrochloride can be used in biochemical detection-related studies.
For research use only. We do not sell to patients.
- CAS No.: 62354-65-8
- Formula: C27H38Cl2N8O5
- Molecular Weight:625.55
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications Citing Use of MedChemExpress (MCE) H-D-Phe-Pip-Arg-pNA dihydrochloride
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Biological Activity
Description
In Vitro
H-D-Phe-Pip-Arg-pNA (S-2238) (compound 3) dihydrochloride enables a sensitive, accurate thrombin-based chromogenic assay for heparin in human plasma, with a minimum detectable heparin concentration of 0.005 U/mL in normal plasma; the performance of this assay at low heparin concentrations is improved when purified human antithrombin III is used[1].
H-D-Phe-Pip-Arg-pNA dihydrochloride is the most reactive substrate for plasmin F-III-2 and F-III-1 purified from Lumbricus rubellus[2].
H-D-Phe-Pip-Arg-pNA dihydrochloride can be acted upon by plasmin purified from Lumbricus rubellus; these plasmins retain full amidolytic activity towards this substrate after incubation at 37°C and pH 1-11 for 30 min; the amidolytic activity of plasmin towards this substrate is strongly inhibited by DFP, SBTI and aprotinin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 62354-65-8
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Molecular Weight 625.55
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Formula C27H38Cl2N8O5
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Synonyms
S-2238 dihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Small
2026 Mar;22(13):e2500193. PMID: 40388659
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)