H-D-Phe-Pip-Arg-pNA
Based on 1 publication(s) in Google Scholar
H-D-Phe-Pip-Arg-pNA (S-2238) is a specific chromogenic peptide substrate for thrombin (Thrombin). The amide bond of H-D-Phe-Pip-Arg-pNA is rapidly hydrolyzed by thrombin, producing a characteristic yellow absorbance at 405 nm, and the change in absorbance is proportional to thrombin activity. H-D-Phe-Pip-Arg-pNA can be used in biochemical detection-related studies.
For research use only. We do not sell to patients.
- Purity: 95.98%
- CAS No.: 64815-81-2
- Formula: C27H36N8O5
- Molecular Weight:552.63
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) H-D-Phe-Pip-Arg-pNA
More
Biological Activity
H-D-Phe-Pip-Arg-pNA (S-2238) (compound 3) enables a sensitive, accurate thrombin-based chromogenic assay for heparin in human plasma, with a minimum detectable heparin concentration of 0.005 U/mL in normal plasma; the performance of this assay at low heparin concentrations is improved when purified human antithrombin III is used[1].
H-D-Phe-Pip-Arg-pNA is the most reactive substrate for plasmin F-III-2 and F-III-1 purified from Lumbricus rubellus[2].
H-D-Phe-Pip-Arg-pNA can be acted upon by plasmin purified from Lumbricus rubellus; these plasmins retain full amidolytic activity towards this substrate after incubation at 37°C and pH 1-11 for 30 min; the amidolytic activity of plasmin towards this substrate is strongly inhibited by DFP, SBTI and aprotinin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 64815-81-2
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Appearance Solid
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Molecular Weight 552.63
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Formula C27H36N8O5
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Color White to off-white
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Synonyms
S-2238
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Sequence Shortening
{D-Phe}-{Pip}-R-pNA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Small
2026 Mar;22(13):e2500193. PMID: 40388659
Solvent & Solubility
DMSO : 25 mg/mL (45.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8095 mL | 9.0476 mL | 18.0953 mL | 45.2382 mL |
| 5 mM | 0.3619 mL | 1.8095 mL | 3.6191 mL | 9.0476 mL | |
| 10 mM | 0.1810 mL | 0.9048 mL | 1.8095 mL | 4.5238 mL | |
| 15 mM | 0.1206 mL | 0.6032 mL | 1.2064 mL | 3.0159 mL | |
| 20 mM | 0.0905 mL | 0.4524 mL | 0.9048 mL | 2.2619 mL | |
| 25 mM | 0.0724 mL | 0.3619 mL | 0.7238 mL | 1.8095 mL | |
| 30 mM | 0.0603 mL | 0.3016 mL | 0.6032 mL | 1.5079 mL | |
| 40 mM | 0.0452 mL | 0.2262 mL | 0.4524 mL | 1.1310 mL |
H-D-Phe-Pip-Arg-pNA Related Classifications
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