H-D-Phe-Pip-Arg-pNA hydrochloride
Based on 1 publication(s) in Google Scholar
H-D-Phe-Pip-Arg-pNA hydrochloride (S-2238 hydrochloride) is a specific chromogenic peptide substrate for thrombin (Thrombin). The amide bond of H-D-Phe-Pip-Arg-pNA hydrochloride is rapidly hydrolyzed by thrombin, producing a characteristic yellow absorbance at 405 nm, and the change in absorbance is proportional to thrombin activity. H-D-Phe-Pip-Arg-pNA hydrochloride can be used in biochemical detection-related studies.
For research use only. We do not sell to patients.
- Purity: 98.44%
- CAS No.: 160192-34-7
- Formula: C27H37ClN8O5
- Molecular Weight:589.09
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) H-D-Phe-Pip-Arg-pNA hydrochloride
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Biological Activity
H-D-Phe-Pip-Arg-pNA (S-2238) (compound 3) hydrochloride enables a sensitive, accurate thrombin-based chromogenic assay for heparin in human plasma, with a minimum detectable heparin concentration of 0.005 U/mL in normal plasma; the performance of this assay at low heparin concentrations is improved when purified human antithrombin III is used[1].
H-D-Phe-Pip-Arg-pNA hydrochloride is the most reactive substrate for plasmin F-III-2 and F-III-1 purified from Lumbricus rubellus[2].
H-D-Phe-Pip-Arg-pNA hydrochloride can be acted upon by plasmin purified from Lumbricus rubellus; these plasmins retain full amidolytic activity towards this substrate after incubation at 37°C and pH 1-11 for 30 min; the amidolytic activity of plasmin towards this substrate is strongly inhibited by DFP, SBTI and aprotinin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 160192-34-7
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Appearance Solid
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Molecular Weight 589.09
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Formula C27H37ClN8O5
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Color Light yellow to yellow
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Synonyms
S-2238 hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Small
2026 Mar;22(13):e2500193. PMID: 40388659
Solvent & Solubility
H2O : 250 mg/mL (424.38 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.6975 mL | 8.4877 mL | 16.9753 mL | 42.4383 mL |
| 5 mM | 0.3395 mL | 1.6975 mL | 3.3951 mL | 8.4877 mL | |
| 10 mM | 0.1698 mL | 0.8488 mL | 1.6975 mL | 4.2438 mL | |
| 15 mM | 0.1132 mL | 0.5658 mL | 1.1317 mL | 2.8292 mL | |
| 20 mM | 0.0849 mL | 0.4244 mL | 0.8488 mL | 2.1219 mL | |
| 25 mM | 0.0679 mL | 0.3395 mL | 0.6790 mL | 1.6975 mL | |
| 30 mM | 0.0566 mL | 0.2829 mL | 0.5658 mL | 1.4146 mL | |
| 40 mM | 0.0424 mL | 0.2122 mL | 0.4244 mL | 1.0610 mL | |
| 50 mM | 0.0340 mL | 0.1698 mL | 0.3395 mL | 0.8488 mL | |
| 60 mM | 0.0283 mL | 0.1415 mL | 0.2829 mL | 0.7073 mL | |
| 80 mM | 0.0212 mL | 0.1061 mL | 0.2122 mL | 0.5305 mL | |
| 100 mM | 0.0170 mL | 0.0849 mL | 0.1698 mL | 0.4244 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
H-D-Phe-Pip-Arg-pNA hydrochloride Related Classifications
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