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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W181026
    KLF10-IN-1
    		99.84%
    KLF10-IN-1 (#48-15) is a KLF10 inhibitor with an IC50 value of 40 μM for KLF10 reporter gene. KLF10-IN-1 can inhibit KLF10-DNA binding and transcriptional activity, block the conversion of CD4+CD25 T cells to CD4+CD25+T regulatory cells, and inhibit the expression of KLF10 target genes. KLF10-IN-1 can be used as a useful mechanistic probe to study KLF10-mediated effects and T regulatory cell biology.
    KLF10-IN-1
  • HY-D1154
    β-Ala-Lys(AMCA)
    		99.25%
    β-Ala-Lys (AMCA) is a fluorescently labeled substrate of oligopeptide transporters. β-Ala-Lys (AMCA) acts as a substrate for a variety of bacterial proton-coupled oligopeptide transporters, and is used to label the activity of oligopeptide transporters. Excitation/emission wavelength: 340 nM/460 nM.
    β-Ala-Lys(AMCA)
  • HY-D2100B
    Cy5-DSPE chloride
    		99.13%
    Cy5-DSPE chloride is a far-infrared fluorescent phospholipid (λex/λem = 600/665 nm). Cy5-DSPE chloride is used to label liposomes, membranes and lipid nanoparticles, and can be applied to tracking and imaging studies of liposomes.
    Cy5-DSPE chloride
  • HY-D1045
    Dabcyl acid
    		98.61%
    Dabcyl acid (Dabcyl) is a nonfluorescent chromophore and a quencher. Dabcyl acid can be used as molecular beacon nucleic acid probes to recognize and report the presence of specific nucleic acids in homogeneous solutions.
    Dabcyl acid
  • HY-115691
    STY-BODIPY
    STY-BODIPY (Styrene-BODIPY; Styrene-Conjugated BODIPY) is a styrene-conjugated fluorescent probe used to measure the activity of radical-trapping antioxidants (RTAs)>.
    STY-BODIPY
  • HY-D0074
    Prodan
    		99.71%
    Prodan, a solvatochromic fluorophore, has been used as a microenvironment-sensitive membrane reporter. Based on the chemistry of Prodan, fluorescent nucleosides are designed and synthesized.The fluorescent nucleosides sensitively varied the Stokes shift values depending on the orientational polarizability of the solvent.
    Prodan
  • HY-NP164
    Concanavalin A-HRP
    Concanavalin A-HRP (Con A-HRP) is a HRP-labled Concanavalin A (HY-P2149). Concanavalin A is a Ca2+/Mn2+-dependent and mannose/glucose-binding plant lectin. Concanavalin A binds the N- and O-glycosylated proteins of head and neck carcinoma cells glycoproteins.
    Concanavalin A-HRP
  • HY-D0827
    Cy2
    		98.0%
    Cyanines are formally compounds with two nitrogen atoms linked by an odd number of methene units. 26 28 The nitrogen atoms are parts of the heterocyclic units (such as indole, benzoxazol, or benzothiazol) . The structures and optical properties of representative cyanine dyes used for in vivo imaging are presented. Cyanines are characterized by long wavelength, tunable absorption and emission, very high extinction coefficient (up to 300,000 M 1 cm 1), good water solubility, and relatively straightforward synthesis.
    Cy2
  • HY-110128
    Sulfidefluor 7-AM
    		99.9%
    Sulfidefluor 7-AM is a stable hydrogen sulphide (H2S) fluorescent probe. Sulfidefluor 7-AM is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Sulfidefluor 7-AM
  • HY-D0211
    Phenolphthalein
    		99.39%
    Phenolphthalein is an acid-base indicator that is colorless in an acidic environment and pink in an alkaline environment. Phenolphthalein is genotoxic and potentially carcinogenic to mice. In addition, phenolphthalein is also a blood indicator.
    Phenolphthalein
  • HY-117095
    4-Methylumbelliferyl oleate
    		99.58%
    4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).
    4-Methylumbelliferyl oleate
  • HY-113870
    6-NBDG
    		98.75%
    6-NBDG is a fluorescent glucose analogue, it can be used for fluorescence imaging and monitoring glucose transport and uptake. 6-NBDG can be used as a fluorescent probe for detecting macrophage-rich atherosclerotic plaques.
    6-NBDG
  • HY-P2377
    Ac-YVAD-AFC
    		99.90%
    Ac-YVAD-AFC is the the fluorometric peptide substrate of caspase-1, with λ excitation of 400 nm and λ emission of 505 nm.
    Ac-YVAD-AFC
  • HY-D0918
    YO-PRO-1
    		98.20%
    YO-PRO-1 is a non-cell-membrane-penetrating green fluorescent dye. YO-PRO-1 cannot penetrate normal cell membranes but can penetrate the membranes of apoptotic and necrotic cells. YO-PRO-1 has a high affinity for DNA. When it is not bound to DNA, it emits almost no fluorescence, and when it binds to DNA, it can emit green fluorescence (Ex/Em: 488/530±30 nM). YO-PRO-1 is often used together with Propidium Iodide (HY-D0815) to analyze and identify apoptotic and necrotic cells.
    YO-PRO-1
  • HY-P0286F1A
    FITC-OVA (323-339) TFA
    		98.22%
    FITC-OVA (323-339) TFA is a biological active peptide. (FITC labeled HY-P0286)
    FITC-OVA (323-339) TFA
  • HY-Y0695
    Amido Black 10B
    		99.76%
    Amido Black 10B (Naphthol Blue Black) is a highly toxic azo dye. Amido Black 10B is genotoxic and mutagenic. Amido Black 10B can cause respiratory problems. Amido Black 10B is used for amino acid dyeing.
    Amido Black 10B
  • HY-D1261
    Naph-EA-mal
    Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.
    Naph-EA-mal
  • HY-NP0147
    Wheat Germ Agglutinin (Fluorescein)
    Wheat Germ Agglutinin (WGA) Fluorescein is a classic fluorescent label that specifically binds to sugar residues such as N-acetylglucosamine, N-acetylneuraminic acid and sialic acid. Wheat Germ Agglutinin Fluorescein performs regionally differential fluorescent staining of the ocular surface epithelial glycocalyx to assess its integrity, and causes no damage to the eye at safe concentrations. Wheat Germ Agglutinin Fluorescein is also used for staining structures including red blood cells, cultured cells, bacteria and pine wood nematodes, and facilitates the isolation of wheat-associated plant-growth-promoting rhizobacterial strains. Wheat Germ Agglutinin Fluorescein can be applied to the detection of ocular glycocalyx integrity and the research of related diseases such as pine wilt disease.
    Wheat Germ Agglutinin (Fluorescein)
  • HY-D1735
    BODIPY TR Ceramide
    		99.26%
    The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY TR Ceramide (Golgi-Red Tracke) is a Golgi-specific fluorescent dye, which can visualise individual cells. Ex/Em=589 nm/616 nm.
    BODIPY TR Ceramide
  • HY-131027
    Janelia Fluor® 646, Azide
    		99.09%
    Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Janelia Fluor® 646, Azide
Cat. No. Product Name / Synonyms Application Reactivity