Ac-YVAD-AFC
Based on 4 publication(s) in Google Scholar
Ac-YVAD-AFC is a specifically designed fluorescent Caspase-1 substrate. Ac-YVAD-AFC can be specifically cleaved by activated Caspase-1, thereby releasing free AFC fluorophore for detection. Ac-YVAD-AFC is a powerful tool for quantitative analysis of enzyme activity, for example, playing a key role in studying TNF-α-induced Caspase-1 activation in adipocyte lysates. Ac-YVAD-AFC is widely used in mechanistic studies of inflammasome signaling pathways and related diseases such as sepsis.
For research use only. We do not sell to patients.
- Purity : 99.90%
- CAS No.: 219137-85-6
- Formula: C33H36F3N5O10
- Molecular Weight:719.66
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Ac-YVAD-AFC
MoreAll Caspase Isoforms
More
Biological Activity
Description
IC50 & Target
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Caspase-1 |
In Vitro
Ac-YVAD-AFC (50 μM; 2 h) effectively measures caspase-1 activity in differentiated mouse 3T3-L1 adipocytes, detecting a significant increase in activity after 6 h treatment with 20 ng/mL TNF-α that remains elevated despite pre-treatment with 2 μM PD0325901 (HY-10254)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
460
Chemical Information
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CAS No. 219137-85-6
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Appearance Solid
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Molecular Weight 719.66
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Formula C33H36F3N5O10
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Color White to off-white
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Sequence Shortening
Ac-YVAD-{AFC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (4)
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Journal Impact Factor
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Most Recent
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Food Chem
Transient autophagy inhibition strengthened postharvest tomato (Solanum lycopersicum) resistance against Botrytis cinerea through curtailing ROS-induced programmed cell death. [Abstract]2024 Oct 1:454:139811. PMID: 38820631 -
Int J Mol Sci
HERV-W Env Induces Neuron Pyroptosis via the NLRP3-CASP1-GSDMD Pathway in Recent-Onset Schizophrenia. [Abstract]2025 Jan 9;26(2):520. PMID: 39859234 -
Fish Shellfish Immunol
Paralichthys olivaceus GSDME-mediated pyroptosis is regulated by multiple caspases in different manners. [Abstract]2024 Dec:155:110002. PMID: 39491658 -
Genes (Basel)
Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus. [Abstract]2024 Apr 25;15(5):540. PMID: 38790170
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (138.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3895 mL | 6.9477 mL | 13.8955 mL | 34.7386 mL |
| 5 mM | 0.2779 mL | 1.3895 mL | 2.7791 mL | 6.9477 mL | |
| 10 mM | 0.1390 mL | 0.6948 mL | 1.3895 mL | 3.4739 mL | |
| 15 mM | 0.0926 mL | 0.4632 mL | 0.9264 mL | 2.3159 mL | |
| 20 mM | 0.0695 mL | 0.3474 mL | 0.6948 mL | 1.7369 mL | |
| 25 mM | 0.0556 mL | 0.2779 mL | 0.5558 mL | 1.3895 mL | |
| 30 mM | 0.0463 mL | 0.2316 mL | 0.4632 mL | 1.1580 mL | |
| 40 mM | 0.0347 mL | 0.1737 mL | 0.3474 mL | 0.8685 mL | |
| 50 mM | 0.0278 mL | 0.1390 mL | 0.2779 mL | 0.6948 mL | |
| 60 mM | 0.0232 mL | 0.1158 mL | 0.2316 mL | 0.5790 mL | |
| 80 mM | 0.0174 mL | 0.0868 mL | 0.1737 mL | 0.4342 mL | |
| 100 mM | 0.0139 mL | 0.0695 mL | 0.1390 mL | 0.3474 mL |
Ac-YVAD-AFC Related Classifications
HY-P2377 Related Classifications
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