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Naph-EA-mal (Synonyms: Thiol-green 1)

Cat. No.: HY-D1261
Handling Instructions

Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.

For research use only. We do not sell to patients.

Naph-EA-mal Chemical Structure

Naph-EA-mal Chemical Structure

CAS No. : 210292-65-2

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Description

Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells[1]. Ex: 488 nM; Em: 540 nM.

In Vitro

Imaging thiols in living cells[1]
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 µM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 µM), a weaker fluorescence is observed in cells[1].
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 µM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed[1].
The Hep G2 cells, oxidized by diamide (200 µM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 µM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 µM). The green fluorescent signal is detected again[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

391.42

Formula

C₂₂H₂₁N₃O₄

CAS No.

210292-65-2

SMILES

O=C1N(CCCC)C(C2=CC=C(NCCN3C(C=CC3=O)=O)C4=CC=CC1=C24)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

Naph-EA-malThiol-green 1OthersNEMthiol-disulfidethiolintracellularInhibitorinhibitorinhibit

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