Phenolphthalein

Name: Phenolphthalein
Brand: MedChemExpress
SKU: HY-D0211
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0211 Purity: 99.39%: Data Sheet
COA

SDS
Handling Instructions
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Phenolphthalein is an acid-base indicator that is colorless in an acidic environment and pink in an alkaline environment. Phenolphthalein is genotoxic and potentially carcinogenic to mice. In addition, phenolphthalein is also a blood indicator.

For research use only. We do not sell to patients.

Phenolphthalein Chemical Structure

CAS No. : 77-09-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
250 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Phenolphthalein:

Phenolphthalein (Standard) Get quote

1 Publications Citing Use of MCE Phenolphthalein

•Phytomedicine. 2025 Oct 16:148:157417. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
MT4	CC₅₀	58 μM Compound: 1a	In vitro cytotoxicity against mock infected MT-4 cells. In vitro cytotoxicity against mock infected MT-4 cells.	[PMID: 10377217]

In Vitro

Phenolphthalein (2.5-10 μg/ml, 24 h; 1.25-15 μg/ml, 8 d) induces an increase in transformed cells in the Syrian hamster embryo (SHE) cell transformation assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Phenolphthalein (800 and 2400 mg/kg; i.g.; once daily for 26 weeks, 2400 mg/kg; p.o.; daily in the diet for 26 weeks) is genotoxic to p53+/- mice and causes proliferation of polychromatic erythrocytes with micronuclei^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

318.33

Formula

C₂₀H₁₄O₄

CAS No.

77-09-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC(C2=CC=C(O)C=C2)(C3=CC=C(O)C=C3)C4=C1C=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (157.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1414 mL	15.7071 mL	31.4141 mL
5 mM	0.6283 mL	3.1414 mL	6.2828 mL
10 mM	0.3141 mL	1.5707 mL	3.1414 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.39%

Select Batch:

Data Sheet (281 KB) SDS (604 KB)

COA (260 KB) HNMR (274 KB) LCMS (101 KB)

Handling Instructions (2659 KB)

References

[1]. Fleischmann C, et al. Color indicator for supramolecular polymer chemistry: phenolphthalein-containing thermo- and pH-sensitive N-(Isopropyl)acrylamide copolymers and β-cyclodextrin complexation. Macromol Rapid Commun. 2013 Jul 12;34(13):1085-9. [Content Brief]

[2]. Higaki R S, et al. A study of the sensitivity, stability and specificity of phenolphthalein as an indicator test for blood[J]. Canadian Society of Forensic Science Journal, 1976, 9(3): 97-102.

[3]. Stoll R E, et al. Phenolphthalein and bisacodyl: assessment of genotoxic and carcinogenic responses in heterozygous p53 (+/−) mice and Syrian hamster embryo (SHE) assay[J]. Toxicological Sciences, 2006, 90(2): 440-450. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1414 mL	15.7071 mL	31.4141 mL	78.5353 mL
	5 mM	0.6283 mL	3.1414 mL	6.2828 mL	15.7071 mL
	10 mM	0.3141 mL	1.5707 mL	3.1414 mL	7.8535 mL
	15 mM	0.2094 mL	1.0471 mL	2.0943 mL	5.2357 mL
	20 mM	0.1571 mL	0.7854 mL	1.5707 mL	3.9268 mL
	25 mM	0.1257 mL	0.6283 mL	1.2566 mL	3.1414 mL
	30 mM	0.1047 mL	0.5236 mL	1.0471 mL	2.6178 mL
	40 mM	0.0785 mL	0.3927 mL	0.7854 mL	1.9634 mL
	50 mM	0.0628 mL	0.3141 mL	0.6283 mL	1.5707 mL
	60 mM	0.0524 mL	0.2618 mL	0.5236 mL	1.3089 mL
	80 mM	0.0393 mL	0.1963 mL	0.3927 mL	0.9817 mL
	100 mM	0.0314 mL	0.1571 mL	0.3141 mL	0.7854 mL