Dabcyl acid  (Synonyms: DABCYL; Para-methyl red)

Dabcyl acid (Dabcyl) is a nonfluorescent chromophore and a quencher. Dabcyl acid can be used as molecular beacon nucleic acid probes to recognize and report the presence of specific nucleic acids in homogeneous solutions^[1][2].

Dabcyl acid is paired with fluorophores (such as EDANS) for fluorescence resonance energy transfer (FRET). When the fluorophore (such as EDANS) and Dabcyl acid are close to each other, the fluorescence of the fluorophore will be quenched by Dabcyl acid. When the molecular beacon binds to the target nucleic acid, the fluorophore and the quencher are separated, and the fluorophore emits fluorescence, thereby realizing the detection of the target nucleic acid^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

269.30

C₁₅H₁₅N₃O₂

6268-49-1

Solid

Reddish brown to black

O=C(O)C1=CC=C(/N=N/C2=CC=C(N(C)C)C=C2)C=C1

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Tyagi S, et al. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol. 1996 Mar;14(3):303-8.  [Content Brief]

  [2]. Conlon P, et al. Pyrene excimer signaling molecular beacons for probing nucleic acids. J Am Chem Soc. 2008 Jan 9;130(1):336-42.  [Content Brief]