Wheat Germ Agglutinin (Fluorescein)
Based on 1 publication(s) in Google Scholar
Wheat Germ Agglutinin (WGA) Fluorescein is a classic fluorescent label that specifically binds to sugar residues such as N-acetylglucosamine, N-acetylneuraminic acid and sialic acid. Wheat Germ Agglutinin Fluorescein performs regionally differential fluorescent staining of the ocular surface epithelial glycocalyx to assess its integrity, and causes no damage to the eye at safe concentrations. Wheat Germ Agglutinin Fluorescein is also used for staining structures including red blood cells, cultured cells, bacteria and pine wood nematodes, and facilitates the isolation of wheat-associated plant-growth-promoting rhizobacterial strains. Wheat Germ Agglutinin Fluorescein can be applied to the detection of ocular glycocalyx integrity and the research of related diseases such as pine wilt disease.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Wheat Germ Agglutinin (Fluorescein)
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Biological Activity
Wheat Germ Agglutinin (Fluorescein) (0.001-10%; measured immediately in cuvette) shows a linear fluorescent intensity response in a cell-free cuvette system, with no interference from 10% fetal bovine serum[1].
Wheat Germ Agglutinin (Fluorescein) (2 mg/mL; 30 min) stains 32 rhizobacterial isolates from wheat rhizosphere[3].
Wheat Germ Agglutinin Fluorescein (0.01% [w/v]; 1 h) stains only the vulvas of female and spicule holes of male Bursaphelenchus xylophilus (isolate S-10) grown on Botrytis cinerea mycelium, with minimal cuticle staining and no egg staining[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rabbit[1]
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Dosage:10%
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Administration:ocular instillation; 5 times at 10-minute intervals
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Result:Detected no signs of inflammation or damage immediately and 24 hours after instillation.
Chemical Information
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Appearance Solid
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Color Light yellow to orange
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SMILES
[Wheat Germ Agglutinin (Fluorescein)]
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Synonyms
WGA (Fluorescein)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
Purity & Documentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Mochizuki H, et al. Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein. Clin Ophthalmol. 2010;4:925-930. Published 2010 Aug 19. [Content Brief]
[3]. Zhang J, et al. Isolation and characterization of plant growth-promoting rhizobacteria from wheat roots by wheat germ agglutinin labeled with fluorescein isothiocyanate. J Microbiol. 2012;50(2):191-198. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Wheat Germ Agglutinin (Fluorescein) Related Classifications
HY-NP0147 Related Classifications
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- Wheat Germ Agglutinin (Fluorescein)
- WGA (Fluorescein)
- Fluorescent Dye
- plant growth-promoting rhizobacteria
- N-acetyl glucosamine
- N-acetylglucosaminyl sugar residues
- Pinus thunbergii
- N-acetyl neuraminic acid
- Bursaphelenchus xylophilus
- ocular surface epithelium glycocalyx
- red blood cells
- pine wood nematode
- sialic acid
- Inhibitor
- inhibitor
- inhibit