Wheat Germ Agglutinin (Fluorescein)  (Synonyms: WGA (Fluorescein))

Wheat Germ Agglutinin (WGA) Fluorescein is a classic fluorescent label that specifically binds to sugar residues such as N-acetylglucosamine, N-acetylneuraminic acid and sialic acid. Wheat Germ Agglutinin Fluorescein performs regionally differential fluorescent staining of the ocular surface epithelial glycocalyx to assess its integrity, and causes no damage to the eye at safe concentrations. Wheat Germ Agglutinin Fluorescein is also used for staining structures including red blood cells, cultured cells, bacteria and pine wood nematodes, and facilitates the isolation of wheat-associated plant-growth-promoting rhizobacterial strains. Wheat Germ Agglutinin Fluorescein can be applied to the detection of ocular glycocalyx integrity and the research of related diseases such as pine wilt disease^[1][2][3][4].

Wheat Germ Agglutinin (Fluorescein) (0.001-10%; measured immediately in cuvette) shows a linear fluorescent intensity response in a cell-free cuvette system, with no interference from 10% fetal bovine serum^[1].
Wheat Germ Agglutinin (Fluorescein) (2 mg/mL; 30 min) stains 32 rhizobacterial isolates from wheat rhizosphere^[3].
Wheat Germ Agglutinin Fluorescein (0.01% [w/v]; 1 h) stains only the vulvas of female and spicule holes of male Bursaphelenchus xylophilus (isolate S-10) grown on Botrytis cinerea mycelium, with minimal cuticle staining and no egg staining^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Wheat Germ Agglutinin Fluorescein (10%; ocular instillation; 5 times at 10-minute intervals) shows no apparent adverse effects on the rabbit ocular surface^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

Light yellow to orange

[Wheat Germ Agglutinin (Fluorescein)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mochizuki H, et al. Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein. Clin Ophthalmol. 2010;4:925-930. Published 2010 Aug 19.  [Content Brief]

  [2]. Model M A, et al. Measurement of wheat germ agglutinin binding with a fluorescence microscope[J]. Cytometry Part A: The Journal of the International Society for Advancement of Cytometry, 2009, 75(10): 874-881.

  [3]. Zhang J, et al. Isolation and characterization of plant growth-promoting rhizobacteria from wheat roots by wheat germ agglutinin labeled with fluorescein isothiocyanate. J Microbiol. 2012;50(2):191-198.  [Content Brief]

  [4]. Komatsu M, et al. Fluorescein-labeled wheat germ agglutinin stains the pine wood nematode, Bursaphelenchus xylophilus[J]. Journal of Forest Research, 2008, 13(2): 132-136.