Cy5-DSPE chloride
Based on 1 Customer Validation
Cy5-DSPE chloride is a far-infrared fluorescent phospholipid (λex/λem = 600/665 nm). Cy5-DSPE chloride is used to label liposomes, membranes and lipid nanoparticles, and can be applied to tracking and imaging studies of liposomes.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.13%
- 分子式: C73H119ClN3O9P
- 分子量:1249.17
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保管条件:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
生物活性
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.\tStorage: Store powder at -20°C, protected from light.
2.\tDissolution: Dissolve Cy5-DSPE in chloroform, DMSO (HY-Y0320C), or ethanol to create a stock solution.
3.\tThin-Film Method:
3.1 Mix Cy5-DSPE stock solution with other lipids (e.g., PC, Cholesterol) in a round-bottom flask.
3.2 Evaporate the solvent (e.g., using a rotary evaporator) to form a thin lipid film.
3.3 Dry the film under vacuum to remove residual solvent.
3.4 Hydrate the film with buffer (e.g., PBS) and sonicate or extrude to form liposomes.
4.\tInsertion Method: Alternatively, add small amounts of Cy5-DSPE solution directly into pre-formed vesicle suspensions and incubate at a temperature near the lipid transition temperature to allow insertion into the membrane.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Solid
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分子量 1249.17
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分子式 C73H119ClN3O9P
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Color Brown to black
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SMILES
CCCCCCCCCCCCCCCCCC(O[C@H](COC(CCCCCCCCCCCCCCCCC)=O)COP(OCCNC(CCCCCN1C2=CC=CC=C2C(C)(/C1=C\C=C\C=C\C3=[N+](C4=CC=CC=C4C3(C)C)C)C)=O)(O)=O)=O.[Cl-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
Ethanol : 10 mg/mL (8.01 mM; ultrasonic and warming and heat to 60°C)
DMSO : 4.17 mg/mL (3.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (268 KB)
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SDS (251 KB)
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- Deutsch - DE (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 0.8005 mL | 4.0027 mL | 8.0053 mL | 20.0133 mL |
| Ethanol | 5 mM | 0.1601 mL | 0.8005 mL | 1.6011 mL | 4.0027 mL |