Cy5-DSPE chloride
Based on 1 Customer Validation
Cy5-DSPE chloride is a far-infrared fluorescent phospholipid (λex/λem = 600/665 nm). Cy5-DSPE chloride is used to label liposomes, membranes and lipid nanoparticles, and can be applied to tracking and imaging studies of liposomes.
For research use only. We do not sell to patients.
- Purity: 99.13%
- Formula: C73H119ClN3O9P
- Molecular Weight:1249.17
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.\tStorage: Store powder at -20°C, protected from light.
2.\tDissolution: Dissolve Cy5-DSPE in chloroform, DMSO (HY-Y0320C), or ethanol to create a stock solution.
3.\tThin-Film Method:
3.1 Mix Cy5-DSPE stock solution with other lipids (e.g., PC, Cholesterol) in a round-bottom flask.
3.2 Evaporate the solvent (e.g., using a rotary evaporator) to form a thin lipid film.
3.3 Dry the film under vacuum to remove residual solvent.
3.4 Hydrate the film with buffer (e.g., PBS) and sonicate or extrude to form liposomes.
4.\tInsertion Method: Alternatively, add small amounts of Cy5-DSPE solution directly into pre-formed vesicle suspensions and incubate at a temperature near the lipid transition temperature to allow insertion into the membrane.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1249.17
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Formula C73H119ClN3O9P
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Color Brown to black
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SMILES
CCCCCCCCCCCCCCCCCC(O[C@H](COC(CCCCCCCCCCCCCCCCC)=O)COP(OCCNC(CCCCCN1C2=CC=CC=C2C(C)(/C1=C\C=C\C=C\C3=[N+](C4=CC=CC=C4C3(C)C)C)C)=O)(O)=O)=O.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
Ethanol : 10 mg/mL (8.01 mM; ultrasonic and warming and heat to 60°C)
DMSO : 4.17 mg/mL (3.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 0.8005 mL | 4.0027 mL | 8.0053 mL | 20.0133 mL |
| Ethanol | 5 mM | 0.1601 mL | 0.8005 mL | 1.6011 mL | 4.0027 mL |
Cy5-DSPE chloride Related Classifications
HY-D2100B Related Classifications
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