Cy5-DSPE chloride 

Cy5-DSPE chloride is a far-infrared fluorescent phospholipid (λex/λem = 600/665 nm). Cy5-DSPE chloride is used to label liposomes, membranes and lipid nanoparticles, and can be applied to tracking and imaging studies of liposomes^[1].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.\tStorage: Store powder at -20°C, protected from light.
2.\tDissolution: Dissolve Cy5-DSPE in chloroform, DMSO (HY-Y0320C), or ethanol to create a stock solution.
3.\tThin-Film Method:
3.1 Mix Cy5-DSPE stock solution with other lipids (e.g., PC, Cholesterol) in a round-bottom flask.
3.2 Evaporate the solvent (e.g., using a rotary evaporator) to form a thin lipid film.
3.3 Dry the film under vacuum to remove residual solvent.
3.4 Hydrate the film with buffer (e.g., PBS) and sonicate or extrude to form liposomes.
4.\tInsertion Method: Alternatively, add small amounts of Cy5-DSPE solution directly into pre-formed vesicle suspensions and incubate at a temperature near the lipid transition temperature to allow insertion into the membrane.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1249.17

C₇₃H₁₁₉ClN₃O₉P

Solid

Brown to black

CCCCCCCCCCCCCCCCCC(O[C@H](COC(CCCCCCCCCCCCCCCCC)=O)COP(OCCNC(CCCCCN1C2=CC=CC=C2C(C)(/C1=C\C=C\C=C\C3=[N+](C4=CC=CC=C4C3(C)C)C)C)=O)(O)=O)=O.[Cl-]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Cauzzo J, et al. Following the Fate of Dye-Containing Liposomes In Vitro. Int J Mol Sci. 2020;21(14):4847. Published 2020 Jul 9.  [Content Brief]