Cy5-DSPE chloride ammonium 

Cy5-DSPE chloride ammonium is a far-infrared fluorescent phospholipid (λex/λem = 600/665 nm). Cy5-DSPE chloride ammonium is used to label liposomes, membranes and lipid nanoparticles, and can be applied to tracking and imaging studies of liposomes^[1].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.\tStorage: Store powder at -20°C, protected from light.
2.\tDissolution: Dissolve Cy5-DSPE in chloroform, DMSO (HY-Y0320C), or ethanol to create a stock solution.
3.\tThin-Film Method:
3.1 Mix Cy5-DSPE stock solution with other lipids (e.g., PC, Cholesterol) in a round-bottom flask.
3.2 Evaporate the solvent (e.g., using a rotary evaporator) to form a thin lipid film.
3.3 Dry the film under vacuum to remove residual solvent.
3.4 Hydrate the film with buffer (e.g., PBS) and sonicate or extrude to form liposomes.
4.\tInsertion Method: Alternatively, add small amounts of Cy5-DSPE solution directly into pre-formed vesicle suspensions and incubate at a temperature near the lipid transition temperature to allow insertion into the membrane.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1266.20

C₇₃H₁₂₂ClN₄O₉P

2260669-69-8

CCCCCCCCCCCCCCCCCC(O[C@H](COC(CCCCCCCCCCCCCCCCC)=O)COP(OCCNC(CCCCCN1C2=CC=CC=C2C(C)(/C1=C\C=C\C=C\C3=[N+](C4=CC=CC=C4C3(C)C)C)C)=O)(O)=O)=O.[Cl-].N

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Cauzzo J, et al. Following the Fate of Dye-Containing Liposomes In Vitro. Int J Mol Sci. 2020;21(14):4847. Published 2020 Jul 9.  [Content Brief]