Janelia Fluor® 646, Azide
Based on 1 Customer Validation
Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 646/664 nm).
For research use only. We do not sell to patients.
- Purity: 99.09%
- Formula: C37H44N6O6Si
- Molecular Weight:696.87
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as azide serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
JF646 can serve as a ligand for self-labeling tag (such as HaloTag)[1].
Janelia Fluor 646, Azide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
646
605
Chemical Information
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Appearance Solid
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Molecular Weight 696.87
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Formula C37H44N6O6Si
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Color Light green to green
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SMILES
O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CCC4)=C(C3=C/5)C=CC5=[N+]6CCC/6)=C1)C([O-])=O)NCCOCCOCCOCCN=[N+]=[N-]
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Synonyms
JF646, Azide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (143.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403. [Content Brief]
[2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4350 mL | 7.1749 mL | 14.3499 mL | 35.8747 mL |
| 5 mM | 0.2870 mL | 1.4350 mL | 2.8700 mL | 7.1749 mL | |
| 10 mM | 0.1435 mL | 0.7175 mL | 1.4350 mL | 3.5875 mL | |
| 15 mM | 0.0957 mL | 0.4783 mL | 0.9567 mL | 2.3916 mL | |
| 20 mM | 0.0717 mL | 0.3587 mL | 0.7175 mL | 1.7937 mL | |
| 25 mM | 0.0574 mL | 0.2870 mL | 0.5740 mL | 1.4350 mL | |
| 30 mM | 0.0478 mL | 0.2392 mL | 0.4783 mL | 1.1958 mL | |
| 40 mM | 0.0359 mL | 0.1794 mL | 0.3587 mL | 0.8969 mL | |
| 50 mM | 0.0287 mL | 0.1435 mL | 0.2870 mL | 0.7175 mL | |
| 60 mM | 0.0239 mL | 0.1196 mL | 0.2392 mL | 0.5979 mL | |
| 80 mM | 0.0179 mL | 0.0897 mL | 0.1794 mL | 0.4484 mL | |
| 100 mM | 0.0143 mL | 0.0717 mL | 0.1435 mL | 0.3587 mL |
Janelia Fluor® 646, Azide Related Classifications
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