Janelia Fluor® 646, Maleimide
Based on 1 Customer Validation
Janelia Fluor 646, Maleimide (JF646, Maleimide) is a red fluorescent dye that contains a maleimide group. JF646, Maleimide can be used in cellular imaging. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute (Ex/Em = 646/664 nm).
For research use only. We do not sell to patients.
- Purity: 97.50%
- Formula: C35H34N4O5Si
- Molecular Weight:618.75
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as Maleimide serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
JF646 can serve as a ligand for self-labeling tag (such as HaloTag)[1].
JF646, Maleimide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
646
605
Chemical Information
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Appearance Solid
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Molecular Weight 618.75
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Formula C35H34N4O5Si
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Color Light green to green
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SMILES
O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CCC4)=C(C3=C/5)C=CC5=[N+]6CCC/6)=C1)C([O-])=O)NCCN7C(C=CC7=O)=O
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Synonyms
JF646, Maleimide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (80.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256 [Content Brief]
[2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6162 mL | 8.0808 mL | 16.1616 mL | 40.4040 mL |
| 5 mM | 0.3232 mL | 1.6162 mL | 3.2323 mL | 8.0808 mL | |
| 10 mM | 0.1616 mL | 0.8081 mL | 1.6162 mL | 4.0404 mL | |
| 15 mM | 0.1077 mL | 0.5387 mL | 1.0774 mL | 2.6936 mL | |
| 20 mM | 0.0808 mL | 0.4040 mL | 0.8081 mL | 2.0202 mL | |
| 25 mM | 0.0646 mL | 0.3232 mL | 0.6465 mL | 1.6162 mL | |
| 30 mM | 0.0539 mL | 0.2694 mL | 0.5387 mL | 1.3468 mL | |
| 40 mM | 0.0404 mL | 0.2020 mL | 0.4040 mL | 1.0101 mL | |
| 50 mM | 0.0323 mL | 0.1616 mL | 0.3232 mL | 0.8081 mL | |
| 60 mM | 0.0269 mL | 0.1347 mL | 0.2694 mL | 0.6734 mL | |
| 80 mM | 0.0202 mL | 0.1010 mL | 0.2020 mL | 0.5051 mL |
Janelia Fluor® 646, Maleimide Related Classifications
HY-131029 Related Classifications
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