2. Cell Cycle/DNA Damage Epigenetics
  3. Sirtuin
  4. Thiomyristoyl

Thiomyristoyl is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM.

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CAS 番号 : 1429749-41-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 155 在庫あり
Solution
10 mM * 1 mL in DMSO USD 155 在庫あり
Solid
1 mg $66 在庫あり
5 mg $121 在庫あり
10 mg $165 在庫あり
25 mg $319 在庫あり
50 mg $539 在庫あり
100 mg $825 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 5 publication(s) in Google Scholar

Other Forms of Thiomyristoyl:

Thiomyristoyl 関連抗体

Top Publications Citing Use of Products

    Thiomyristoyl purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 May;21(5):429-441.  [Abstract]

    HuCCT1 and RBE cells are treated with 0, 25, or 50 μm Tm (Thiomyristoyl) and subjected to western blotting

    Thiomyristoyl purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 May;21(5):429-441.  [Abstract]

    After treatment with Tm (Thiomyristoyl), NAC, or a combination of both, the levels of ROS and proteins are detected.

    Thiomyristoyl purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 May;21(5):429-441.  [Abstract]

    cMYC, p-PDHA1, PHGDH, PSAT1 and PSPH protein levels in xenograft tissues from control and Tm (Thiomyristoyl) group are detected by staining and Western blotting.

    Sirtuin アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7 SIRT4

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Thiomyristoyl is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM.

    IC50 & Target[1]

    SIRT2

    28 nM (IC50)

    SIRT1

    98 μM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    A549 GI50
    17.3 μM
    Compound: TM
    Growth inhibition of human A549 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human A549 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    BXPC-3 GI50
    13.3 μM
    Compound: TM
    Growth inhibition of human BxPC3 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human BxPC3 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    HCT-116 GI50
    131.6 μM
    Compound: TM
    Growth inhibition of human HCT116 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human HCT116 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    HCT-116 GI50
    16.7 μM
    Compound: TM
    Inhibition of anchorage-independent cell growth in human HCT116 cells assessed as reduction in colony formation incubated for 5 days followed by compound re-addition and measured after 9 to 11 days by nitro blue tetrazolium chloride-based soft agar colony
    Inhibition of anchorage-independent cell growth in human HCT116 cells assessed as reduction in colony formation incubated for 5 days followed by compound re-addition and measured after 9 to 11 days by nitro blue tetrazolium chloride-based soft agar colony
    		[PMID: 30986062]
    MCF7 GI50
    37 μM
    Compound: TM
    Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    MDA-MB-231 GI50
    42.8 μM
    Compound: TM
    Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    MDA-MB-468 GI50
    15.7 μM
    Compound: TM
    Growth inhibition of human MDA-MB-468 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human MDA-MB-468 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    NCI-H23 GI50
    16.4 μM
    Compound: TM
    Growth inhibition of human NCI-H23 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human NCI-H23 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    SW948 GI50
    19.2 μM
    Compound: TM
    Growth inhibition of human SW948 cells after 72 hrs by CellTiter-Blue assay
    Growth inhibition of human SW948 cells after 72 hrs by CellTiter-Blue assay
    		[PMID: 30986062]
    体外実験

    Thiomyristoyl (TM) is a potent SIRT2-specific inhibitor with broad anticancer activity but little effect on non-cancerous cells. SIRT2-inhibition promotes c-Myc ubiquitination and degradation, suggesting the therapeutic potential of TM to target certain c-Myc-driven cancers. TM could inhibit SIRT2 with an IC50 of 28 nM, but inhibits SIRT1 with an IC50 value of 98 μM and does not inhibit SIRT3 even at 200 μM. TM inhibits three human breast cancer cell lines, MCF-7, MDA-MB-468, and MDA-MB-231[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Thiomyristoyl 関連抗体
    体内実験

    TM inhibits tumor growth in mouse models of breast cancer. TM does not cause significant toxicity in mice and no significant weight loss is observed in TM-treated mice. S5H, the acetyl-a-tubulin level is moderately but statistically significantly increased in tumors from TM-treated mice compared with those from vehicle-treated mice, suggesting that TM indeed inhibits SIRT2 in vivo[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    581.85

    分子式

    C34H51N3O3S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC1=CC=CC=C1)N[C@H](C(NC2=CC=CC=C2)=O)CCCCNC(CCCCCCCCCCCCC)=S
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 32 mg/mL (55.00 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 15.29 mg/mL (26.28 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7187 mL 8.5933 mL 17.1866 mL
    5 mM 0.3437 mL 1.7187 mL 3.4373 mL
    10 mM 0.1719 mL 0.8593 mL 1.7187 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 98.23%

    COA (242 KB) HNMR (145 KB) LCMS (112 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [1]

    Cells are seeded into 96-well plates at 3,000–4,000 cells per well. After 24 hr, test compounds (Thiomyristoyl) are added to cells to final concentrations ranging from 1 to 50 μM. Cells are then incubated for 72 hr and cell viability is measured using the CellTiter-Blue viability assay. Relative cell viability in the presence of test compounds is normalized to the vehicle-treated controls after background subtraction. GraphPad Prism software is used to determine the IC50 values. Knockdown of SIRT1-7 in various cell lines is achieved by lentiviral infection[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 1.7187 mL 8.5933 mL 17.1866 mL 42.9664 mL
    5 mM 0.3437 mL 1.7187 mL 3.4373 mL 8.5933 mL
    10 mM 0.1719 mL 0.8593 mL 1.7187 mL 4.2966 mL
    15 mM 0.1146 mL 0.5729 mL 1.1458 mL 2.8644 mL
    20 mM 0.0859 mL 0.4297 mL 0.8593 mL 2.1483 mL
    25 mM 0.0687 mL 0.3437 mL 0.6875 mL 1.7187 mL
    DMSO 30 mM 0.0573 mL 0.2864 mL 0.5729 mL 1.4322 mL
    40 mM 0.0430 mL 0.2148 mL 0.4297 mL 1.0742 mL
    50 mM 0.0344 mL 0.1719 mL 0.3437 mL 0.8593 mL
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    Thiomyristoyl Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Thiomyristoyl1429749-41-6SirtuinInhibitorinhibitorinhibit

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    製品名:
    Thiomyristoyl
    製品番号:
    HY-101278
    数量:
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