2. Protein Tyrosine Kinase/RTK
  3. Ephrin Receptor
  4. ALW-II-49-7

ALW-II-49-7 is a selective EphB2 kinase inhibitor with an EC50 value of 40 nM in cell.

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ALW-II-49-7

ALW-II-49-7 構造式

CAS 番号 : 1135219-23-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 226 在庫あり
Solution
10 mM * 1 mL in DMSO USD 226 在庫あり
Solid
1 mg $82 在庫あり
5 mg $206 在庫あり
10 mg $310 在庫あり
25 mg $560 在庫あり
50 mg $850 在庫あり
100 mg $1280 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

ALW-II-49-7 関連抗体

Top Publications Citing Use of Products

Ephrin Receptor アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
EphB EphA

  • 生物活性

  • 純度とドキュメンテーション

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製品説明

ALW-II-49-7 is a selective EphB2 kinase inhibitor with an EC50 value of 40 nM in cell[1].

IC50 & Target

EphB2[1]
Cellular Effect
Cell Line Type Value Description References
HEK293 IC50
4.799 nM
Compound: 1
Inhibition of FLAG-tagged DDR1 in human HEK293 cells assessed as reduction in collagen-1-induced DDR1 phosphorylation incubated for 18 hrs by ELISA
Inhibition of FLAG-tagged DDR1 in human HEK293 cells assessed as reduction in collagen-1-induced DDR1 phosphorylation incubated for 18 hrs by ELISA
[PMID: 31938459]
U-87MG ATCC EC50
100 nM
Compound: 9
Inhibition of ephrin-B1-induced autophosphorylation of EphB2 expressed in human U87 cells after 1 hr by Western blot analysis
Inhibition of ephrin-B1-induced autophosphorylation of EphB2 expressed in human U87 cells after 1 hr by Western blot analysis
[PMID: 19553108]
体外実験

ALW-II-49-7 (compound 9) exhibits selectivity profile of binding to a set of kinases including b-raf, CSF1R, DDR1, DDR2, EphA2, EphA5, EphA8, EphB1, EphB2, EphB3, Frk, Kit, Lck, p38a, p38b, PDGFRa, PDGFRb, and Raf1[1].
ALW-II-49-7 (0.01-10 μM; 1 h) inhibits EphB2 tyrosine kinase activity in U87 glioblastoma cells. EphB2 is added in media at 2 μg/mL[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ALW-II-49-7 関連抗体
分子量

414.38

分子式

C21H17F3N4O2
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC(NC2=CC(C(NC3=CC=CC(C(F)(F)F)=C3)=O)=CC=C2C)=CN=C1)N
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (241.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4132 mL 12.0662 mL 24.1324 mL
5 mM 0.4826 mL 2.4132 mL 4.8265 mL
10 mM 0.2413 mL 1.2066 mL 2.4132 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.97%

COA (244 KB) HNMR (260 KB) LCMS (100 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4132 mL 12.0662 mL 24.1324 mL 60.3311 mL
5 mM 0.4826 mL 2.4132 mL 4.8265 mL 12.0662 mL
10 mM 0.2413 mL 1.2066 mL 2.4132 mL 6.0331 mL
15 mM 0.1609 mL 0.8044 mL 1.6088 mL 4.0221 mL
20 mM 0.1207 mL 0.6033 mL 1.2066 mL 3.0166 mL
25 mM 0.0965 mL 0.4826 mL 0.9653 mL 2.4132 mL
30 mM 0.0804 mL 0.4022 mL 0.8044 mL 2.0110 mL
40 mM 0.0603 mL 0.3017 mL 0.6033 mL 1.5083 mL
50 mM 0.0483 mL 0.2413 mL 0.4826 mL 1.2066 mL
60 mM 0.0402 mL 0.2011 mL 0.4022 mL 1.0055 mL
80 mM 0.0302 mL 0.1508 mL 0.3017 mL 0.7541 mL
100 mM 0.0241 mL 0.1207 mL 0.2413 mL 0.6033 mL
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ALW-II-49-7 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ALW-II-49-71135219-23-6Ephrin ReceptorInhibitorinhibitorinhibit

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製品名:
ALW-II-49-7
製品番号:
HY-18833
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