2. Apoptosis Metabolic Enzyme/Protease
  3. MDM-2/p53 Apoptosis Endogenous Metabolite
  4. C16-Ceramide (d18:1/16:0)

C16-Ceramide  (Synonyms: C16セラミド)

製品番号: HY-100354 純度: 99.92%
COA 取扱説明書 Technical Support

C16-Ceramide (d18:1/16:0) is a natural small molecule activating p53 through the direct and selective binding.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 24696-26-2

容量 価格（税別） 在庫状況 数量
Solution
10 mM * 1 mL in Ethanol USD 58 在庫あり
Solid
5 mg $49 在庫あり
10 mg $74 在庫あり
25 mg $146 在庫あり
50 mg $220 在庫あり
100 mg $360 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 7 publication(s) in Google Scholar

Other Forms of C16-Ceramide (d18:1/16:0):

C16-Ceramide 関連抗体

Top Publications Citing Use of Products

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Dec 1:19:10645-10660.  [Abstract]

    The relative mRNA levels of TFRC and ACSL4 in SH-SY5Y cells treated with C16-Ceramide (50 μM).

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Dec 1:19:10645-10660.  [Abstract]

    Western blot analysis of C16-Ceramide (50 μM) confirmed that activation of p53 weakened the inhibitory effect of EMD on ferroptosis.

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Lipids Health Dis. 2025 Apr 12;24(1):138.  [Abstract]

    Western blot analysis of the expression of P53, SHISA5, type I collagen, MMP2, p-IRE1α, GRP78, p-JNK, P62 and LC3-II proteins in HCF cells after activation of P53 by C16-ceramide (10 µM, 24 h).

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Jul 25;17(14):13358-13376.  [Abstract]

    C16-Ceramide (5 μM/10 μM). Cell viability assessed by live (green)/dead (red) staining.

    C16-Ceramide (d18:1/16:0) purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Dec 1:522:105-118.  [Abstract]

    Cell proliferation experiments were conducted in PANC-1 shNT, shCerS6+EV, shCerS6+rWT, and shCerS6+rR131A cell lines with or without different doses of C16-ceramide, and in CFPAC-1 EV, WT, or R131A cell lines with or without different doses of cell-permeable C16-ceramide (PC16: 0.5 μM/12.5 μM).

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    C16-Ceramide (d18:1/16:0) is a natural small molecule activating p53 through the direct and selective binding[1].

    IC50 & Target

    p53[1], Apoptosis[2]
    体外実験

    C16-Ceramide (d18:1/16:0) interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide (d18:1/16:0) in the cell[1].
    C16-Ceramide (d18:1/16:0) (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2].
    C16-Ceramide (d18:1/16:0) (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2].
    C16-Ceramide (d18:1/16:0) (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-Ceramide (d18:1/16:0) down-regulates Mdm2 expression via Btf[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    C16-Ceramide 関連抗体

    Cell Viability Assay[2]

    Cell Line: HCT116 cells
    Concentration: 2.5, 5, 10, 12, 20, 50 µM
    Incubation Time: 0-48 h
    Result: Strongly decreased cell viability in a time- and concentration-dependent manner.

    Western Blot Analysis[2]

    Cell Line: HCT116 cells
    Concentration: 12 μM
    Incubation Time: 1, 3 and 6 h
    Result: Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion also partially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.
    分子量

    537.90

    分子式

    C34H67NO3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCCCCCCCCCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMF : 20 mg/mL (37.18 mM; ultrasonic and warming and heat to 60°C)

    Ethanol : 10 mg/mL (18.59 mM; ultrasonic and warming and heat to 60°C)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8591 mL 9.2954 mL 18.5908 mL
    5 mM 0.3718 mL 1.8591 mL 3.7182 mL
    10 mM 0.1859 mL 0.9295 mL 1.8591 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    90% Corn Oil

      Solubility: ≥ 2 mg/mL (3.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% HPMC/1% Tween-80 in Saline water

      Solubility: 4.95 mg/mL (9.20 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  15% Solutol HS 15    10% Cremophor EL    35% PEG400    40% Water

      Solubility: 5 mg/mL (9.30 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.92%

    COA (238 KB) HNMR (264 KB) RP-HPLC (231 KB) MS (77 KB) Elemental Analysis Report (96 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMF 1 mM 1.8591 mL 9.2954 mL 18.5908 mL 46.4770 mL
    5 mM 0.3718 mL 1.8591 mL 3.7182 mL 9.2954 mL
    10 mM 0.1859 mL 0.9295 mL 1.8591 mL 4.6477 mL
    15 mM 0.1239 mL 0.6197 mL 1.2394 mL 3.0985 mL
    DMF 20 mM 0.0930 mL 0.4648 mL 0.9295 mL 2.3239 mL
    25 mM 0.0744 mL 0.3718 mL 0.7436 mL 1.8591 mL
    30 mM 0.0620 mL 0.3098 mL 0.6197 mL 1.5492 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    C16-Ceramide (d18:1/16:0) Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    C16-Ceramide (d18:1/16:0)24696-26-2C16-CeramideMDM-2/p53ApoptosisEndogenous MetaboliteHCT116BtfBcl-2-associated transcription factorBAXInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    C16-Ceramide (d18:1/16:0)
    製品番号:
    HY-100354
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.