ddATP  (Synonyms: 2',3'-Dideoxyadenosine 5'-triphosphate)

ddATP (2',3'-Dideoxyadenosine 5'-triphosphate), an active metabolite of 2',3'-dideoxyinosine, is a chain-elongating inhibitor of DNA polymerase. ddATP can be used for Sanger method for DNA sequencing and research of virus infection^[1][2][4].

ddATP (0.05-50 μM, 30 min) inhibits adenovirus DNA synthesis in isolated nuclei from adenovirus-infected KB cells^[1].
ddATP (100 μM, 5 h) reduces the number of γH2A.X foci in the DSB (DNA double-strand breaks) oocytes^[3].
ddATP inhibits HIV replication by inhibiting HIV reverse transcriptase^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

475.18

C₁₀H₁₆N₅O₁₁P₃

24027-80-3

Liquid

Colorless to light yellow

NC1=NC=NC2=C1N=CN2[C@H]3CC[C@H](O3)COP(O)(OP(OP(O)(O)=O)(O)=O)=O

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, 2 years

• [1]. van der Vliet PC, et al. Role of DNA polymerase gamma in adenovirus DNA replication. Mechanism of inhibition by 2',3'-dideoxynucleoside 5'-triphosphates. Biochemistry. 1981 Apr 28;20(9):2628-32.  [Content Brief]

  [2]. Xu Lan, et al. Simultaneous determination of 2 ,3 -dideoxyinosine and the active metabolite, 2 ,3 -dideoxyadenosine-5 -triphosphate in human peripheral-blood mononuclear cell by HPLC–MS/MS and the application to cell pharmacokinetics. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:337-42.  [Content Brief]

  [3]. Jun-Yu Ma, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054.  [Content Brief]

  [4]. C M Perry, et al. Didanosine. An update on its antiviral activity, pharmacokinetic properties and therapeutic efficacy in the management of HIV disease. Drugs. 1996 Dec;52(6):928-62.  [Content Brief]

  [5]. Hao Z, et al. Factors determining the activity of 2',3'-dideoxynucleosides in suppressing human immunodeficiency virus in vitro. Mol Pharmacol. 1988 Oct;34(4):431-5.  [Content Brief]