ddATP trisodium solution (100 mM)
Based on 1 publication(s) in Google Scholar
ddATP (2',3'-Dideoxyadenosine 5'-triphosphate) trisodium, an active metabolite of 2',3'-dideoxyinosine, is a chain-elongating inhibitor of DNA polymerase. ddATP trisodium can be used for Sanger method for DNA sequencing and research of virus infection.
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- Pureza : 98.86%
- No. CAS: 72029-21-1
- Fòrmula: C10H13N5Na3O11P3
- Peso molecular:541.13
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Almacenamiento:
Solution, -20°C, 2 years
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ddATP trisodium solution (100 mM)
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Actividad biológica
Descripciòn
IC50 & Target
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DNA Polymerase |
In Vitro
ddATP (0.05-50 μM, 30 min) trisodium inhibits adenovirus DNA synthesis in isolated nuclei from adenovirus-infected KB cells[1].
ddATP (100 μM, 5 h) trisodium reduces the number of γH2A.X foci in the DSB (DNA double-strand breaks) oocytes[3].
ddATP trisodium inhibits HIV replication by inhibiting HIV reverse transcriptase[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:DSB oocytes
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Concentration:100 μM
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Incubation Time:5 h
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Result:Suppressed the mtDNA replication without fully suppressed the nuclear DNA replication.
Chemical Information
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No. CAS 72029-21-1
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Appearance Liquid
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Peso molecular 541.13
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Fòrmula C10H13N5Na3O11P3
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Color Colorless to light yellow
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3CC[C@H](O3)COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O
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Synonyms
2',3'-Dideoxyadenosine 5'-triphosphate trisodium
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Solution, -20°C, 2 years
Publications (1)
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Journal Impact Factor
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Most Recent
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RNA
Improved precision, sensitivity, and adaptability of Ordered Two-Template Relay cDNA library preparation for RNA sequencing. [Abstract]2025 Jan 22;31(2):224-244. PMID: 39626888
Solvente y solubilidad
In Vitro:
H2O : 5 mg/mL (9.24 mM; Need ultrasonic)
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. van der Vliet PC, et al. Role of DNA polymerase gamma in adenovirus DNA replication. Mechanism of inhibition by 2',3'-dideoxynucleoside 5'-triphosphates. Biochemistry. 1981 Apr 28;20(9):2628-32. [Content Brief]
[2]. Xu Lan, et al. Simultaneous determination of 2 ,3 -dideoxyinosine and the active metabolite, 2 ,3 -dideoxyadenosine-5 -triphosphate in human peripheral-blood mononuclear cell by HPLC–MS/MS and the application to cell pharmacokinetics. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:337-42. [Content Brief]
[3]. Jun-Yu Ma, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054. [Content Brief]
[4]. C M Perry, et al. Didanosine. An update on its antiviral activity, pharmacokinetic properties and therapeutic efficacy in the management of HIV disease. Drugs. 1996 Dec;52(6):928-62. [Content Brief]
[5]. Hao Z, et al. Factors determining the activity of 2',3'-dideoxynucleosides in suppressing human immunodeficiency virus in vitro. Mol Pharmacol. 1988 Oct;34(4):431-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8480 mL | 9.2399 mL | 18.4798 mL | 46.1996 mL |
| 5 mM | 0.3696 mL | 1.8480 mL | 3.6960 mL | 9.2399 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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