ddATP
Based on 1 publication(s) in Google Scholar
ddATP (2',3'-Dideoxyadenosine 5'-triphosphate), an active metabolite of 2',3'-dideoxyinosine, is a chain-elongating inhibitor of DNA polymerase. ddATP can be used for Sanger method for DNA sequencing and research of virus infection.
The free form of the compound is prone to instability, it is advisable to consider the stable salt form (ddATP trisodium solution (100 mM)) that retains the same biological activity.
For research use only. We do not sell to patients.
- CAS No.: 24027-80-3
- Formula: C10H16N5O11P3
- Molecular Weight:475.18
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Storage:
Solution, -20°C, 2 years
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ddATP
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
Description
IC50 & Target
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DNA Polymerase |
In Vitro
ddATP (0.05-50 μM, 30 min) inhibits adenovirus DNA synthesis in isolated nuclei from adenovirus-infected KB cells[1].
ddATP (100 μM, 5 h) reduces the number of γH2A.X foci in the DSB (DNA double-strand breaks) oocytes[3].
ddATP inhibits HIV replication by inhibiting HIV reverse transcriptase[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:DSB oocytes
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Concentration:100 μM
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Incubation Time:5 h
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Result:Suppressed the mtDNA replication without fully suppressed the nuclear DNA replication.
Chemical Information
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CAS No. 24027-80-3
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Appearance Liquid
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Molecular Weight 475.18
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Formula C10H16N5O11P3
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Color Colorless to light yellow
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3CC[C@H](O3)COP(O)(OP(OP(O)(O)=O)(O)=O)=O
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Synonyms
2',3'-Dideoxyadenosine 5'-triphosphate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Publications (1)
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Journal Impact Factor
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Most Recent
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RNA
Improved precision, sensitivity, and adaptability of Ordered Two-Template Relay cDNA library preparation for RNA sequencing. [Abstract]2025 Jan 22;31(2):224-244. PMID: 39626888
Purity & Documentation
References
[1]. van der Vliet PC, et al. Role of DNA polymerase gamma in adenovirus DNA replication. Mechanism of inhibition by 2',3'-dideoxynucleoside 5'-triphosphates. Biochemistry. 1981 Apr 28;20(9):2628-32. [Content Brief]
[2]. Xu Lan, et al. Simultaneous determination of 2 ,3 -dideoxyinosine and the active metabolite, 2 ,3 -dideoxyadenosine-5 -triphosphate in human peripheral-blood mononuclear cell by HPLC–MS/MS and the application to cell pharmacokinetics. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:337-42. [Content Brief]
[3]. Jun-Yu Ma, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054. [Content Brief]
[4]. C M Perry, et al. Didanosine. An update on its antiviral activity, pharmacokinetic properties and therapeutic efficacy in the management of HIV disease. Drugs. 1996 Dec;52(6):928-62. [Content Brief]
[5]. Hao Z, et al. Factors determining the activity of 2',3'-dideoxynucleosides in suppressing human immunodeficiency virus in vitro. Mol Pharmacol. 1988 Oct;34(4):431-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ddATP Related Classifications
HY-128036 Related Classifications
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