HIV Reverse Transcriptase
HIV reverse transcriptase is a crucial enzyme encoded by the retroviral genome that catalyzes the conversion of viral RNA into double-stranded proviral DNA, enabling integration into the host cell's genome. As an RNA-dependent DNA polymerase, it synthesizes a DNA-RNA hybrid using the viral RNA as a template, followed by degradation of the RNA strand by RNase H and replacement with DNA to form a double-stranded DNA molecule. This proviral DNA can then integrate into the host chromosome, becoming a permanent part of the host's genetic material and serving as a template for viral mRNA production. The enzyme lacks proofreading ability due to the absence of 3′ to 5′ exonuclease activity, resulting in a high mutation rate that contributes to the genetic diversity and adaptability of HIV, facilitating immune evasion and resistance to antiretroviral drugs.
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HIV Reverse Transcriptase (1)
(-)-Calanolide B
- Formula: C22H26O5
- Molecular Weight: 370.44
(-)-Calanolide B is a non-nucleoside reverse transcriptase (NNRT) inhibitor with an IC50 of 0.5 mM against HIV-1 RT. (-)-Calanolide B inhibits the replication of HIV-1 IIIb/LAV. (-)-Calanolide B is applicable to research related to HIV-1 infection.
