GRL0617

Name: GRL0617
Brand: MedChemExpress
SKU: HY-117043
Rating: 5.0 (13 reviews)

製品番号: HY-117043 純度: 99.62%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

GRL0617 is a selective and competitive noncovalent inhibitor of severe acute respiratory syndrome (SARS-CoV) papain-like protease (PLpro), with an IC₅₀ of 0.6 μM and a Ki value of 0.49 μM. GRL0617 also inhibits SARS-CoV with an EC₅₀ of 14.5 μM. GRL0617 can be used for the research of severe acute respiratory syndrome.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

GRL0617 構造式

CAS 番号 : 1093070-16-6

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 99	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 99	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$90	在庫あり	Estimated Time of Arrival: December 31
10 mg	$140	在庫あり	Estimated Time of Arrival: December 31
25 mg	$260	在庫あり	Estimated Time of Arrival: December 31
50 mg	$420	在庫あり	Estimated Time of Arrival: December 31
100 mg	$670	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 13 publication(s) in Google Scholar

顧客検証

•ACS Infect Dis. 2022 Mar 11;8(3):596-611. [Abstract]

Deubiquitinase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15 USP28 USP25

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

IC50: 0.6 μM (SARS-CoV PLpro)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	CC₅₀	500 3 Compound: 4; GRL0617	Cytotoxicity against African green monkey Vero cells treated for 24 hrs by CCK8 assay Cytotoxicity against African green monkey Vero cells treated for 24 hrs by CCK8 assay	[PMID: 35620927]
Vero	EC₅₀	13.1 3 Compound: 25	Antiviral activity against SARS coronavirus in african green monkey Vero E6 cells assessed as inhibition of replication after 48 hrs Antiviral activity against SARS coronavirus in african green monkey Vero E6 cells assessed as inhibition of replication after 48 hrs	[PMID: 19645480]
Vero	EC₅₀	14.5 3 Compound: GRL0617	Antiviral activity against SARS-CoV infected in african green monkey Vero E6 cells at 0.1 to 50 uM after 48 hrs Antiviral activity against SARS-CoV infected in african green monkey Vero E6 cells at 0.1 to 50 uM after 48 hrs	[PMID: 23231439]
Vero	EC₅₀	13.1 3 Compound: 25	Antiviral activity against SARS coronavirus in african green monkey Vero E6 cells assessed as inhibition of replication after 48 hrs Antiviral activity against SARS coronavirus in african green monkey Vero E6 cells assessed as inhibition of replication after 48 hrs	[PMID: 19645480]
Vero	CC₅₀	500 3 Compound: 4; GRL0617	Cytotoxicity against African green monkey Vero cells treated for 24 hrs by CCK8 assay Cytotoxicity against African green monkey Vero cells treated for 24 hrs by CCK8 assay	[PMID: 35620927]
Vero	EC₅₀	14.5 3 Compound: GRL0617	Antiviral activity against SARS-CoV infected in african green monkey Vero E6 cells at 0.1 to 50 uM after 48 hrs Antiviral activity against SARS-CoV infected in african green monkey Vero E6 cells at 0.1 to 50 uM after 48 hrs	[PMID: 23231439]
Vero C1008	EC₅₀	10 3 Compound: 5	Antiviral activity against SARS coronavirus Urbani infected in monkey Vero E6 cells assessed as reduction in virus-induced cytopathogenicity incubated for 48 hrs by CellTiter-Glo luminescent cell viability assay Antiviral activity against SARS coronavirus Urbani infected in monkey Vero E6 cells assessed as reduction in virus-induced cytopathogenicity incubated for 48 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 32665808]
Vero	CC₅₀	500 3 Compound: 4; GRL0617	Cytotoxicity against African green monkey Vero cells treated for 24 hrs by CCK8 assay Cytotoxicity against African green monkey Vero cells treated for 24 hrs by CCK8 assay	[PMID: 35620927]
Vero	EC₅₀	13.1 3 Compound: 25	Antiviral activity against SARS coronavirus in african green monkey Vero E6 cells assessed as inhibition of replication after 48 hrs Antiviral activity against SARS coronavirus in african green monkey Vero E6 cells assessed as inhibition of replication after 48 hrs	[PMID: 19645480]
Vero	EC₅₀	14.5 3 Compound: GRL0617	Antiviral activity against SARS-CoV infected in african green monkey Vero E6 cells at 0.1 to 50 uM after 48 hrs Antiviral activity against SARS-CoV infected in african green monkey Vero E6 cells at 0.1 to 50 uM after 48 hrs	[PMID: 23231439]
Vero C1008	CC₅₀	253.2 3 Compound: GRL0617	Cytotoxicity against african green monkey Vero E6 cells incubated for 48 hrs Cytotoxicity against african green monkey Vero E6 cells incubated for 48 hrs	[PMID: 38871484]
Vero C1008	EC₅₀	10 3 Compound: 5	Antiviral activity against SARS coronavirus Urbani infected in monkey Vero E6 cells assessed as reduction in virus-induced cytopathogenicity incubated for 48 hrs by CellTiter-Glo luminescent cell viability assay Antiviral activity against SARS coronavirus Urbani infected in monkey Vero E6 cells assessed as reduction in virus-induced cytopathogenicity incubated for 48 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 32665808]

体外実験

GRL0617 (0-50 μM; 48 h) exibits significantly antiviral activity in SARS-CoV infected Vero E6 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Vero E6 cells
Concentration:	0, 10, 20, 30, 40 and 50 μM
Incubation Time:	48 hours
Result:	Showed significant antiviral activity in SARS-CoV infected Vero E6 cells with an EC₅₀ value of 14.5 μM.

分子量

304.39

分子式

C₂₀H₂₀N₂O

CAS 番号

1093070-16-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N[C@@H](C1=C2C=CC=CC2=CC=C1)C)C3=CC(N)=CC=C3C

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 125 mg/mL (410.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2853 mL	16.4263 mL	32.8526 mL
5 mM	0.6571 mL	3.2853 mL	6.5705 mL
10 mM	0.3285 mL	1.6426 mL	3.2853 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 2.5 mg/mL (8.21 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.62%

Select Batch:

データシート (287 KB) SDS (393 KB)

COA (251 KB) LCMS (94 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Ratia K, et al. A noncovalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16119-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2853 mL	16.4263 mL	32.8526 mL	82.1315 mL
	5 mM	0.6571 mL	3.2853 mL	6.5705 mL	16.4263 mL
	10 mM	0.3285 mL	1.6426 mL	3.2853 mL	8.2131 mL
	15 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4754 mL
	20 mM	0.1643 mL	0.8213 mL	1.6426 mL	4.1066 mL
	25 mM	0.1314 mL	0.6571 mL	1.3141 mL	3.2853 mL
	30 mM	0.1095 mL	0.5475 mL	1.0951 mL	2.7377 mL
	40 mM	0.0821 mL	0.4107 mL	0.8213 mL	2.0533 mL
	50 mM	0.0657 mL	0.3285 mL	0.6571 mL	1.6426 mL
	60 mM	0.0548 mL	0.2738 mL	0.5475 mL	1.3689 mL
	80 mM	0.0411 mL	0.2053 mL	0.4107 mL	1.0266 mL
	100 mM	0.0329 mL	0.1643 mL	0.3285 mL	0.8213 mL

GRL0617 Related Classifications

Infection

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GRL06171093070-16-6GRL 0617GRL-0617DeubiquitinaseSARS-CoVDUBsSARS coronaviruscompetitive noncovalent inhibitorInhibitorinhibitorinhibit

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Deubiquitinase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

GRL0617 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Deubiquitinase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

GRL0617 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

GRL0617 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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