Ribonucleoside vanadyl complexes 

Ribonucleoside vanadyl complexes are a class of potent RNase and Taq polymerase inhibitors. Ribonucleoside vanadyl complexes protect RNA during RNA isolation by inhibiting ribonucleases, and also reduce the viability of bacteria and eukaryotic cells by interfering with ribosomal subunit assembly. Ribonucleoside vanadyl complexes block PCR and reverse transcription reactions templated by viral nucleic acids and enhance the effects of antibiotics against Staphylococcus aureus, but do not directly inhibit protein synthesis. Ribonucleoside vanadyl complexes can be effectively removed by phenol-chloroform extraction, thus enabling subsequent PCR analysis. Ribonucleoside vanadyl complexes can be applied in research related to chronic hepatitis C (HCV) and Staphylococcus aureus infection^[1][2].

Ribonucleoside vanadyl complexes (RVC) (0.04-8 mM) potently inhibit PCR reactions using both HCV cDNA and HBV DNA templates, with consistent inhibition observed at concentrations of 0.4 mM and higher, while 0.04 mM has no inhibitory effect^[1].
Ribonucleoside vanadyl complexes (RVC) (20 mM; 4-5 days) inhibits RT-nPCR detection of HCV RNA, even when positive HCV RNA is spiked into the reaction, when included during prolonged proteinase K digestion of formalin-fixed paraffin-embedded liver tissue^[1].
Ribonucleoside vanadyl complexes (5 mM) reduced the viable cell counts of methicillin-resistant (HY-121544) MSSA RN1786 and MRSA A1024 by >90%^[2].
Ribonucleoside vanadyl complexes (5 mM) reduces ribosomal subunit formation by 90%, reduces 50S subunit relative abundance by ~15% in MSSA cells, and increases rRNA degradation in both MSSA RN1786 and MRSA A1024 cells^[2].
Ribonucleoside vanadyl complexes (5 mM) reduces the synthesis rate of both 30S and 50S ribosomal subunits, including a 4-fold reduction in 50S subunit synthesis, in MSSA RN1786 and MRSA A1024 cells^[2].
Ribonucleoside vanadyl complexes (1-5 mM; ~34 h) reduce viable cell counts by ~90%-98% in RAW 264.7 macrophage and BJ fibroblast cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Dark blue to black

[Ribonucleoside vanadyl complexes]

Room temperature in continental US; may vary elsewhere.

• [1]. Lau J Y, et al. Ribonucleotide vanadyl complexes inhibit polymerase chain reaction[J]. Nucleic acids research, 1993, 21(11): 2777.

  [2]. Frazier AD, et al. The vanadyl ribonucleoside complex inhibits ribosomal subunit formation in Staphylococcus aureus. J Antimicrob Chemother. 2012;67(9):2152-2157.  [Content Brief]