2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease
  3. Opioid Receptor Endogenous Metabolite
  4. [Leu5]-Enkephalin

[Leu5]-Enkephalin  (Synonyms: Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin)

製品番号: HY-P0288 純度: 99.92%
COA 取扱説明書 Technical Support

[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

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カスタムペプチド

CAS 番号 : 58822-25-6

容量 価格（税別） 在庫状況 数量
5 mg $30 在庫あり
10 mg $50 在庫あり
25 mg $95 在庫あり
50 mg $142 在庫あり
100 mg $200 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of [Leu5]-Enkephalin:

[Leu5]-Enkephalin 関連抗体

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
Cell Imaging/Staining
WB

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    [Leu5]-Enkephalin (1-200 μM; 24 h) did not exhibit cytotoxicity in ARPE-19 cells (P > 0.05).

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    [Leu5]-Enkephalin (10-30 µM; 24 h) significantly ameliorated the OGD-induced reduction in ARPE-19 cell viability.

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    Impacts of naltrindole and enkephalins treatment on cell viability, morphological changes, and apoptosis rates in OGD/R-injured ARPE-19 cells (mean ± SEM, n = 4 or 5 replicates per group, scale bar = 200 µm). The results showed that following treatment with [Leu5]-Enkephalin (MEK), the cell count increased significantly, and cell morphology reverted to a normal state.

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    Effects of naloxone and enkephalin on DOR expression in total cellular and membrane protein fractions of ARPE-19 cells under OGD/R (mean ± SEM, n = 4 independent experiments). The results showed that treatment with [Leu5]-Enkephalin (MEK) increased DOR levels in both total proteins (P <0.05) and membrane proteins (P < 0.001).

    Opioid Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    μ Opioid Receptor/MOR κ Opioid Receptor/KOR δ Opioid Receptor/DOR NOP Receptor/ORL1

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    [Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CHO EC50
    0.08 nM
    Compound: 105
    Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells
    Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 31377035]
    CHO EC50
    1.3 nM
    Compound: 105
    Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells
    Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 31377035]
    CHO EC50
    80 nM
    Compound: 105
    Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells
    Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 31377035]
    CHO EC50
    80 nM
    Compound: 283
    Activation of kappa opioid receptor (unknown origin) expressed in CHO cells
    Activation of kappa opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 29400967]
    体外実験

    Enkephalins (met- , leu-enkephalin, and enkephalin 8) and dynorphins are two classes of opioid peptides found in the spinal dorsal horn. Mu, delta, and kappa are three major subtypes of opioid receptors. Enkephalins are putative endogenous ligands for delta opioid receptors, and dynorphins are endogenous ligands for the kappa opioid receptors. Three receptor types resembling the vertebrate δ- and κ-type opioid receptors have been characterized pharmacologically in nervous tissues (e.g. Ki=18.9 nM for Leu-enkephalin) and localized by autoradiography at CHH terminals in the SG of C. maenas[1].
    Leucine-enkephalin is a pentapeptides with morphine like properties, naturally present in mammalian brain[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    [Leu5]-Enkephalin 関連抗体
    分子量

    555.62

    分子式

    C28H37N5O7
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    Sequence

    Tyr-Gly-Gly-Phe-Leu
    Sequence Shortening

    YGGFL
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Sealed storage, away from moisture
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 116.67 mg/mL (209.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (179.98 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7998 mL 8.9990 mL 17.9979 mL
    5 mM 0.3600 mL 1.7998 mL 3.5996 mL
    10 mM 0.1800 mL 0.8999 mL 1.7998 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 6.67 mg/mL (12.00 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.92%

    COA (245 KB) RP-HPLC (251 KB) MS (68 KB) Elemental Analysis Report (104 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.7998 mL 8.9990 mL 17.9979 mL 44.9948 mL
    5 mM 0.3600 mL 1.7998 mL 3.5996 mL 8.9990 mL
    10 mM 0.1800 mL 0.8999 mL 1.7998 mL 4.4995 mL
    15 mM 0.1200 mL 0.5999 mL 1.1999 mL 2.9997 mL
    20 mM 0.0900 mL 0.4499 mL 0.8999 mL 2.2497 mL
    25 mM 0.0720 mL 0.3600 mL 0.7199 mL 1.7998 mL
    30 mM 0.0600 mL 0.3000 mL 0.5999 mL 1.4998 mL
    40 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1249 mL
    50 mM 0.0360 mL 0.1800 mL 0.3600 mL 0.8999 mL
    60 mM 0.0300 mL 0.1500 mL 0.3000 mL 0.7499 mL
    80 mM 0.0225 mL 0.1125 mL 0.2250 mL 0.5624 mL
    100 mM 0.0180 mL 0.0900 mL 0.1800 mL 0.4499 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    [Leu5]-Enkephalin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    [Leu5]-Enkephalin58822-25-6Leu-enkephalin Leucine enkephalin Leucyl-enkephalinOpioid ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-P0288
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