[Leu5]-Enkephalin (Synonyms: Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin)

Name: [Leu5]-Enkephalin
Brand: MedChemExpress
SKU: HY-P0288
Rating: 5.0 (2 reviews)

Cat. No.: HY-P0288 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Custom Peptide Synthesis

CAS No. : 58822-25-6

사이즈	재고	수량
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 2 publication(s) in Google Scholar

Other Forms of [Leu5]-Enkephalin:

[Leu5]-Enkephalin TFA 견적 받기

2 Publications Citing Use of MCE [Leu5]-Enkephalin

Cell Proliferation/Viability Assay

Cell Imaging/Staining

: [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.; [Leu5]-Enkephalin (1-200 μM; 24 h) did not exhibit cytotoxicity in ARPE-19 cells (P > 0.05).

: [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.; [Leu5]-Enkephalin (10-30 µM; 24 h) significantly ameliorated the OGD-induced reduction in ARPE-19 cell viability.

: [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.; Impacts of naltrindole and enkephalins treatment on cell viability, morphological changes, and apoptosis rates in OGD/R-injured ARPE-19 cells (mean ± SEM, n = 4 or 5 replicates per group, scale bar = 200 µm). The results showed that following treatment with [Leu5]-Enkephalin (MEK), the cell count increased significantly, and cell morphology reverted to a normal state.

: [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.; Effects of naloxone and enkephalin on DOR expression in total cellular and membrane protein fractions of ARPE-19 cells under OGD/R (mean ± SEM, n = 4 independent experiments). The results showed that treatment with [Leu5]-Enkephalin (MEK) increased DOR levels in both total proteins (P <0.05) and membrane proteins (P < 0.001).

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μ Opioid Receptor/MOR κ Opioid Receptor/KOR δ Opioid Receptor/DOR NOP Receptor/ORL1

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

Biological Activity
순도&문서
References
고객리뷰

제품 설명

[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.08 1 Compound: 105	Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	0.08 1 Compound: 105	Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	1.3 1 Compound: 105	Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	1.3 1 Compound: 105	Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	80 1 Compound: 105	Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	80 1 Compound: 283	Activation of kappa opioid receptor (unknown origin) expressed in CHO cells Activation of kappa opioid receptor (unknown origin) expressed in CHO cells	[PMID: 29400967]
CHO	EC₅₀	80 1 Compound: 283	Activation of kappa opioid receptor (unknown origin) expressed in CHO cells Activation of kappa opioid receptor (unknown origin) expressed in CHO cells	[PMID: 29400967]
CHO	EC₅₀	80 1 Compound: 105	Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]

In Vitro

Enkephalins (met- , leu-enkephalin, and enkephalin 8) and dynorphins are two classes of opioid peptides found in the spinal dorsal horn. Mu, delta, and kappa are three major subtypes of opioid receptors. Enkephalins are putative endogenous ligands for delta opioid receptors, and dynorphins are endogenous ligands for the kappa opioid receptors. Three receptor types resembling the vertebrate δ- and κ-type opioid receptors have been characterized pharmacologically in nervous tissues (e.g. K_i=18.9 nM for Leu-enkephalin) and localized by autoradiography at CHH terminals in the SG of C. maenas^[1].
Leucine-enkephalin is a pentapeptides with morphine like properties, naturally present in mammalian brain^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

555.62

화학식

C₂₈H₃₇N₅O₇

CAS No.

58822-25-6

Appearance

Solid

Color

White to light yellow

Sequence

Tyr-Gly-Gly-Phe-Leu

Sequence Shortening

YGGFL

Structure Classification

Others

Initial Source

Endogenous metabolite

선적

Room temperature in continental US; may vary elsewhere.

보관

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도

In Vitro:

DMSO : 116.67 mg/mL (209.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (179.98 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7998 mL	8.9990 mL	17.9979 mL
5 mM	0.3600 mL	1.7998 mL	3.5996 mL
10 mM	0.1800 mL	0.8999 mL	1.7998 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 6.67 mg/mL (12.00 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.92%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (245 KB) RP-HPLC (251 KB) MS (68 KB) Elemental Analysis Report (104 KB)

Handling Instructions (2659 KB)

References

[1]. Leu-enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

[2]. Meunier JC, et al. Binding of Leu5-enkephalin and Met5-enkephalin to a particulate fraction from rat cerebrum. FEBS Lett. 1977 May 15;77(2):209-13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7998 mL	8.9990 mL	17.9979 mL	44.9948 mL
	5 mM	0.3600 mL	1.7998 mL	3.5996 mL	8.9990 mL
	10 mM	0.1800 mL	0.8999 mL	1.7998 mL	4.4995 mL
	15 mM	0.1200 mL	0.5999 mL	1.1999 mL	2.9997 mL
	20 mM	0.0900 mL	0.4499 mL	0.8999 mL	2.2497 mL
	25 mM	0.0720 mL	0.3600 mL	0.7199 mL	1.7998 mL
	30 mM	0.0600 mL	0.3000 mL	0.5999 mL	1.4998 mL
	40 mM	0.0450 mL	0.2250 mL	0.4499 mL	1.1249 mL
	50 mM	0.0360 mL	0.1800 mL	0.3600 mL	0.8999 mL
	60 mM	0.0300 mL	0.1500 mL	0.3000 mL	0.7499 mL
	80 mM	0.0225 mL	0.1125 mL	0.2250 mL	0.5624 mL
	100 mM	0.0180 mL	0.0900 mL	0.1800 mL	0.4499 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

[Leu5]-Enkephalin Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

[Leu5]-Enkephalin58822-25-6Leu-enkephalin Leucine enkephalin Leucyl-enkephalinOpioid ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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[Leu5]-Enkephalin (Synonyms: Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin)

고객리뷰

Other Forms of [Leu5]-Enkephalin:

[Leu5]-Enkephalin Related Antibodies

2 Publications Citing Use of MCE [Leu5]-Enkephalin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Opioid Receptor Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

[Leu5]-Enkephalin Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

[Leu5]-Enkephalin Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report