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  4. 7,8-Dihydro-L-biopterin

7,8-Dihydro-L-biopterin  (Synonyms: 7,8-ジヒドロ-L-ビオプテリン)

製品番号: HY-W008646 純度: 99.79%
COA 取扱説明書 Technical Support

7,8-Dihydro-L-biopterin is a NOS uncoupling inducer with blood-brain barrier permeability, and it is a reduced non-conjugated pteridine. 7,8-Dihydro-L-biopterin is the main metabolite of 4-amino-tetrahydro-L-biopterin, and it undergoes photooxidation to form biopterin. 7,8-Dihydro-L-biopterin promotes the conversion of nitric oxide synthase to a superoxide-producing form, thereby increasing oxidative stress levels in the renal outer medulla and inducing apoptosis. 7,8-Dihydro-L-biopterin is sensitive to the inhibitory effect of SOD, and it can be applied to research related to salt-sensitive hypertension, moderate to severe traumatic brain injury, and neurodegenerative diseases.

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7,8-Dihydro-L-biopterin

7,8-Dihydro-L-biopterin 構造式

CAS 番号 : 6779-87-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 在庫あり
Solution
10 mM * 1 mL in DMSO USD 82 在庫あり
Solid
5 mg $75 在庫あり
10 mg $110 在庫あり
25 mg $220 在庫あり
50 mg $360 在庫あり
100 mg $580 在庫あり
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Based on 1 publication(s) in Google Scholar

Other Forms of 7,8-Dihydro-L-biopterin:

7,8-Dihydro-L-biopterin 関連抗体

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製品説明

7,8-Dihydro-L-biopterin is a NOS uncoupling inducer with blood-brain barrier permeability, and it is a reduced non-conjugated pteridine. 7,8-Dihydro-L-biopterin is the main metabolite of 4-amino-tetrahydro-L-biopterin, and it undergoes photooxidation to form biopterin. 7,8-Dihydro-L-biopterin promotes the conversion of nitric oxide synthase to a superoxide-producing form, thereby increasing oxidative stress levels in the renal outer medulla and inducing apoptosis. 7,8-Dihydro-L-biopterin is sensitive to the inhibitory effect of SOD, and it can be applied to research related to salt-sensitive hypertension, moderate to severe traumatic brain injury, and neurodegenerative diseases[1][2][3][4].

IC50 & Target

Human Endogenous Metabolite

 

体外実験

7,8-Dihydro-L-biopterin (200 ng/mL) spontaneously oxidizes such that biopterin is always present in the solution[3].
7,8-Dihydro-L-biopterin (50-500 ng/mL; 20 min UV-irradiation) in aqueous solution can be quantified via off-line UV photoirradiation followed by HPLC-fluorimetric detection with a limit of detection of 24 ng/mL (peak height) or 35 ng/mL (peak area)[3].
7,8-Dihydro-L-biopterin (10 μM-5 mM; 48 h) induces a 1.7-3.8-fold increase in apoptosis in non-differentiated PC12 rat pheochromocytoma cells, and this effect is partially inhibited by SOD[4].
7,8-Dihydro-L-biopterin shows no significant apoptosis induction in NGF-differentiated PC12 rat pheochromocytoma cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7,8-Dihydro-L-biopterin 関連抗体

Apoptosis Analysis[4]

Cell Line: Non-differentiated PC12 rat pheochromocytoma cells
Concentration: 10 μM, 200 μM, 1 mM, 5 mM
Incubation Time: 48 h
Result: Induced a 1.7-3.8-fold increase in apoptosis compared to control.
Showed characteristic apoptotic features including chromatin condensation and apoptotic bodies via Hoechst 33342 staining.
Was inhibited 25% by superoxide dismutase (SOD), while catalase showed no inhibitory effect.
体内実験

Compared with SS-13BN rats fed a normal-salt diet or a high-salt diet, the level of 7,8-Dihydro-L-biopterin in the renal outer medulla of SS rats is significantly elevated, resulting in a decreased BH4/BH2 ratio, which is consistent with the characteristic of nitric oxide synthase uncoupling in hypertensive SS rats[1].
NOS uncoupling inducers primarily function to disrupt the normal activity of nitric oxide synthase (NOS), transforming it from a beneficial "protector" into a harmful "damager". NOS uncoupling inducers drive NOS to cease producing the beneficial nitric oxide (NO) for the body and instead generate large amounts of cytotoxic superoxide (O2-), thereby triggering oxidative stress and cellular damage[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

239.23

分子式

C9H13N5O3
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N=C(N)NC2=C1N=C([C@@H](O)[C@@H](O)C)CN2
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 150 mg/mL (627.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (20.90 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1801 mL 20.9004 mL 41.8008 mL
5 mM 0.8360 mL 4.1801 mL 8.3602 mL
10 mM 0.4180 mL 2.0900 mL 4.1801 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

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体積 （開始）

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 12.5 mg/mL (52.25 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.1801 mL 20.9004 mL 41.8008 mL 104.5019 mL
5 mM 0.8360 mL 4.1801 mL 8.3602 mL 20.9004 mL
10 mM 0.4180 mL 2.0900 mL 4.1801 mL 10.4502 mL
15 mM 0.2787 mL 1.3934 mL 2.7867 mL 6.9668 mL
20 mM 0.2090 mL 1.0450 mL 2.0900 mL 5.2251 mL
DMSO 25 mM 0.1672 mL 0.8360 mL 1.6720 mL 4.1801 mL
30 mM 0.1393 mL 0.6967 mL 1.3934 mL 3.4834 mL
40 mM 0.1045 mL 0.5225 mL 1.0450 mL 2.6125 mL
50 mM 0.0836 mL 0.4180 mL 0.8360 mL 2.0900 mL
60 mM 0.0697 mL 0.3483 mL 0.6967 mL 1.7417 mL
80 mM 0.0523 mL 0.2613 mL 0.5225 mL 1.3063 mL
100 mM 0.0418 mL 0.2090 mL 0.4180 mL 1.0450 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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7,8-Dihydro-L-biopterin Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7,8-Dihydro-L-biopterin6779-87-9SODApoptosisNO SynthaseSuperoxide DismutaseNitric oxide synthasesNOSnitric oxide synthasePC12 rat pheochromocytoma cellsDahl salt-sensitive ratssuperoxide dismutaserenal outer medullasuperoxide4-amino-tetrahydro-L-biopterinapoptosissalt-sensitive hypertensionbiopterinInhibitorinhibitorinhibit

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