2-Ketoglutaric acid (Synonyms: Alpha-Ketoglutaric acid; 2-Oxoglutaric acid)

Name: 2-Ketoglutaric acid
Brand: MedChemExpress
SKU: HY-W013636
Rating: 5.0 (6 reviews)

Cat. No.: HY-W013636 Pureza: 99.68%: Ficha de datos
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2-Ketoglutaric acid (Alpha-Ketoglutaric acid) is an intermediate in the production of ATP or GTP in the Krebs cycle. 2-Ketoglutaric acid also acts as the major carbon skeleton for nitrogen-assimilatory reactions. 2-Ketoglutaric acid is a reversible inhibitor of tyrosinase (IC₅₀=15 mM).

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No. CAS : 328-50-7

Tamaño	Stock	Cantidad
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	Obtener un presupuesto
10 g	Obtener un presupuesto

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Revisión del cliente

Based on 6 publication(s) in Google Scholar

Other Forms of 2-Ketoglutaric acid:

2-Ketoglutaric acid-¹³C₅ In-stock
2-Ketoglutaric acid-d₄ In-stock
2-Ketoglutaric acid-d₆ In-stock
2-Ketoglutaric acid-¹³C In-stock
2-Ketoglutaric acid Sodium In-stock
Calcium 2-oxoglutarate In-stock
2-Ketoglutaric acid potassium In-stock
2-Ketoglutaric acid (Standard) In-stock
2-Ketoglutaric acid-¹³C₂ Obtener un presupuesto

6 Publications Citing Use of MCE 2-Ketoglutaric acid

Cell Proliferation/Viability Assay

: 2-Ketoglutaric acid purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 May:71:103112. [Abstract]; Cells were infected with PDCoV in Gln-containing medium, Gln-free medium, or Gln-free medium supplemented with oxoglutaric acid (αKG) (1 or 4 mM).

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Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todas las isoformas

Human Endogenous Metabolite Microbial Metabolite

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Referencias
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Descripciòn

IC₅₀ & Target^[1]

Human Endogenous Metabolite

Microbial Metabolite

Tyrosinase

15 mM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	54 3 Compound: 164533	Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells	10.5281/zenodo.7360495
HEK293	EC₅₀	54 3 Compound: 164533	Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells (PubChem AID: 1794820) Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells (PubChem AID: 1794820)	10.5281/zenodo.7360495
HEK293	EC₅₀	54 3 Compound: 164533	Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells (PubChem AID: 1794820) Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells (PubChem AID: 1794820)	10.5281/zenodo.7360495
Oocyte	IC₅₀	700 3 Compound: alpha-Ketoglutarate	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes	[PMID: 11502595]
Oocyte	IC₅₀	740 3 Compound: alpha-Ketoglutarate	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes	[PMID: 11502595]
Oocyte	IC₅₀	700 3 Compound: alpha-Ketoglutarate	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes	[PMID: 11502595]
Oocyte	IC₅₀	740 3 Compound: alpha-Ketoglutarate	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of TEA uptake (TEA: 10 uM) in Xenopus laevis oocytes	[PMID: 11502595]

In Vitro

2-Ketoglutaric acid (Alpha-Ketoglutaric acid) has other physiological capabilities including reduction of ammonia level formed in the lung and general ammonia detoxification, protective role against lipid peroxidation and neuroprotective effect against cyanide poisoning^[1].
2-Ketoglutaric acid acts as precursor for the synthesis of amino acids and nucleotides^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

146.10

Fòrmula

C₅H₆O₅

No. CAS

328-50-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C(O)=O)CCC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 250 mg/mL (1711.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (342.23 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.8446 mL	34.2231 mL	68.4463 mL
5 mM	1.3689 mL	6.8446 mL	13.6893 mL
10 mM	0.6845 mL	3.4223 mL	6.8446 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (14.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (14.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (14.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (684.46 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.68%

Select Batch:

Ficha de datos (272 KB) SDS (476 KB)

COA (243 KB) HNMR (266 KB) RP-HPLC (274 KB) MS (178 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Huergo LF, et al. The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite. Microbiol Mol Biol Rev. 2015 Dec;79(4):419-35. [Content Brief]

[2]. Gou L, et al. The effect of alpha-ketoglutaric acid on tyrosinase activity and conformation: Kinetics and molecular dynamics simulation study. Int J Biol Macromol. 2017 Dec;105(Pt 3):1654-1662. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.8446 mL	34.2231 mL	68.4463 mL	171.1157 mL
	5 mM	1.3689 mL	6.8446 mL	13.6893 mL	34.2231 mL
	10 mM	0.6845 mL	3.4223 mL	6.8446 mL	17.1116 mL
	15 mM	0.4563 mL	2.2815 mL	4.5631 mL	11.4077 mL
	20 mM	0.3422 mL	1.7112 mL	3.4223 mL	8.5558 mL
	25 mM	0.2738 mL	1.3689 mL	2.7379 mL	6.8446 mL
	30 mM	0.2282 mL	1.1408 mL	2.2815 mL	5.7039 mL
	40 mM	0.1711 mL	0.8556 mL	1.7112 mL	4.2779 mL
	50 mM	0.1369 mL	0.6845 mL	1.3689 mL	3.4223 mL
	60 mM	0.1141 mL	0.5704 mL	1.1408 mL	2.8519 mL
	80 mM	0.0856 mL	0.4278 mL	0.8556 mL	2.1389 mL
	100 mM	0.0684 mL	0.3422 mL	0.6845 mL	1.7112 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2-Ketoglutaric acid Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Ketoglutaric acid328-50-7Alpha-Ketoglutaric acid 2-Oxoglutaric acidTyrosinaseEndogenous MetaboliteintermediateprecursorTCAcarbonskeletonInhibitorinhibitorinhibit

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2-Ketoglutaric acid (Synonyms: Alpha-Ketoglutaric acid; 2-Oxoglutaric acid)

Revisión del cliente

Other Forms of 2-Ketoglutaric acid:

2-Ketoglutaric acid Related Antibodies

6 Publications Citing Use of MCE 2-Ketoglutaric acid

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

2-Ketoglutaric acid Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

2-Ketoglutaric acid Related Classifications

Keywords:

Productos vistos recientemente:

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SAFETY DATA SHEET (SDS)

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