2. 疾患モデリングル製品 Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Immunology and Inflammatory Disease Models Thyroid Hormone Receptor Endogenous Metabolite
  4. Hapten
  5. Liothyronine

リオチロニン  (Synonyms: Liothyronine; Triiodothyronine; T3; )

製品番号: HY-A0070A 純度: 99.82%
COA 取扱説明書 Technical Support

Liothyronine is an active form of thyroid hormone. Liothyronine binds to thyroid hormone receptors TRα and TRβ with Kis of 2.33 and 2.29 nM for hTRα and hTRβ, respectively. Liothyronine also binds to PVR and blocks the interaction of TIGIT/PVR.

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CAS 番号 : 6893-02-3

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100 mg $60 在庫あり
500 mg $130 在庫あり
1 g $208 在庫あり
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10 g   お問い合わせ  

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カスタマーレビュー

Based on 39 publication(s) in Google Scholar

Other Forms of Liothyronine:

リオチロニン 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
Cell Proliferation/Viability Assay
Cell Migration/Invasion Assay

    Liothyronine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    PGC1α mRNA levels in TRβ‐overexpressing ccRCC cell lines versus controls following Triiodothyronine (T3) treatment (100 nM for 48 h).

    Liothyronine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    CCK8 assay showing proliferation of TRβ-overexpressing cells treated with T3 (100 nM for 48 h).

    Liothyronine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    Transwell assay results comparing the migration of TRβ-overexpressing cells with control cells in the presence of T3 (100 nM for 48 h).

    Liothyronine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8.  [Abstract]

    C3H10T1/2 preadipocytes were treated with differentiation medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nM Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days and subsequently cultured in maintaining medium supplemented with 1 nM Triiodothyronine, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were cultured in complete medium until used for experiments. Representative immunoblots of PGC-1α and UCP1 in beige adipocytes treated as indicated.

    Liothyronine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 2;14(1):3208.  [Abstract]

    C3H10T1/2 preadipocytes were cultured in DMEM supplemented with 10% FBS, 1% P/S. For adipogenic differentiation, confluent C3H10T1/2 preadipocytes were incubated with complete medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nm Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days. Then, cells were changed to the complete medium supplemented with 1 nM T3, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were maintained in complete medium until used for experiments. Representative immunoblots of NRP1 and EPHA7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Liothyronine is an active form of thyroid hormone. Liothyronine binds to thyroid hormone receptors TRα and TRβ with Kis of 2.33 and 2.29 nM for hTRα and hTRβ, respectively. Liothyronine also binds to PVR and blocks the interaction of TIGIT/PVR[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CHO EC50
    3.4 nM
    Compound: T3
    Agonist activity at human THRalpha expressed in CHO cells after 24 hrs by luciferase assay
    Agonist activity at human THRalpha expressed in CHO cells after 24 hrs by luciferase assay
    		[PMID: 32356658]
    CHO EC50
    6.2 nM
    Compound: T3
    Agonist activity at human THRbeta expressed in CHO cells after 24 hrs by luciferase assay
    Agonist activity at human THRbeta expressed in CHO cells after 24 hrs by luciferase assay
    		[PMID: 32356658]
    CHO-K1 IC50
    0.24 nM
    Compound: 1
    Binding affinity at human thyroid hormone receptor alpha 1 expressed in CHOK1 cells
    Binding affinity at human thyroid hormone receptor alpha 1 expressed in CHOK1 cells
    		[PMID: 17543524]
    CHO-K1 IC50
    0.26 nM
    Compound: 1
    Binding affinity at human thyroid hormone receptor beta-1 expressed in CHOK1 cells
    Binding affinity at human thyroid hormone receptor beta-1 expressed in CHOK1 cells
    		[PMID: 17543524]
    CV-1 EC50
    0.002 μM
    Compound: T3
    Agonist activity at human recombinant TRbeta1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay
    Agonist activity at human recombinant TRbeta1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay
    		[PMID: 24290064]
    CV-1 EC50
    0.0021 μM
    Compound: T3
    Agonist activity at human recombinant TRalpha1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay
    Agonist activity at human recombinant TRalpha1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay
    		[PMID: 24290064]
    CV-1 EC50
    0.5 nM
    Compound: T3
    Agonist activity at Xenopus laevis THRalpha transfected in green monkey CV1 cells after 48 hrs by reporter gene assay
    Agonist activity at Xenopus laevis THRalpha transfected in green monkey CV1 cells after 48 hrs by reporter gene assay
    		[PMID: 32356658]
    CV-1 EC50
    1.2 nM
    Compound: T3
    Agonist activity at human THRbeta expressed in green monkey CV1 cells after 24 hrs by luciferase assay
    Agonist activity at human THRbeta expressed in green monkey CV1 cells after 24 hrs by luciferase assay
    		[PMID: 32356658]
    HEK293 EC50
    0.41 nM
    Compound: T3
    Agonist activity at human thyroid hormone receptor alpha expressed in HEK293 cells by luciferase reporter gene assay
    Agonist activity at human thyroid hormone receptor alpha expressed in HEK293 cells by luciferase reporter gene assay
    		[PMID: 25305688]
    HEK-293T EC50
    5.8 μM
    Compound: T3
    Agonist activity at recombinant human pFA-CMV fused PPARgamma expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay
    Agonist activity at recombinant human pFA-CMV fused PPARgamma expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay
    		[PMID: 32356658]
    HeLa EC50
    2.4 nM
    Compound: T3
    Effect on human TRalpha transactivation activity in HeLa cells by luciferase reporter assay
    Effect on human TRalpha transactivation activity in HeLa cells by luciferase reporter assay
    		[PMID: 17988877]
    HeLa EC50
    2.4 nM
    Compound: T3
    Effect on human TRbeta transactivation activity in HeLa cells by luciferase reporter assay
    Effect on human TRbeta transactivation activity in HeLa cells by luciferase reporter assay
    		[PMID: 17988877]
    LNCaP IC50
    >50 μM
    Compound: 6, T3
    Transcriptional activity at human androgen receptor BF3 site stably transfected in eGFP-expressing human LNCAP cells after 5 days by fluorometric analysis
    Transcriptional activity at human androgen receptor BF3 site stably transfected in eGFP-expressing human LNCAP cells after 5 days by fluorometric analysis
    		[PMID: 22047606]
    Oocyte IC50
    50.4 μM
    Compound: Triiodothyronine
    TP_TRANSPORTER: inhibition of Digoxin uptake in Xenopus laevis oocytes
    TP_TRANSPORTER: inhibition of Digoxin uptake in Xenopus laevis oocytes
    		[PMID: 11561088]
    Sf9 EC50
    0.5 nM
    Compound: T3
    Agonist activity at human THRalpha expressed in baculovirus infected SF9 cells after 15 mins by gel electrophoresis
    Agonist activity at human THRalpha expressed in baculovirus infected SF9 cells after 15 mins by gel electrophoresis
    		[PMID: 32356658]
    U2OS EC50
    11 nM
    Compound: T3
    Effect on human TRbeta transactivation activity in U2OS cells by luciferase reporter assay
    Effect on human TRbeta transactivation activity in U2OS cells by luciferase reporter assay
    		[PMID: 17988877]
    U2OS EC50
    2.4 nM
    Compound: T3
    Effect on human TRalpha transactivation activity in U2OS cells by luciferase reporter assay
    Effect on human TRalpha transactivation activity in U2OS cells by luciferase reporter assay
    		[PMID: 17988877]
    体外実験

    hepatocarcinomaLiothyronine (T3, 100 nM) stimulates the proliferation of hepatocarcinema cells in which TRβ1 is overexpressed[1]. Liothyronine binds to the human β1 thyroid hormone receptor (hTRβ1), and changes its conformation. Liothyronine promotes growth, induces differentiation and regualtes metabolic effects[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    リオチロニン 関連抗体
    臨床実験
    分子量

    650.97

    分子式

    C15H12I3NO4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC1=C(C=C(C=C1)OC2=C(C=C(C=C2I)C[C@@H](C(O)=O)N)I)I
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (76.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    1M NaOH : 50 mg/mL (76.81 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5362 mL 7.6808 mL 15.3617 mL
    5 mM 0.3072 mL 1.5362 mL 3.0723 mL
    10 mM 0.1536 mL 0.7681 mL 1.5362 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

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    濃度 （終了）

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    体積 （終了）

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.82%

    COA (245 KB) HNMR (252 KB) LCMS (257 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [1]

    Thyroid hormone depleted (Td) serum is prepared. The growth of hepatocarcinoma cells in methylcellulose is performed. To determine the effect of Liothyronine (T3) on the growth of cells, cells are plated at a density of 3 × l04 cells/60 mm dish on day 0, and incubated in medium containing 5% regular serum, 5% Td or 5% Td and 100 nM T3. The colony formation in methylcellulose is scored 3 weeks after initial plating[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / 1M NaOH 1 mM 1.5362 mL 7.6808 mL 15.3617 mL 38.4042 mL
    5 mM 0.3072 mL 1.5362 mL 3.0723 mL 7.6808 mL
    10 mM 0.1536 mL 0.7681 mL 1.5362 mL 3.8404 mL
    15 mM 0.1024 mL 0.5121 mL 1.0241 mL 2.5603 mL
    20 mM 0.0768 mL 0.3840 mL 0.7681 mL 1.9202 mL
    25 mM 0.0614 mL 0.3072 mL 0.6145 mL 1.5362 mL
    30 mM 0.0512 mL 0.2560 mL 0.5121 mL 1.2801 mL
    40 mM 0.0384 mL 0.1920 mL 0.3840 mL 0.9601 mL
    50 mM 0.0307 mL 0.1536 mL 0.3072 mL 0.7681 mL
    60 mM 0.0256 mL 0.1280 mL 0.2560 mL 0.6401 mL
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    Liothyronine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Liothyronine6893-02-3Triiodothyronine 3,3',5-Triiodo-L-thyronine T3T3T 3T-3Thyroid Hormone ReceptorEndogenous MetaboliteTHRthyroid hormoneTRαTRβInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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