2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Thyroid Hormone Receptor Endogenous Metabolite
  4. Liothyronine sodium

Liothyronine sodium  (Synonyms: Triiodothyronine sodium; 3,3',5-Triiodo-L-thyronine sodium; T3 sodium)

Cat. No.: HY-A0070 Purity: 99.63%
COA
SDS
Handling Instructions Technical Support

Liothyronine sodium is an active form of thyroid hormone. Liothyronine sodium is a potent thyroid hormone receptors TRα and TRβ agonist with Kis of 2.33 nM for hTRα and hTRβ, respectively.

For research use only. We do not sell to patients.

CAS No. : 55-06-1

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Customer Review

Based on 39 publication(s) in Google Scholar

Other Forms of Liothyronine sodium:

Liothyronine sodium Related Antibodies

Top Publications Citing Use of Products

39 Publications Citing Use of MCE Liothyronine sodium

WB
Cell Proliferation/Viability Assay
Cell Migration/Invasion Assay

    Liothyronine sodium purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    PGC1α mRNA levels in TRβ‐overexpressing ccRCC cell lines versus controls following Triiodothyronine (T3) treatment (100 nM for 48 h).

    Liothyronine sodium purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    CCK8 assay showing proliferation of TRβ-overexpressing cells treated with T3 (100 nM for 48 h).

    Liothyronine sodium purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    Transwell assay results comparing the migration of TRβ-overexpressing cells with control cells in the presence of T3 (100 nM for 48 h).

    Liothyronine sodium purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8.  [Abstract]

    C3H10T1/2 preadipocytes were treated with differentiation medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nM Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days and subsequently cultured in maintaining medium supplemented with 1 nM Triiodothyronine, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were cultured in complete medium until used for experiments. Representative immunoblots of PGC-1α and UCP1 in beige adipocytes treated as indicated.

    Liothyronine sodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 2;14(1):3208.  [Abstract]

    C3H10T1/2 preadipocytes were cultured in DMEM supplemented with 10% FBS, 1% P/S. For adipogenic differentiation, confluent C3H10T1/2 preadipocytes were incubated with complete medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nm Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days. Then, cells were changed to the complete medium supplemented with 1 nM T3, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were maintained in complete medium until used for experiments. Representative immunoblots of NRP1 and EPHA7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Liothyronine sodium is an active form of thyroid hormone. Liothyronine sodium is a potent thyroid hormone receptors TRα and TRβ agonist with Kis of 2.33 nM for hTRα and hTRβ, respectively[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Liothyronine (T3, 100 nM) sodium stimulates the proliferation of hepatocarcinema cells in which TRβ1 is overexpressed[1]. Liothyronine sodium binds to human β1 thyroid hormone receptor (hTRβ1), and changes its conformation. Liothyronine sodium promotes growth, induces differentiation and regualtes metabolic effects[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Liothyronine sodium Related Antibodies
    Molecular Weight

    672.96

    Formula

    C15H11I3NNaO4
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    N[C@@H](CC1=CC(I)=C(C(I)=C1)OC2=CC=C(O)C(I)=C2)C(O[Na])=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (74.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    0.1 M NaOH : 12.5 mg/mL (18.57 mM; ultrasonic and adjust pH to 11 with NaOH)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4860 mL 7.4299 mL 14.8597 mL
    5 mM 0.2972 mL 1.4860 mL 2.9719 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.71 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.71 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.63%

    SDS (393 KB)

    COA (245 KB) RP-HPLC (257 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Thyroid hormone depleted (Td) serum is prepared. The growth of hepatocarcinoma cells in methylcellulose is performed. To determine the effect of Liothyronine (T3) on the growth of cells, cells are plated at a density of 3 × 104 cells/60 mm dish on day 0, and incubated in medium containing 5% regular serum, 5% Td or 5% Td and 100 nM T3. The colony formation in methylcellulose is scored 3 weeks after initial plating[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    0.1 M NaOH / DMSO 1 mM 1.4860 mL 7.4299 mL 14.8597 mL 37.1493 mL
    5 mM 0.2972 mL 1.4860 mL 2.9719 mL 7.4299 mL
    10 mM 0.1486 mL 0.7430 mL 1.4860 mL 3.7149 mL
    15 mM 0.0991 mL 0.4953 mL 0.9906 mL 2.4766 mL
    DMSO 20 mM 0.0743 mL 0.3715 mL 0.7430 mL 1.8575 mL
    25 mM 0.0594 mL 0.2972 mL 0.5944 mL 1.4860 mL
    30 mM 0.0495 mL 0.2477 mL 0.4953 mL 1.2383 mL
    40 mM 0.0371 mL 0.1857 mL 0.3715 mL 0.9287 mL
    50 mM 0.0297 mL 0.1486 mL 0.2972 mL 0.7430 mL
    60 mM 0.0248 mL 0.1238 mL 0.2477 mL 0.6192 mL
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    Liothyronine sodium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Liothyronine55-06-1Triiodothyronine3,3',5-Triiodo-L-thyronineT3T 3T-3Thyroid Hormone ReceptorEndogenous MetaboliteTHRthyroid hormoneTRαTRβInhibitorinhibitorinhibit

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