Liothyronine (Synonyms: Triiodothyronine; 3,3',5-Triiodo-L-thyronine; T3)

Name: Liothyronine
Brand: MedChemExpress
SKU: HY-A0070A
Rating: 5.0 (39 reviews)

Cat. No.: HY-A0070A Purity: 99.82%: Data Sheet
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Liothyronine is an active form of thyroid hormone. Liothyronine binds to thyroid hormone receptors TRα and TRβ with K_is of 2.33 and 2.29 nM for hTRα and hTRβ, respectively. Liothyronine also binds to PVR and blocks the interaction of TIGIT/PVR.

For research use only. We do not sell to patients.

CAS No. : 6893-02-3

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100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 39 publication(s) in Google Scholar

Other Forms of Liothyronine:

Liothyronine-¹³C₆-1 In-stock
Liothyronine sodium In-stock
Liothyronine sodium hydrate In-stock
Liothyronine (GMP) In-stock
Liothyronine (Standard) In-stock
Liothyronine-¹³C₆ (hydrochloride) Get quote
Liothyronine-¹³C₉,¹⁵N Get quote
Liothyronine-d₃ Get quote
Liothyronine-d₄ Get quote
Liothyronine hydrochloride Get quote

39 Publications Citing Use of MCE Liothyronine

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

: Liothyronine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571. [Abstract]; PGC1α mRNA levels in TRβ‐overexpressing ccRCC cell lines versus controls following Triiodothyronine (T3) treatment (100 nM for 48 h).

: Liothyronine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571. [Abstract]; CCK8 assay showing proliferation of TRβ-overexpressing cells treated with T3 (100 nM for 48 h).

: Liothyronine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571. [Abstract]; Transwell assay results comparing the migration of TRβ-overexpressing cells with control cells in the presence of T3 (100 nM for 48 h).

: Liothyronine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8. [Abstract]; C3H10T1/2 preadipocytes were treated with differentiation medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nM Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days and subsequently cultured in maintaining medium supplemented with 1 nM Triiodothyronine, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were cultured in complete medium until used for experiments. Representative immunoblots of PGC-1α and UCP1 in beige adipocytes treated as indicated.

: Liothyronine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 2;14(1):3208. [Abstract]; C3H10T1/2 preadipocytes were cultured in DMEM supplemented with 10% FBS, 1% P/S. For adipogenic differentiation, confluent C3H10T1/2 preadipocytes were incubated with complete medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nm Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days. Then, cells were changed to the complete medium supplemented with 1 nM T3, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were maintained in complete medium until used for experiments. Representative immunoblots of NRP1 and EPHA7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC.

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Biological Activity
Protocol
Purity & Documentation
References
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Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	3.4 nM Compound: T3	Agonist activity at human THRalpha expressed in CHO cells after 24 hrs by luciferase assay Agonist activity at human THRalpha expressed in CHO cells after 24 hrs by luciferase assay	[PMID: 32356658]
CHO	EC₅₀	6.2 nM Compound: T3	Agonist activity at human THRbeta expressed in CHO cells after 24 hrs by luciferase assay Agonist activity at human THRbeta expressed in CHO cells after 24 hrs by luciferase assay	[PMID: 32356658]
CHO-K1	IC₅₀	0.24 nM Compound: 1	Binding affinity at human thyroid hormone receptor alpha 1 expressed in CHOK1 cells Binding affinity at human thyroid hormone receptor alpha 1 expressed in CHOK1 cells	[PMID: 17543524]
CHO-K1	IC₅₀	0.26 nM Compound: 1	Binding affinity at human thyroid hormone receptor beta-1 expressed in CHOK1 cells Binding affinity at human thyroid hormone receptor beta-1 expressed in CHOK1 cells	[PMID: 17543524]
CV-1	EC₅₀	0.002 μM Compound: T3	Agonist activity at human recombinant TRbeta1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay Agonist activity at human recombinant TRbeta1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay	[PMID: 24290064]
CV-1	EC₅₀	0.0021 μM Compound: T3	Agonist activity at human recombinant TRalpha1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay Agonist activity at human recombinant TRalpha1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay	[PMID: 24290064]
CV-1	EC₅₀	0.5 nM Compound: T3	Agonist activity at Xenopus laevis THRalpha transfected in green monkey CV1 cells after 48 hrs by reporter gene assay Agonist activity at Xenopus laevis THRalpha transfected in green monkey CV1 cells after 48 hrs by reporter gene assay	[PMID: 32356658]
CV-1	EC₅₀	1.2 nM Compound: T3	Agonist activity at human THRbeta expressed in green monkey CV1 cells after 24 hrs by luciferase assay Agonist activity at human THRbeta expressed in green monkey CV1 cells after 24 hrs by luciferase assay	[PMID: 32356658]
HEK-293T	EC₅₀	5.8 μM Compound: T3	Agonist activity at recombinant human pFA-CMV fused PPARgamma expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay Agonist activity at recombinant human pFA-CMV fused PPARgamma expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay	[PMID: 32356658]
HEK293	EC₅₀	0.41 nM Compound: T3	Agonist activity at human thyroid hormone receptor alpha expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human thyroid hormone receptor alpha expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HeLa	EC₅₀	2.4 nM Compound: T3	Effect on human TRalpha transactivation activity in HeLa cells by luciferase reporter assay Effect on human TRalpha transactivation activity in HeLa cells by luciferase reporter assay	[PMID: 17988877]
HeLa	EC₅₀	2.4 nM Compound: T3	Effect on human TRbeta transactivation activity in HeLa cells by luciferase reporter assay Effect on human TRbeta transactivation activity in HeLa cells by luciferase reporter assay	[PMID: 17988877]
LNCaP	IC₅₀	> 50 μM Compound: 6, T3	Transcriptional activity at human androgen receptor BF3 site stably transfected in eGFP-expressing human LNCAP cells after 5 days by fluorometric analysis Transcriptional activity at human androgen receptor BF3 site stably transfected in eGFP-expressing human LNCAP cells after 5 days by fluorometric analysis	[PMID: 22047606]
Oocyte	IC₅₀	50.4 μM Compound: Triiodothyronine	TP_TRANSPORTER: inhibition of Digoxin uptake in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of Digoxin uptake in Xenopus laevis oocytes	[PMID: 11561088]
Sf9	EC₅₀	0.5 nM Compound: T3	Agonist activity at human THRalpha expressed in baculovirus infected SF9 cells after 15 mins by gel electrophoresis Agonist activity at human THRalpha expressed in baculovirus infected SF9 cells after 15 mins by gel electrophoresis	[PMID: 32356658]
U2OS	EC₅₀	11 nM Compound: T3	Effect on human TRbeta transactivation activity in U2OS cells by luciferase reporter assay Effect on human TRbeta transactivation activity in U2OS cells by luciferase reporter assay	[PMID: 17988877]
U2OS	EC₅₀	2.4 nM Compound: T3	Effect on human TRalpha transactivation activity in U2OS cells by luciferase reporter assay Effect on human TRalpha transactivation activity in U2OS cells by luciferase reporter assay	[PMID: 17988877]

In Vitro

hepatocarcinomaLiothyronine (T3, 100 nM) stimulates the proliferation of hepatocarcinema cells in which TRβ1 is overexpressed^[1]. Liothyronine binds to the human β1 thyroid hormone receptor (hTRβ1), and changes its conformation. Liothyronine promotes growth, induces differentiation and regualtes metabolic effects^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

650.97

Formula

C₁₅H₁₂I₃NO₄

CAS No.

6893-02-3

Appearance

Solid

Color

White to off-white

SMILES

OC1=C(C=C(C=C1)OC2=C(C=C(C=C2I)C[C@@H](C(O)=O)N)I)I

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (76.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M NaOH : 50 mg/mL (76.81 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5362 mL	7.6808 mL	15.3617 mL
5 mM	0.3072 mL	1.5362 mL	3.0723 mL
10 mM	0.1536 mL	0.7681 mL	1.5362 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.82%

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Data Sheet (274 KB) SDS (394 KB)

COA (245 KB) HNMR (252 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Lin KH, et al. Stimulation of proliferation by 3,3',5-triiodo-L-thyronine in poorly differentiated human hepatocarcinoma cells overexpressing beta 1 thyroid hormone receptor. Cancer Lett. 1994 Oct 14;85(2):189-94. [Content Brief]

[2]. Bhat MK, et al. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3',5-triiodo-L-thyronine. Biochem Biophys Res Commun. 1993 Aug 31;195(1):385-92. [Content Brief]

[3]. Hiroaki Shiohara, et al. Discovery of novel indane derivatives as liver-selective thyroid hormone receptor β (TRβ) agonists for the treatment of dyslipidemia. Bioorg Med Chem. 2012 Jun 1;20(11):3622-34. [Content Brief]

[4]. Zhou X, et al. Repositioning liothyronine for cancer immunotherapy by blocking the interaction of immune checkpoint TIGIT/PVR. Cell Commun Signal. 2020 Sep 7;18(1):142. [Content Brief]

Cell Assay
^[1]

Thyroid hormone depleted (Td) serum is prepared. The growth of hepatocarcinoma cells in methylcellulose is performed. To determine the effect of Liothyronine (T3) on the growth of cells, cells are plated at a density of 3 × l0⁴ cells/60 mm dish on day 0, and incubated in medium containing 5% regular serum, 5% Td or 5% Td and 100 nM T3. The colony formation in methylcellulose is scored 3 weeks after initial plating^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Lin KH, et al. Stimulation of proliferation by 3,3',5-triiodo-L-thyronine in poorly differentiated human hepatocarcinoma cells overexpressing beta 1 thyroid hormone receptor. Cancer Lett. 1994 Oct 14;85(2):189-94. [Content Brief]

[2]. Bhat MK, et al. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3',5-triiodo-L-thyronine. Biochem Biophys Res Commun. 1993 Aug 31;195(1):385-92. [Content Brief]

[3]. Hiroaki Shiohara, et al. Discovery of novel indane derivatives as liver-selective thyroid hormone receptor β (TRβ) agonists for the treatment of dyslipidemia. Bioorg Med Chem. 2012 Jun 1;20(11):3622-34. [Content Brief]

[4]. Zhou X, et al. Repositioning liothyronine for cancer immunotherapy by blocking the interaction of immune checkpoint TIGIT/PVR. Cell Commun Signal. 2020 Sep 7;18(1):142. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / 1M NaOH	1 mM	1.5362 mL	7.6808 mL	15.3617 mL	38.4042 mL
	5 mM	0.3072 mL	1.5362 mL	3.0723 mL	7.6808 mL
	10 mM	0.1536 mL	0.7681 mL	1.5362 mL	3.8404 mL
	15 mM	0.1024 mL	0.5121 mL	1.0241 mL	2.5603 mL
	20 mM	0.0768 mL	0.3840 mL	0.7681 mL	1.9202 mL
	25 mM	0.0614 mL	0.3072 mL	0.6145 mL	1.5362 mL
	30 mM	0.0512 mL	0.2560 mL	0.5121 mL	1.2801 mL
	40 mM	0.0384 mL	0.1920 mL	0.3840 mL	0.9601 mL
	50 mM	0.0307 mL	0.1536 mL	0.3072 mL	0.7681 mL
	60 mM	0.0256 mL	0.1280 mL	0.2560 mL	0.6401 mL

Liothyronine Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Liothyronine6893-02-3Triiodothyronine 3,3',5-Triiodo-L-thyronine T3T3T 3T-3Thyroid Hormone ReceptorEndogenous MetaboliteTHRthyroid hormoneTRαTRβInhibitorinhibitorinhibit

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Liothyronine (Synonyms: Triiodothyronine; 3,3',5-Triiodo-L-thyronine; T3)

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Other Forms of Liothyronine:

Liothyronine Related Antibodies

39 Publications Citing Use of MCE Liothyronine

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View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

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Customer Review

Liothyronine Related Antibodies

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Complete Stock Solution Preparation Table

Liothyronine Related Classifications

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