2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Thyroid Hormone Receptor Endogenous Metabolite
  4. Liothyronine hydrochloride

Liothyronine hydrochloride  (Synonyms: Triiodothyronine hydrochloride; 3,3',5-Triiodo-L-thyronine hydrochloride; T3 hydrochloride)

Cat. No.: HY-A0070C
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Liothyronine hydrochloride is an active form of thyroid hormone. Liothyronine hydrochloride is a potent thyroid hormone receptors TRα and TRβ agonist with Kis of 2.33 nM for hTRα and hTRβ, respectively.

For research use only. We do not sell to patients.

CAS No. : 6138-47-2

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Liothyronine hydrochloride Related Antibodies

Top Publications Citing Use of Products

39 Publications Citing Use of MCE Liothyronine hydrochloride

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Cell Migration/Invasion Assay

    Liothyronine hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    PGC1α mRNA levels in TRβ‐overexpressing ccRCC cell lines versus controls following Triiodothyronine (T3) treatment (100 nM for 48 h).

    Liothyronine hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    CCK8 assay showing proliferation of TRβ-overexpressing cells treated with T3 (100 nM for 48 h).

    Liothyronine hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 9:e08571.  [Abstract]

    Transwell assay results comparing the migration of TRβ-overexpressing cells with control cells in the presence of T3 (100 nM for 48 h).

    Liothyronine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8.  [Abstract]

    C3H10T1/2 preadipocytes were treated with differentiation medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nM Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days and subsequently cultured in maintaining medium supplemented with 1 nM Triiodothyronine, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were cultured in complete medium until used for experiments. Representative immunoblots of PGC-1α and UCP1 in beige adipocytes treated as indicated.

    Liothyronine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 2;14(1):3208.  [Abstract]

    C3H10T1/2 preadipocytes were cultured in DMEM supplemented with 10% FBS, 1% P/S. For adipogenic differentiation, confluent C3H10T1/2 preadipocytes were incubated with complete medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM Dexamethasone, 850 nM Insulin, 1 nm Triiodothyronine, 125 nm Indomethacin and 1 μM Rosiglitazone for 2 days. Then, cells were changed to the complete medium supplemented with 1 nM T3, 850 nM Insulin, and 1 μM Rosiglitazone for another 2 days. Differentiated adipocytes at day 4 were maintained in complete medium until used for experiments. Representative immunoblots of NRP1 and EPHA7 in differentiated brown adipocytes transfected with si-Rbm3 or si-NC.

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    Description

    Liothyronine hydrochloride is an active form of thyroid hormone. Liothyronine hydrochloride is a potent thyroid hormone receptors TRα and TRβ agonist with Kis of 2.33 nM for hTRα and hTRβ, respectively[1][2][3].

    IC50 & Target

    TRβ1[1][2]
    In Vitro

    Liothyronine (T3, 100 nM) hydrochloride stimulates the proliferation of hepatocarcinema cells in which TRβ1 is overexpressed[1]. Liothyronine hydrochloride binds to human β1 thyroid hormone receptor (hTRβ1), and changes its conformation. Liothyronine hydrochloride promotes growth, induces differentiation and regualtes metabolic effects[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Liothyronine hydrochloride Related Antibodies
    Molecular Weight

    687.43

    Formula

    C15H13ClI3NO4
    CAS No.
    SMILES

    O=C([C@H](CC1=CC(I)=C(C(I)=C1)OC2=CC=C(C(I)=C2)O)N)O.Cl
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Liothyronine hydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Liothyronine6138-47-2Triiodothyronine3,3',5-Triiodo-L-thyronineT3T 3T-3Thyroid Hormone ReceptorEndogenous MetaboliteTHRthyroid hormoneTRαTRβInhibitorinhibitorinhibit

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