Characterizing the Neuron-Glial Interactions by the Co-cultures of Human iPSC-Derived Oligodendroglia and Neurons

  • Methods Mol Biol. 2023;2683:103-111. doi: 10.1007/978-1-0716-3287-1_9.
Gabriella Vulakh  1 ,  Xin Yang  2
Affiliations
  • 1. Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI, USA.
  • 2. Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI, USA. [email protected].
PMID: 37300770 DOI: 10.1007/978-1-0716-3287-1_9
Abstract

Induced pluripotent stem cell (iPSC) techniques have had considerable breakthroughs in modeling human neurological diseases. Multiple protocols inducing neurons, astrocytes, microglia, oligodendrocytes, and endothelial cells have been well-established thus far. However, these protocols have limitations, including the long time period to get cells of interest or the challenge of culturing more than one cell type simultaneously. Protocols for handling multiple cell types within a shorter time period are still being established. Here we describe a simple and reliable co-culture system to study interactions between neurons and oligodendrocyte precursor cells (OPC) in health and in disease.

Keywords
Co-culture; Induced pluripotent stem (iPS) cells; Neuron-glial interactions; Neurons; Oligodendrocyte precursor cells.
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