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Biotin

(Synonyms: Vitamin B7; Vitamin H; D-Biotin)
26 Cited Publications
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Based on 26 publication(s) in Google Scholar

Biotin, vitamin B7 and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

For research use only. We do not sell to patients.

  • Purity: 99.16%
  • CAS No.: 58-85-5
  • Formula: C10H16N2O3S
  • Molecular Weight:244.31
  • Storage:
    Powder   -20°C, 3 years , 4°C, 2 years ; In solvent   -80°C, 6 months , -20°C, 1 month
  • Biotin Chemical Structure
    Biotin Chemical Structure
  • Experimental Validation Results for Biotin
  • Experimental Validation Results for Biotin
  • Experimental Validation Results for Biotin
  • Experimental Validation Results for Biotin
  • Experimental Validation Results for Biotin
CAS No.: 58-85-5
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  • Biotin Chemical Structure
    Biotin Chemical Structure
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    Biotin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):1974-1990. [Abstract]

    Customer Validation
     Immunoblotting was performed to detect the presence of ADCY10 following co-incubation with cell lysate and subsequent precipitation of bound protein using streptavidin magnetic beads with either Bio-OLCarn or Biotin (20 mmol/L) for 12 h at 4 °C with continuous rotation.

  • 1/16

    Biotin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):1974-1990. [Abstract]

    Customer Validation
     Fluorescence intensity represents the colocalization of ADCY10 and Bio-OLCarn or Biotin (20 mmol/L) in HCC1806 cells overnight at 4 ℃; green: ADCY10, red: Bio-OLCarn.

  • 1/16

    Biotin purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2025 Mar 17;135(6):e185278. [Abstract]

    Customer Validation
     Biotin pull-down assay. SW620 cell protein lysates were incubated with biotin, biotin-RLY01 (B-RLY01; 10 μM), B-RLY01 (10 μM) + 100 μM RLY01 (10× molar excess), B-RLY01 (10 μM) + 200 μM RLY01 (20× molar excess) at room temperature for 3 hours by rotation.

  • 1/16

    Biotin purchased from MedChemExpress. Usage Cited in: Cell Discov. 2025 Sep 30;11(1):80. [Abstract]

    Customer Validation
     Biotin (50 μM, 3 h). MDA-MB-231 cells were treated with indicated drugs for 3 h, and biotin was pulled down. Interacting proteins were stained with Coomassie brilliant blue and K48 polyUB was detected by western blotting

  • 1/16

    Biotin purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 May 22;10(15):6839-6853. [Abstract]

    Customer Validation
     Immunoblot with anti-NCOR2, anti-ACACA, anti-FASN, anti-CAD, and anti-α-Tubulin antibodies of protein precipitated by streptavidin beads from MDA-MB-231 cell lysates in the presence of B-Emodin or Biotin (10 µM) for 5 h at 4 ℃ with rotation.

  • Biotin