1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Biotin

Biotin  (Synonyms: Vitamin B7; Vitamin H; D-Biotin)

Cat. No.: HY-B0511 Purity: 99.62%
COA Handling Instructions

Biotin (Vitamin B7) is a water-soluble B vitamin and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

For research use only. We do not sell to patients.

Biotin Chemical Structure

Biotin Chemical Structure

CAS No. : 58-85-5

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10 mM * 1 mL in DMSO USD 61 In-stock
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1 g USD 92 In-stock
5 g USD 330 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Biotin:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

Biotin (Vitamin B7) is a water-soluble B vitamin and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids[1][2][3].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively[4].
Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmax of 27.34 pmol/mg protein/min[4].
Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 µM)-induced cell death and reduces cell adhesion[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[5]

Cell Line: Murine oligodendrocyte 158N cells
Concentration: 1, 10, 100, 1000 nM
Incubation Time: 24 hours
Result: Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status.
Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration.
In Vivo

Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice[6].
Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g)[6]
Dosage: 15 mg/kg/d
Administration: Oral gavage; 12 days
Result: Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage.
Animal Model: Grass carp (117±0.5 g)[7]
Dosage: 0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg
Administration: Oral gavage; 70 days
Result: Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM).
Reduced the mRNA levels of anti-microbial substances.
Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling.
Clinical Trial
Molecular Weight

244.31

Appearance

Solid

Formula

C10H16N2O3S

CAS No.
SMILES

O=C(O)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (409.32 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0932 mL 20.4658 mL 40.9316 mL
5 mM 0.8186 mL 4.0932 mL 8.1863 mL
10 mM 0.4093 mL 2.0466 mL 4.0932 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

  • 4.

    Add each solvent one by one:  PBS

    Solubility: 1.96 mg/mL (8.02 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.80%

References
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Biotin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Biotin
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HY-B0511
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