1. Immunology/Inflammation
    Metabolic Enzyme/Protease
    Neuronal Signaling
    Membrane Transporter/Ion Channel
  2. Glucocorticoid Receptor
    Endogenous Metabolite
    iGluR
  3. Corticosterone

Corticosterone  (Synonyms: 17-Deoxycortisol; 11β,21-Dihydroxyprogesterone; Kendall's compound B)

Cat. No.: HY-B1618 Purity: 99.63%
COA Handling Instructions

Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect.

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Corticosterone Chemical Structure

Corticosterone Chemical Structure

CAS No. : 50-22-6

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Customer Review

Based on 14 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Corticosterone purchased from MCE. Usage Cited in: Sci Adv. 2022 Nov 11;8(45):eadd7063.  [Abstract]

    Corticosterone (CORT) (10 µM, 24 h) is used to produce a cell stress model on the primary culture hippocampal neurons from rats. The decreased NHE1 expression levels in primary cell culture induced by CORT treatment are not affected by chloroquine (Fig. D) but significantly reversed with MG132 pretreatment (Fig. E).
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    Description

    Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect[1][2][3][4].

    IC50 & Target

    AMPA Receptor

     

    Human Endogenous Metabolite

     

    In Vitro

    Corticosterone (100 nM; 30 min) via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane[1].
    Corticosterone (CORT) (1 µM; 48 h) shows good immunosuppressive properties (functionally compromises maturation of BMDC), which impairs LPS-induced up-regulation of maturation-associated markers (MHC class II, B7.2, B7.1 and CD40)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: HEK293 cells
    Concentration: 100 nM
    Incubation Time: 30 min
    Result: Caused a significant enhancement of mEPSC amplitude (mEPSC represents the postsynaptic response to release of individual vesicles of glutamate).
    Increased the transmission of glutamatergic, and increased synaptic AMPAR currents via a Rab4-dependent mechanism.
    Profoundly increased surface GluR1 cluster density, cluster size and cluster fluorescence intensity.
    Significantly increased the amount of Rab4 that binded to WT-GDI, S45A-GDI, or S121A-GDI but not S213A-GDI.
    Induced the phosphorylation of GST-WTGDI, GST-S45AGDI, and GST-S121AGDI, but not GST-S213AGDI, and this effect was blocked in cells transfected with SGK1 small interfering RNA.
    Increased AMPAR surface expression via a mechanism dependent on GDI phosphorylation.

    Cell Viability Assay[2]

    Cell Line: BMDC cells
    Concentration: 1 µM
    Incubation Time: 48 h
    Result: Completely blocked the expression of MHC class II and B7.2 that induced by LPS, and maximally impaired BMDC cells maturation at 12 h.
    Reduced B7.1 by 50%, and slightly down-regulated CD40.
    In Vivo

    Corticosterone results in a marked reduction in the ability of BMDC cells to prime naive CD8+ T cells in vivo[2].
    Corticosterone (0.03 or 1 mg/kg; s.c.; single) downregulates expression of BDNF mRNA in dentate gyrus and CA1 of rats[3].
    Corticosterone (2.6 mg/kg; in animal feedings; 8 days) restores ethanol intake and preference to approximately normal preoperative levels in adrenalectomy (ADX) rats[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Wistar rats (150-170 g; adrenalectomized)[3].
    Dosage: 0.03 or 1 mg/kg
    Administration: Subcutaneous injection; single.
    Result: Decreased expression of BDNF mRNA in dentate gyrus, with 25% and 50% lower for dosages of 0.03 and 1 mg/kg, respectively (3 h after administration).
    Reduced approximately 40% BDNF mRNA level as compared to the t=0 h control group (3 h after administration), but the level increased by 100% when 12 h after administration (compared to t=3 h and t=6 h group).
    Animal Model: Male Wistar rats (3-week-old; adrenalectomized)[4].
    Dosage: 2.6 mg/kg
    Administration: In animal feedings; 8 days.
    Result: Restored ethanol intake and preference of adrenalectomy (ADX) rats to approximately normal preoperative levels and to the levels observed in the sham-operated group (SH) rats.
    Clinical Trial
    Molecular Weight

    346.46

    Formula

    C21H30O4

    CAS No.
    SMILES
    Structure Classification
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (288.63 mM; Need ultrasonic)

    Ethanol : 14.29 mg/mL (41.25 mM; Need ultrasonic)

    H2O : 0.67 mg/mL (1.93 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8863 mL 14.4317 mL 28.8634 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL
    10 mM 0.2886 mL 1.4432 mL 2.8863 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  20% HP-β-CD    5% Cremophor EL

      Solubility: 5 mg/mL (14.43 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  20% HP-β-CD in saline

      Solubility: 4 mg/mL (11.55 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

    • 5.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

    • 6.

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 2 mg/mL (5.77 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

    • 7.

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 1.43 mg/mL (4.13 mM); Clear solution

    • 8.

      Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 1.43 mg/mL (4.13 mM); Clear solution

    • 9.

      Add each solvent one by one:  10% EtOH    90% corn oil

      Solubility: ≥ 1.43 mg/mL (4.13 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.70%

    References
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Corticosterone
    Cat. No.:
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