5-Aminolevulinic acid (Synonyms: 5-ALA; δ-Aminolevulinic acid; 5-Amino-4-oxopentanoic acid)

Cat. No.: HY-W000450: FAQs
Data Sheet Handling Instructions

Molarity Calculator

5-Aminolevulinic acid (5-ALA) is a non-protein amino acid that plays a rate-limiting role in heme biosynthesis.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (5-Aminolevulinic acid hydrochloride) that retains the same biological activity.

For research use only. We do not sell to patients.

5-Aminolevulinic acid Chemical Structure

CAS No. : 106-60-5

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Other In-stock Forms of 5-Aminolevulinic acid:

Other Forms of 5-Aminolevulinic acid:

5-Aminolevulinic acid hydrochloride In-stock
5-Aminolevulinic acid-¹³C Get quote

10 Publications Citing Use of MCE 5-Aminolevulinic acid

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Aminolevulinic acid (5-ALA) is a non-protein amino acid that plays a rate-limiting role in heme biosynthesis.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

5-Aminolevulinic acid (5-ALA) enhances the immune response of L. vannamei to V. parahaemolyticus, upregulates immune- and defense-related genes, and enhances aerobic energy metabolism, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

131.13

Formula

C₅H₉NO₃

CAS No.

106-60-5

SMILES

O=C(O)CCC(CN)=O

Structure Classification

Ketones, Aldehydes, Acids

Amino acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (762.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.6260 mL	38.1301 mL	76.2602 mL
5 mM	1.5252 mL	7.6260 mL	15.2520 mL
10 mM	0.7626 mL	3.8130 mL	7.6260 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (19.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (19.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (19.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Pedrosa-Gerasmio IR, et al. Effects of 5-Aminolevulinic Acid on Gene Expression, Immunity, and ATP Levels in Pacific White Shrimp, Litopenaeus vannamei. Mar Biotechnol (NY). 2018 Aug 25. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.6260 mL	38.1301 mL	76.2602 mL	190.6505 mL
	5 mM	1.5252 mL	7.6260 mL	15.2520 mL	38.1301 mL
	10 mM	0.7626 mL	3.8130 mL	7.6260 mL	19.0650 mL
	15 mM	0.5084 mL	2.5420 mL	5.0840 mL	12.7100 mL
	20 mM	0.3813 mL	1.9065 mL	3.8130 mL	9.5325 mL
	25 mM	0.3050 mL	1.5252 mL	3.0504 mL	7.6260 mL
	30 mM	0.2542 mL	1.2710 mL	2.5420 mL	6.3550 mL
	40 mM	0.1907 mL	0.9533 mL	1.9065 mL	4.7663 mL
	50 mM	0.1525 mL	0.7626 mL	1.5252 mL	3.8130 mL
	60 mM	0.1271 mL	0.6355 mL	1.2710 mL	3.1775 mL
	80 mM	0.0953 mL	0.4766 mL	0.9533 mL	2.3831 mL
	100 mM	0.0763 mL	0.3813 mL	0.7626 mL	1.9065 mL

5-Aminolevulinic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Aminolevulinic acid106-60-55-ALA δ-Aminolevulinic acid 5-Amino-4-oxopentanoic acidEndogenous MetaboliteInhibitorinhibitorinhibit

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