1. GPCR/G Protein
  2. Adenosine Receptor

Adenosine 5'-monophosphate monohydrate (Synonyms: 5'-AMP monohydrate)

Cat. No.: HY-A0181A Purity: 99.07%
Handling Instructions

Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist.

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Adenosine 5'-monophosphate monohydrate Chemical Structure

Adenosine 5'-monophosphate monohydrate Chemical Structure

CAS No. : 18422-05-4

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10 mM * 1 mL in Water USD 55 In-stock
Estimated Time of Arrival: December 31
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Other Forms of Adenosine 5'-monophosphate monohydrate:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist.

IC50 & Target

Adenosine A1 receptor[1]

In Vitro

Adenosine 5'-monophosphate monohydrate is an adenosine A1 receptor agonist[1]. Adenosine 5'-monophosphate monohydrate (5′-AMP)-induced cell toxicity is negligible at concentrations of 25 to 400 μM in RAW264.7 cells. Adenosine 5'-monophosphate monohydrate significantly attenuates the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in RAW264.7 cells. The dose-dependence of TNF-α and IL-6 mRNA show that at concentration of 400 μM, Adenosine 5'-monophosphate monohydrate exhibits the maximum inhibition. Exposure of cells to Adenosine 5'-monophosphate monohydrate significantly reduces recruitment of NF-κB p65 to TNF-α, IL-6, and IL-1β gene promoters[2].

In Vivo

C57BL/6J mice treated with Adenosine 5'-monophosphate monohydrate (5′-AMP) markedly increases hepatic adenosine level. Survival rate in PBS-treated mice (n=15) is 60% (8 h) and 33.3% (24 h), whereas survival rate in Adenosine 5'-monophosphate monohydrate-treated mice (n=15) is 100% (8 h) and 93.3% (24 h). Serum aspartate transaminase (AST) and alanine transaminase (ALT) levels are significantly lowered in the Adenosine 5'-monophosphate monohydrate group than in the vehicle group. The area and extent of necrosis is attenuated and the infiltration of inflammatory cells is reduced in the Adenosine 5'-monophosphate monohydrate group[2].

Clinical Trial
Solvent & Solubility
In Vitro: 

H2O : 6.4 mg/mL (17.52 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7379 mL 13.6896 mL 27.3793 mL
5 mM 0.5476 mL 2.7379 mL 5.4759 mL
10 mM 0.2738 mL 1.3690 mL 2.7379 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[2]

RAW264.7 cells are plated in 100-mm dishes and stimulated with lipopolysaccharide (LPS) for 1 h. Cross-linked chromatin are sonicated into DNA fragment of 0.5 to 1.0 kb. For in vitro biochemical experiments, the sonicated cell lysates are incubated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) at 37°C for 30 min. Then chromatin is precleared with 50% salmon sperm DNA-saturated protein A agarose beads and immunoprecipitated with 5 μg of antibody[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[2]

The RAW264.7 cells (a murine macrophage cell line) are maintained in supplemented RPMI 1640 in an atmosphere at 90% humidity containing 5% CO2 at 37°C. At the end of the pre-incubated period, cells are rinsed with PBS and the medium is supplemented with RPMI 1640 without fetal bovine serum. The cells are pretreated with Adenosine 5'-monophosphate monohydrate (5′-AMP) (400 μM) for 30 min[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Male WT C57BL/6J mice are used at 8 to 10 weeks of age with a body weight of 20 to 25 g in this work. For Adenosine 5'-monophosphate monohydrate (5′-AMP)-pretreated survival experiment, WT mice are randomly divided into two groups (n=15 in each group). Adenosine 5'-monophosphate monohydrate (5 mg/20 g body weight i.p.) or PBS is administered to mice. For general experiment, WT mice are randomly divided into different groups (n=5 in each group): (1) vehicle/vehicle group; (2) Adenosine 5'-monophosphate monohydrate/vehicle group. Mice are killed and the blood is collected from the carotid artery. The liver of each mouse is removed immediately and then is kept at -80°C until analyzed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

365.24

Formula

C₁₀H₁₆N₅O₈P

CAS No.

18422-05-4

SMILES

O=P(O)(OC[[email protected]@H]1[[email protected]]([[email protected]]([[email protected]](N2C=NC3=C2N=CN=C3N)O1)O)O)O.O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Adenosine 5'-monophosphate monohydrate
Cat. No.:
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Adenosine 5'-monophosphate monohydrate

Cat. No.: HY-A0181A